Biocompatibility testing of an experimental fluoride releasing resin using human gingival epithelial cells in vitro

1989 ◽  
Vol 25 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Frederick H. Kasten ◽  
L. Fatima R. Pineda ◽  
Paul E. Schneider ◽  
H. Ralph Rawls ◽  
Theda A. Foster
Keyword(s):  
Epithelial Cells ◽  
Gingival Epithelial Cells ◽  
Biocompatibility Testing ◽  
Human Gingival Epithelial Cells

Effect of Inflammation on the Proliferation of Human Gingival Epithelial Cells In Vitro

1997 ◽  
Vol 68 (11) ◽  
pp. 1070-1075 ◽  
Author(s):  
Odile M. Carro ◽  
Suvia A. S. Evans ◽  
Cataldo W. Leone
Keyword(s):  
Epithelial Cells ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells

scholarly journals In Vitro Investigation of the Cytotoxic Effects of Different Detergent-Containing Children's Toothpastes on Human Gingival Epithelial Cells

2021 ◽  
Author(s):  
Sinem Birant ◽  
Yazgul Duran ◽  
Tunc Akkoc ◽  
Figen Seymen
Keyword(s):  
Epithelial Cells ◽  
Third Molar ◽  
Live Cell ◽  
Control Group ◽  
Dead Cell ◽  
Cytotoxic Effects ◽  
Gingival Epithelial Cells ◽  
In Vitro Investigation ◽  
Human Gingival Epithelial Cells

Abstract Background: This study aimed to evaluate possible cytotoxic effects to gingival epithelial cells exposed to children toothpastes containing different detergent. Methods: Tissues required fort he isolation of human gingival epithelial cells were obtained by biopsy during the extraction of the impacted third molar tooth. Toothpaste solutions of different concentrations were prepared from five different children’s toothpastes with different detergent contents. Isolated gingival epithelial cells were stimulated with experimental groups consisting of toothpaste solutions (Colgate, Sensodyne, Splat, Nenedent, Perlodent) at different concentrations and a control group consissting of complete Dulbocco’s modified eagle medium. After the experiments, cell viability was evaluated using flow cytometry. Data analysis were done using One Way ANOVA test and Tukey post-hoc test. Results: In all experimental groups, there was a decrease in live cell rates and an increase in dead cell rates due to increased concentration. The statistically highest live cell ratios were detected in Splat’s toothpaste solutions after the control group and the group with the lowest viability values was determined in Colgate group (p<0.05). Conclusions: According to the results of the study, it was observed that toothpastes containing SLS affected the viability of cells more negatively than toothpastes with other detergent contents.

scholarly journals Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells

2021 ◽  
Vol 22 (14) ◽  
pp. 7669
Author(s):  
Cassio Luiz Coutinho Almeida-da-Silva ◽  
Harmony Matshik Dakafay ◽  
Kaitlyn Liu ◽  
David M. Ojcius
Keyword(s):  
Epithelial Cells ◽  
Oral Cavity ◽  
Cigarette Smoke ◽  
Large Body ◽  
Receptor Expression ◽  
Host Cells ◽  
Dependent Manner ◽  
Gingival Epithelial Cells ◽  
Human Gingival Epithelial Cells ◽  
Oral Cells

A large body of evidence shows the harmful effects of cigarette smoke to oral and systemic health. More recently, a link between smoking and susceptibility to coronavirus disease 2019 (COVID-19) was proposed. COVID-19 is due to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which uses the receptor ACE2 and the protease TMPRSS2 for entry into host cells, thereby infecting cells of the respiratory tract and the oral cavity. Here, we examined the effects of cigarette smoke on the expression of SARS-CoV-2 receptors and infection in human gingival epithelial cells (GECs). We found that cigarette smoke condensates (CSC) upregulated ACE2 and TMPRSS2 expression in GECs, and that CSC activated aryl hydrocarbon receptor (AhR) signaling in the oral cells. ACE2 was known to mediate SARS-CoV-2 internalization, and we demonstrate that CSC treatment potentiated the internalization of SARS-CoV-2 pseudovirus in GECs in an AhR-dependent manner. AhR depletion using small interference RNA decreased SARS-CoV-2 pseudovirus internalization in CSC-treated GECs compared with control GECs. Our study reveals that cigarette smoke upregulates SARS-CoV-2 receptor expression and infection in oral cells. Understanding the mechanisms involved in SARS-CoV-2 infection in cells of the oral cavity may suggest therapeutic interventions for preventing viral infection and transmission.

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