Ultrastructural characterization of two new human endometrial carcinoma cell lines and normal human endometrial epithelial cells cultured on extracellular matrix

1990 ◽  
Vol 26 (7) ◽  
pp. 701-708 ◽  
Author(s):  
Jeff A. Boyd ◽  
Clifford A. Rinehart ◽  
Leslie A. Walton ◽  
Gene P. Siegal ◽  
David G. Kaufman
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Endometrial Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Ultrastructural Characterization ◽  
Normal Human ◽  
Endometrial Epithelial Cells ◽  
Cells Cultured

Characterization of human ovarian and endometrial carcinoma cell lines established on extracellular matrix

1989 ◽  
Vol 32 (2) ◽  
pp. 163-173 ◽  
Author(s):  
K. Crickard ◽  
M.J. Niedbala ◽  
U. Crickard ◽  
M. Yoonessi ◽  
A.A. Sandberg ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Endometrial Carcinoma Cell ◽  
Carcinoma Cell Lines

scholarly journals Characterization of Distinct Human Endometrial Carcinoma Cell Lines Deficient in Mismatch Repair That Originated from a Single Tumor

1998 ◽  
Vol 273 (41) ◽  
pp. 26662-26669 ◽  
Author(s):  
Warren E. Glaab ◽  
John I. Risinger ◽  
Asad Umar ◽  
Thomas A. Kunkel ◽  
J. Carl Barrett ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Mismatch Repair ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Endometrial Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Single Tumor

scholarly journals Characterization of genetically defined sporadic and hereditary type 1 papillary renal cell carcinoma cell lines

2021 ◽  
Author(s):  
Youfeng Yang ◽  
Christopher J. Ricketts ◽  
Cathy D. Vocke ◽  
J. Keith Killian ◽  
Hesed M. Padilla‐Nash ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Renal Cell ◽  
Carcinoma Cell ◽  
Papillary Renal Cell Carcinoma ◽  
Renal Cell Carcinoma Cell ◽  
Carcinoma Cell Lines

Cytometric Profiling of CD133+ Cells in Human Colon Carcinoma Cell Lines Identifies a Common core Phenotype and Cell Type-specific Mosaics

2013 ◽  
Vol 28 (3) ◽  
pp. 267-273 ◽  
Author(s):  
Marica Gemei ◽  
Rosa Di Noto ◽  
Peppino Mirabelli ◽  
Luigi Del Vecchio
Keyword(s):  
Colorectal Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Cell Type ◽  
Carcinoma Cell Lines ◽  
Cell Type Specific

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

Characterization of stem cell–like cancer cells in immune-competent mice

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3906-3912 ◽  
Author(s):  
Jorg A. Kruger ◽  
Charles D. Kaplan ◽  
Yunping Luo ◽  
He Zhou ◽  
Dorothy Markowitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Lines ◽  
Tumorigenic Potential ◽  
4T1 Cells

AbstractRecently, the cancer stem cell hypothesis has gained significant recognition as the descriptor of tumorigenesis. Although previous studies relied on transplanting human or rat tumor cells into immunecompromised mice, our study used the Hoechst 33342 dye–based side population (SP) technique to isolate and transplant stem cell–like cancer cells (SCLCCs) from the 4T1 and NXS2 murine carcinoma cell lines into the immune-competent microenvironment of syngeneic mice. 4T1 cells displayed an SP of 2% with a Sca-1highc-Kit–CD45– phenotype, whereas NXS2 cells contained an SP of 0.2% with a Sca-1highCD24highc-Kit–CD45–GD high2 phenotype. Reverse transcription–polymerase chain reaction (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-β2. Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxorubicin, which correlated with a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice. Most importantly, SP cells showed a markedly higher repopulation and tumorigenic potential in vivo, which correlated with an increased number of cells in the SP compartment of SP-derived tumors. Taken together, these results show that we successfully characterized SCLCCs from 2 murine carcinoma cell lines in the immune-competent microenvironment of syngeneic mice.

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