Short-term cultivation of murine thymic epithelial cells in a serum-free medium

1990 ◽  
Vol 26 (7) ◽  
pp. 671-681 ◽  
Author(s):  
Carsten Ropke ◽  
Bo Deurs ◽  
Ole W. Petersen
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Short Term ◽  
Serum Free

scholarly journals Human Thymic Epithelial Cells in Serum-Free Culture: Nature and Effects on Thymocyte Cell Lines

1992 ◽  
Vol 2 (2) ◽  
pp. 111-121 ◽  
Author(s):  
Carsten Ropke ◽  
Jette Elbroend
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Cell Types ◽  
Thymic Epithelial Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Factors ◽  
Serum Free ◽  
Functional Activities ◽  
Thymocyte Proliferation

Thymic epithelial cells (TEC) have been cultured for several months and/or for 4 to 5 transfers in a growth factor-defined serum-free medium without concurrent growth of other cell types. The use of monoclonal antibodies andαMAM-6 indicated that the majority of TEC were of medullary origin. The vast majority of cells were positive for LFA-3 and class I, and class II expression, was low or absent. Supernatants from the cultures were shown to contain IL-1ß, IL-6, and M-CSF. Coculture of cloned subpopulations of thymocytes and TEC showed effects of TEC and of secreted ILs on thymocyte proliferation. High percentages of TEC were able to bind DN, DP, or SP thymocyte populations, partly via CD2-LFA-3 adhesion. Thus, it is possible to culture TEC without unknown serum factors and with maintenance of functional activities.

Characterization of human thymic epithelial cells grown in serum-free medium

1984 ◽  
Vol 31 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Ilonna J. Rimm ◽  
Atul K. Bhan ◽  
Eveline E. Schneeberger ◽  
Stuart F. Schlossman ◽  
Ellis L. Reinherz
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology

1991 ◽  
Vol 125 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Bertil G. Casslén ◽  
Michael J. K. Harper
Keyword(s):  
Epithelial Cells ◽  
Primary Cultures ◽  
Collagen Matrix ◽  
Human Endometrium ◽  
Serum Free Medium ◽  
Free Medium ◽  
Endometrial Stromal Cells ◽  
Prolonged Incubation ◽  
Serum Free ◽  
Endometrial Epithelial Cells

Abstract. The aim of the study was to explore the possibility of using human endometrial epithelial cells in serum-free culture as a sensitive assay for hormonal effects on the human endometrium. Glands were isolated following enzymatic digestion of the endometrial tissue and plated on a collagen matrix. The epithelial cells were grown in either medium containing serum or in supplemented serum-free medium. No morphologic difference was found between cells grown in these two media for up to 5 days, using either light or scanning electron microscopy. Secretion of prostaglandin F2α (PGF2α) in response to estradiol was not lower in serum-free medium than in medium containing serum for the first 2 days of culture, whereas secretion declined after prolonged incubation in the serum-free medium. This response to estradiol was clearly dose-dependent, and it was further enhanced by addition of arachidonic acid, the precursor for prostaglandin synthesis, to the medium. Co-culture of endometrial stromal cells did not influence the secretion of PGF2α by epithelial cells. We conclude that the secretion of PGF2α from primary cultures of human endometrial epithelial cells grown on collagen in serum-free medium can be used for a limited period as an assay of estrogenic effects on the human endometrium.

Characterization of growth and differentiation of normal human submandibular gland epithelial cells in a serum-free medium

1993 ◽  
Vol 54 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Masaya Okura ◽  
Kanemitsu Shirasuna ◽  
Tsutomu Hiranuma ◽  
Hideo Yoshioka ◽  
Hirokazu Nakahara ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Submandibular Gland ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Normal Human

Growth of distal fetal rat lung epithelial cells in a defined serum-free medium

1991 ◽  
Vol 27 (8) ◽  
pp. 625-632 ◽  
Author(s):  
D. Jassal ◽  
R. N. N. Han ◽  
I. Caniggia ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Rat Lung ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Fetal Rat Lung

Growth of mouse endometrial luminal epithelial cells in vitro: functional integrity of the oestrogen receptor system and failure of oestrogen to induce proliferation

1991 ◽  
Vol 128 (1) ◽  
pp. 115-120 ◽  
Author(s):  
F.-D. A. Uchima ◽  
M. Edery ◽  
T. Iguchi ◽  
H. A. Bern
Keyword(s):  
Epithelial Cells ◽  
Oestrogen Receptor ◽  
Continuous Exposure ◽  
Oestrogen Receptors ◽  
Serum Free Medium ◽  
Free Medium ◽  
Receptor System ◽  
Serum Free ◽  
Progestin Receptors ◽  
Luminal Epithelial Cells

ABSTRACT Normal endometrial luminal epithelial cells isolated from ovariectomized approximately 40-day-old BALB/cCrgl mice were purified by Percoll density gradient centrifugation and grown as primary cultures in collagen gel matrix and serum-free medium. Cells increased threefold in number during the 9-day culture period. Deletion of insulin, epidermal growth factor or bovine serum albumin resulted in decreased growth. Addition of any single factor to the unsupplemented medium had no effect. Relatively high levels of cytosolic oestrogen receptors and progestin receptors were demonstrable in the cultures. Addition of oestrogen did not enhance epithelial cell proliferation. On the contrary, all doses of oestrogen (180 fmol/l to 218 nmol/l) were inhibitory. Continuous exposure to oestradiol-17β (1·8 nmol/l) for 9 days in serum-free medium resulted in a decrease in cytosolic oestrogen receptors with an associated nuclear accumulation of oestrogen receptors. A corresponding increase in cytosolic progestin receptors was also observed, indicating that no qualitative modification of the oestrogen receptor system had occurred. Thus, as previously reported for vaginal epithelial cells, oestrogen, despite its stimulation of specific product synthesis (progestin receptors), did not increase proliferation of endometrial luminal epithelial cells in this culture system. Journal of Endocrinology (1991) 128, 115–120

Isolation and culture of colonic epithelial cells in serum-free medium

1985 ◽  
Vol 9 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Ariane A. Thompson ◽  
Sonya Dilworth ◽  
Robert J. Hay
Keyword(s):  
Epithelial Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Colonic Epithelial Cells ◽  
Serum Free
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