Fish embryo culture: Migration and spreading of zebrafish (Brachydanio rerio) pigmented retinal epithelium

In Vitro ◽  
1981 ◽  
Vol 17 (8) ◽  
pp. 701-705 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Embryo Culture ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Retinal Epithelium

Fish embryo culture: cardiac monolayers and contractile activity in embryo explants from the zebrafish, Brachydanio rerio

1984 ◽  
Vol 62 (5) ◽  
pp. 878-885 ◽  
Author(s):  
Hans Willer Laale
Keyword(s):  
Embryo Culture ◽  
Nutrient Medium ◽  
Contractile Activity ◽  
Functional Differentiation ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Embryo Cultures ◽  
Culture Substrate ◽  
First Time ◽  
Cardiac Mesoderm

Stage 16+ axial isolates from embryos of the zebrafish, Brachydanio rerio (Hamilton-Buchanan), were separated from their yolk spheres and epidermis. Rhombencephalic level explants, with intact bilateral cardiac rudiments, were maintained in nutrient medium for 20 days at 24 ± 2 °C. All embryo cultures became attached to the culture substrate and underwent subsequent differentiation. Ten out of 25 explants showed bilateral migrations of determined but initially undifferentiated cardiac mesoderm. The progressive structural and functional differentiation of zebrafish cardiac monolayers and intact rudiments are described for the first time.

Fish embryo culture: rhombencephalic neuritic outgrowth in explanted axial cords from the zebrafish Brachydanio rerio (Hamilton-Buchanan)

1982 ◽  
Vol 60 (12) ◽  
pp. 3215-3219 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Embryo Culture ◽  
Fiber Bundles ◽  
Target Cells ◽  
Neural Cells ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Mammalian Tissues ◽  
Neuritic Outgrowth ◽  
Close Contacts

Fifteen stage 17 axial cords from the zebrafish Brachydanio rerio were stripped of epidermis and separated from their yolk spheres. Posterior halves, from the mid-rhombencephalic level to the caudal knob, were maintained in nutrient medium for 30 days at 24 ± 2 °C. The study describes preliminary observations on rhombencephalic neuritic outgrowth and associations with non-neural cells from 7 to 30 days of culture. Nerve fiber bundles increased in number and complexity with time and displayed heterogeneous branching behaviour. Frequent multiple close contacts with individual non-neural cells were observed, but selectivity of attraction to specific target cells was not verified. The findings are compared with similar contacts reported for mammalian tissues in vitro.

Fish embryo culture: observations on axial cord differentiation in presomitic isolates of the zebrafish Brachydanio rerio (Hamilton-Buchanan)

1982 ◽  
Vol 60 (7) ◽  
pp. 1710-1721 ◽  
Author(s):  
Hans W. Laale
Keyword(s):  
Embryo Culture ◽  
Nutrient Medium ◽  
Brachydanio Rerio ◽  
Fish Embryo ◽  
Fish Embryos ◽  
Meristic Variation

Eighteen pre- and post-rhombencephalic axial isolates from zebrafish, Brachydanio rerio, presomitic embryos divested of epidermis and yolk were maintained in a nutrient medium for 14 days at 24 ± 2 °C. The paper focuses on the differentiation of the notochord and metamerism of axial somites in culture. The technique is proposed as a possible means for studying parameters pertaining to meristic variation in developing fish embryos.

scholarly journals Toxic or Not Toxic, That Is the Carbon Quantum Dot’s Question: A Comprehensive Evaluation with Zebrafish Embryo, Eleutheroembryo, and Adult Models

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1598
Author(s):  
Chih-Yu Chung ◽  
Yu-Ju Chen ◽  
Chia-Hui Kang ◽  
Hung-Yun Lin ◽  
Chih-Ching Huang ◽  
...  
Keyword(s):  
Zebrafish Embryo ◽  
Comprehensive Evaluation ◽  
Aquatic Organisms ◽  
Carbon Quantum Dots ◽  
Adult Zebrafish ◽  
Fish Embryo ◽  
Wide Range ◽  
Different Types ◽  
Low Toxicity

Carbon quantum dots (CQDs) are emerging novel nanomaterials with a wide range of applications and high biocompatibility. However, there is a lack of in-depth research on whether CQDs can cause acute or long-term adverse reactions in aquatic organisms. In this study, two different types of CQDs prepared by ammonia citrate and spermidine, namely CQDAC and CQDSpd, were used to evaluate their biocompatibilities. In the fish embryo acute toxicity test (FET), the LD50 of CQDAC and CQDSpd was about 500 and 100 ppm. During the stage of eleutheroembryo, the LD50 decreased to 340 and 55 ppm, respectively. However, both CQDs were quickly eliminated from embryo and eleutheroembryo, indicating a lack of bioaccumulation. Long-term accumulation of CQDs was also performed in this study, and adult zebrafish showed no adverse effects in 12 weeks. In addition, there was no difference in the hatchability and deformity rates of offspring produced by adult zebrafish, regardless of whether they were fed CQDs or not. The results showed that both CQDAC and CQDSpd have low toxicity and bioaccumulation to zebrafish. Moreover, the toxicity assay developed in this study provides a comprehensive platform to assess the impacts of CQDs on aquatic organisms in the future.

Sign in / Sign up

Export Citation Format

Share Document