Basic conditions for the drug selection and transient gene expression in the cultured cell line ofBombyx mori

1992 ◽  
Vol 28 (11-12) ◽  
pp. 779-781 ◽  
Author(s):  
Kazuhiro Okano ◽  
Naoko Miyajima ◽  
Naoko Takada ◽  
Masahiko Kobayashi ◽  
Hideaki Maekawa
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Transient Gene Expression ◽  
Drug Selection ◽  
Cultured Cell ◽  
Cultured Cell Line

scholarly journals cDNA identification and gene expression of two types of acetylcholinesterases in a cultured cell line of Aedes albopictus, compared to mosquito whole body extracts

2006 ◽  
Vol 41 (3) ◽  
pp. 445-453 ◽  
Author(s):  
Hiroshi Mizuno ◽  
Takashi Tomita ◽  
Shinji Kasai ◽  
Osamu Komagata ◽  
Shigeo Imanishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Aedes Albopictus ◽  
Whole Body ◽  
Cultured Cell ◽  
Cultured Cell Line

Further studies on the enzymic pattern of a cultured cell line from liver

1959 ◽  
Vol 31 (1) ◽  
pp. 268-269 ◽  
Author(s):  
Victor H. Auerbach ◽  
Duard L. Walker
Keyword(s):  
Cell Line ◽  
Cultured Cell ◽  
Cultured Cell Line

Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency

1982 ◽  
Vol 2 (9) ◽  
pp. 1145-1154
Author(s):  
Y M Shen ◽  
R R Hirschhorn ◽  
W E Mercer ◽  
E Surmacz ◽  
Y Tsutsui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Thymidine Kinase ◽  
Mammalian Cells ◽  
High Efficiency ◽  
Transient Gene Expression ◽  
Simian Virus 40 ◽  
Temperature Sensitive ◽  
Enzymatic Function ◽  
Very High

We have developed a procedure that gives a very high efficiency of transfection in mammalian cells with low-molecular-weight DNA (approximately 10(4) base pairs). The procedure uses cells in suspension that are shocked with polyethylene glycol 4 h after replating. We compared this transfection technique to the standard technique involving manual microinjection of DNA into the nuclei of mammalian cells, using recombinant plasmids containing the simian virus 40 A gene or the herpes simplex virus thymidine kinase gene or both. The efficiency of transfection depends on a number of variables, the most important of which is the difference in transfectability of different cell lines. In our laboratory, the cell line that had the highest efficiency of transfection was tk-ts13, which is derived from baby hamster kidney cells that are deficient in thymidine kinase and temperature sensitive for growth. Under the appropriate conditions, as many as 70% of these cells can be transfected so that transient gene expression can be detected. With the manual microinjection technique, gene expression is independent of the cell line used and occurs faster than after transfection. The results suggest that the critical stage in transfection is the delivery of DNA molecules to the nucleus. Our experiments also indicate that an enzymatic function, in our case, thymidine kinase activity, gives a higher percentage of positive transfectants than when proteins are visualized only by indirect immunofluorescence. The transfection procedure described in this paper is simple and reproducible and, although less efficient than microinjection, ought to be useful in phenotypic and genotypic studies in which transfer of genes to a large number of cells is desirable.

scholarly journals Trifluoperazine inhibits phagocytosis in a macrophagelike cultured cell line.

1981 ◽  
Vol 91 (3) ◽  
pp. 798-802 ◽  
Author(s):  
S B Horwitz ◽  
G H Chia ◽  
C Harracksingh ◽  
S Orlow ◽  
S Pifko-Hirst ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Cyclic Nucleotide ◽  
Cellular Function ◽  
Inhibitory Effects ◽  
Cultured Cell ◽  
Sulfonamide Derivative ◽  
Cultured Cell Line ◽  
Macrophagelike Cell

Trifluoperazine, a drug that binds to Ca2+-calmodulin and inhibits its interaction with other proteins, was found to inhibit growth and phagocytosis in a macrophagelike cell line, J774.16. Both effects were reversible and occurred at the same concentrations of drug (25--50 microM) that inhibited the activation of cyclic nucleotide phosphodiesterase by calmodulin in vitro. Fc-mediated phagocytosis was also depressed by W-7, a sulfonamide derivative that inhibits the activity of Ca2+-calmodulin. In contrast, taxol, a drug that stabilizes cellular microtubules, had no effect on Fc-mediated phagocytosis although it inhibited cell growth at nanomolar concentrations. The inhibitory effects of trifluoperazine and W-7 on phagocytosis suggest that calmodulin may be involved in this complex cellular function.

Alpha-fetoprotein-producing gastric carcinoma: Biological properties of a cultured cell line

1995 ◽  
Vol 30 (5) ◽  
pp. 589-598 ◽  
Author(s):  
Morimasa Sekiguchi ◽  
Yuzo Fujii ◽  
Atsuko Saito ◽  
Toshimitsu Suzuki ◽  
Yasuko Shiroko ◽  
...  
Keyword(s):  
Gastric Carcinoma ◽  
Cell Line ◽  
Biological Properties ◽  
Alpha Fetoprotein ◽  
Cultured Cell ◽  
Cultured Cell Line

Transient gene expression in the cultured cell and silk gland of silkworm,

1989 ◽  
Vol 27 ◽  
pp. 89
Author(s):  
Hideaki Maekawa ◽  
Hideo Yamauchi ◽  
Naoko Miyajima ◽  
Keiko Ohta ◽  
Kazuhiro Okano ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Gene Expression ◽  
Silk Gland ◽  
Cultured Cell
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