Variable responsiveness of rat tracheal epithelial cells to bovine serum albumin in serum-free culture

1989 ◽  
Vol 25 (11) ◽  
pp. 1046-1050 ◽  
Author(s):  
David G. Thomassen
Keyword(s):  
Epithelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Free ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

Rat carcinoma cells in long-term, serum-free culture provide a continuing supply of collagenase

1985 ◽  
Vol 5 (12) ◽  
pp. 1071-1077 ◽  
Author(s):  
Geoffrey A. Stevenson ◽  
J. Guy Lyons ◽  
David A. Cameron ◽  
Robert L. O'Grady
Keyword(s):  
Epithelial Cells ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mammary Carcinoma ◽  
Bovine Serum ◽  
Defined Medium ◽  
Carcinoma Cells ◽  
Serum Free ◽  
Vertebrate Collagenase

Neoplastic, epithelial cells derived from a spontaneously-arising rat mammary carcinoma have been cultured in a defined medium, in the absence of serum, continuously, for over 2 years. The medium is a mixture of Ham's F12 and Dulbecco's Modified Eagle's media supplemented with insulin, transferrin and bovine serum albumin. The cells have retained their potential to produce tumours and, in culture, a true vertebrate collagenase. This system provides a continuing supply of vertebrate collagenase through the application of recently developed methods.

Serum-Free Culture of Mouse Tracheal Epithelial Cells

1997 ◽  
Vol 23 (5) ◽  
pp. 427-440 ◽  
Author(s):  
Rakesh K. Kumar ◽  
Sonia E. Maronese ◽  
Roslynn O'grady
Keyword(s):  
Epithelial Cells ◽  
Serum Free ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

scholarly journals Continuous culture of rat C6 glioma in serum-free medium.

1980 ◽  
Vol 87 (2) ◽  
pp. 434-441 ◽  
Author(s):  
R A Wolfe ◽  
G H Sato ◽  
D B McClure
Keyword(s):  
Growth Rate ◽  
Tissue Culture ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
C6 Glioma ◽  
Saturation Density ◽  
Serum Free

In this communication we describe serum-free culture conditions for the serial propagation of the C6 glioma cell line. The growth rate, saturation density, and morphology of these cells are equivalent to those of their serum-grown counterparts when cultured in a 3:1 mixture of Dulbecco's modified Eagle's medium and Ham's medium F-12 supplemented with trace elements, insulin, transferrin, fibroblast growth factor, linoleic acid complexed to fatty acid-free bovine serum albumin, and a serum-spreading factor (SSF) partially purified from human plasma. The requirement for SSF in the medium can be satisfied by preincubating the tissue culture dishes with SSF. Tissue culture dishes sequentially pretreated with poly-D-lysine and purified cold insouluble globulin will also substitute for this requirement. The fatty acid-free bovine serum albumin/linoleic acid complex increases the growth rate of these cells but has no appreciable effect on their morphology, saturation density, or ability to grow with repeated subculture. The growth stimulation caused by this complex appears to be dependent on the fatty acid, as the fatty acid-free bovine serum albumin alone has no effect on the growth rate. Linoleic acid is cytotoxic in the absence of bovine serum albumin, and the fatty acid-free bovine serum albumin prevents this toxicity. Other fatty acids including oleic, arachidonic, and palmitic only partially substitute for the growth-promoting effect of linoleic acid.

Accumulation and distribution of neutral lipid droplets is non-uniform in ovine blastocysts produced in vitro in either the presence or absence of serum

2005 ◽  
Vol 17 (8) ◽  
pp. 815 ◽  
Author(s):  
A. Reis ◽  
G. J. McCallum ◽  
T. G. McEvoy
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Neutral Lipid ◽  
Embryo Culture ◽  
Bovine Serum ◽  
Lipid Droplets ◽  
Serum Free ◽  
Oviduct Fluid ◽  
Abundance And Distribution

Sheep zygotes were cultured in serum-free or serum-supplemented media to determine effects on blastocyst yields and within-blastocyst abundance and distribution of neutral lipid droplets. Embryos cultured in synthetic oviduct fluid supplemented with bovine serum albumin (0.4% w/v) (SBSA) generated similar blastocyst yields (mean ± s.e.m. = 20% ± 5) to those in synthetic oviduct fluid supplemented with serum (10% v/v) from ewes fed a diet containing 0% (SZFO; 26% ± 2) or 3% fish oil (S3FO; 23% ± 3). SBSA zygotes generated more good-quality blastocysts than their SZFO or S3FO counterparts (P < 0.05). Within-blastocyst abundance of neutral lipid droplets was non-uniform; data were collected from discrete embryo sectors (each = 2700 µm2) representing highest (H), intermediate (I) and lowest (L) densities of accumulation. For all sectors, area (µm2) occupied by lipid droplets in SBSA blastocysts (mean H = 470; I = 370; L = 245) was smaller (P < 0.01) than occupied in others (SBSA : SZFO = 1 : 1.41, 1 : 1.48 and 1 : 1.42; SBSA : S3FO = 1 : 1.36, 1 : 1.30 and 1 : 1.31; data for H, I and L, respectively). Among S3FO blastocysts only, inferior quality was associated with greater lipid abundance. Overall, embryo culture in the presence of serum increased neutral lipid droplet abundance but accumulation was non-uniform.

scholarly journals The effects on cell adhesion of fibronectin and gelatin in a serum-free, bovine serum albumin medium.

1982 ◽  
Vol 7 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Mikio Kan ◽  
Yoshiki Minamoto ◽  
Sachiko Sunami ◽  
Isao Yamam ◽  
Makoto Umeda
Keyword(s):  
Cell Adhesion ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Serum Free
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