Modulation of actin mRNAs in cultured vascular cells by matrix components and TGF-β1

1989 ◽  
Vol 25 (5) ◽  
pp. 424-434 ◽  
Author(s):  
Olivier Kocher ◽  
Joseph A. Madri
Keyword(s):  
Vascular Cells ◽  
Matrix Components ◽  
Tgf Β1

scholarly journals Inhibitory Effect of the LY2109761 on the Development of Human Keloid Fibroblasts

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiuxia Wang ◽  
Chuan Gu ◽  
Feng Shang ◽  
Rui Jin ◽  
Jia Zhou ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Transforming Growth Factor ◽  
Scar Tissue ◽  
Inhibitory Effect ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Hypertrophic Scar Tissue ◽  
Β Receptor ◽  
Tgf Β1 ◽  
Keloid Fibroblasts

Keloids are scars characterized by abnormal proliferation of fibroblasts and overproduction of extracellular matrix components including collagen. We previously showed that LY2109761, a transforming growth factor- (TGF-) β receptor inhibitor, suppressed the secretion of matrix components and slowed the proliferation of fibroblasts derived from human hypertrophic scar tissue. However, the exact mechanism underlying this effect remains unclear. Here, we replicated the above results in keloid-derived fibroblasts and show that LY2109761 promoted apoptosis, decreased the phosphorylation of Smad2 and Smad3, and suppressed TGF-β1. These results suggest that the development and pathogenesis of keloids are positively regulated by the Smad2/3 signaling pathway and the upregulation of TGF-β1 receptors. LY2109761 and other inhibitors of these processes may therefore serve as therapeutic targets to limit excessive scarring after injury.

scholarly journals TGF-β1 autocrine signalling and enamel matrix components

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Saeko Kobayashi-Kinoshita ◽  
Yasuo Yamakoshi ◽  
Kazuo Onuma ◽  
Ryuji Yamamoto ◽  
Yoshinobu Asada
Keyword(s):  
Enamel Matrix ◽  
Matrix Components ◽  
Autocrine Signalling ◽  
Tgf Β1

Laminins and TGF-β maintain cell polarity and functionality of human gastric glandular epithelium

2002 ◽  
Vol 282 (4) ◽  
pp. C873-C884 ◽  
Author(s):  
Jean-René Basque ◽  
Pierre Chailler ◽  
Daniel Ménard
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Cell Polarity ◽  
Glandular Epithelium ◽  
Northern Hybridization ◽  
Type I ◽  
Integrin Subunit ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Tgf Β1

The human gastric glandular epithelium produces a gastric lipase enzyme (HGL) that plays an important role in digestion of dietary triglycerides. To assess the involvement of extracellular matrix components and transforming growth factor-β1 (TGF-β1) in the regulation of this enzymic function, normal gastric epithelial cells were cultured on collagen type I, Matrigel, and laminins (LN)-1 and -2 with or without TGF-β1. Epithelial morphology and HGL expression were evaluated using microscopy techniques, enzymic assays, Western blot, Northern hybridization, and RT-PCR. A correlation was observed between the cell polarity status and the level of HGL expression. TGF-β1 alone or individual matrix components stimulated cell spreading and caused a downfall of HGL activity and mRNA. By contrast, Matrigel preserved the morphological features of differentiated epithelial cells and maintained HGL expression. The combination of LNs with TGF-β1 (two constituents of Matrigel) exerted similar beneficial effects on epithelial cell polarity and evoked a 10-fold increase of HGL levels that was blunted by a neutralizing antibody against the α2-integrin subunit and by mitogen-activated protein kinase (MAPK) inhibitors PD-98059 (p42/p44) or SB-203580 (p38). This investigation demonstrates for the first time that a powerful synergism between a growth factor and basement membrane LNs positively influences cell polarity and functionality of the human gastric glandular epithelium through an activation of the α2β1-integrin and effectors of two MAPK pathways.

Structure, compositon and function of the dermal-epidermal junction: Relevance in cutaneous disease and diagnosis

1989 ◽  
Vol 47 ◽  
pp. 868-869
Author(s):  
K. A. Holbrook
Keyword(s):  
Basement Membrane ◽  
Epidermolysis Bullosa ◽  
Normal Skin ◽  
Lamina Densa ◽  
Inherited Disorders ◽  
Ultrastructural Studies ◽  
Type Iv ◽  
Type Vii Collagen ◽  
Attachment Structures ◽  
Matrix Components

The dermal-epidermal junction (DEJ), or basement membrane rone, is the boundary between the epithelial and mesenchymal compartments of the skin; epidermal and fibroblastic cells in these two regions collaborate to synthesire its components. Ultrastructural studies (TEM and SEM) have defined a series of planes or layers (basal epidermal, lamina lucida, lamina densa, sublamina densa) and the morphology and density of attachment structures (hemidesmosomes, anchoring filaments, anchoring fibrils and anchoring plaques) in this region of normal skin. Change in structure of the DEJ provides information about the history of the tissue; reduplication of the lamina densa, for example, indicates a site of cell detachment or migration, or remodelling that accompanies repair of focal damage. In normal skin the structure of the DEJ is stable; in pathologic conditions it can be compromised by the congenital absence of certain structures or antigens (e.g., in the inherited disorders, epidermolysis bullosa [EB]) or by enzymatic degradation (e.g., in tumor invasion). Dissolution of the DEJ can also occur normally during the formation of epidermal appendages (e.g., hair follicles) and as melanocytes and Langerhans cells migrate into the epidermis during development.Biochemical and immunohisto/cytochemical studies have identified more than 20 molecules at the DEJ. These include well known matrix molecules (e.g., types IV and V collagen, laminin and fibronectin) and skin-specific antigens. The latter have been identified by autoantibodies or specific polyclonal or monoclonal antibodies raised against the skin, cultured cells and other epithelia. Some of the molecules of the DEJ are are present in basement membrane zones of many epithelia and thus are considered ubiquitous components (type IV, V, laminin, fibronectin, nidogen, entactin, HSPG, LDA-1, CSP [3B3]). All of them (that have been investigated in developing skin) appear ontogenetically as early as human embryonic tissue can be obtained and their expression is typically normal in patients with EB. The known properties of many of these molecules (particularly the matrix components) suggest functions they might impart to the DEJ: support of an epithelium (type IV collagen), regulation of permeability (heparan sulfate proteoglycan) or facilitation of cell attachment (fibronectin) and movement (laminin). Another group of matrix components and antigens of the DEJ includes molecules that are skin-specific or characteristic of stratified squamous epithelia (type VII collagen=LH 7:2 antigen, bullous pemphigoid antigen, AA3, GB3, KF-1,19-DEJ-1, epidermolysis bullosa acquisita antigen [EBA], AF-1 and AF-2, cicatricial pemphigoid antigen [CPA]) . These molecules are expressed in the DEJ later in development than the first group of molecules, in conjunction with the morphologic appearance of the structure they represent. Their appearance is also coordinated with specific developmental events (e.g., epidermal stratification) and the expression of molecules of differentiation in the epidermis and dermis. One or more of them is typically absent or reduced in expression in the skin of patients with heritable disorders affecting this region. There is no apparent correlation between the location of molecules in the DEJ and the stability of their expression.

Thrombin induced TGF-β1 pathway: a cause of communicating hydrocephalus post subarachnoid hemorrhage

2010 ◽  
Vol 34 (8) ◽  
pp. S19-S19
Author(s):  
Tong Li ◽  
Peng Zhang ◽  
Bin Yuan ◽  
Dongliang Zhao ◽  
Yueqin Chen ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Communicating Hydrocephalus ◽  
Tgf Β1
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