Growth inhibitory effects of dimethyl sulfoxide and dimethyl sulfone on vascular smooth muscle and endothelial cells in vitro

1987 ◽  
Vol 23 (6) ◽  
pp. 422-428 ◽  
Author(s):  
Don L. Layman
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Dimethyl Sulfoxide ◽  
Inhibitory Effects ◽  
Dimethyl Sulfone ◽  
Growth Inhibitory

scholarly journals Production of proteolytic enzymes in mast cells, fibroblasts, vascular smooth muscle and endothelial cells cultivated under normoxic or hypoxic conditions

2010 ◽  
pp. 711-719 ◽  
Author(s):  
H Maxová ◽  
L Bačáková ◽  
V Lisá ◽  
J Novotná ◽  
H Tomášová ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Mast Cells ◽  
Vascular Smooth Muscle ◽  
Serine Proteases ◽  
Proteolytic Enzymes ◽  
Cell Types ◽  
In Vitro Production ◽  
Hypoxic Conditions

Matrix metalloproteinases (MMPs) is a family of proteolytic enzymes involved in remodeling of extracellular matrix. Although proteolytic enzymes are produced by many cell types, mast cells seem to be more important than other types in remodeling of pulmonary arteries during hypoxia. Therefore, we tested in vitro production of MMPs and serine proteases in four cell types (mast cells, fibroblasts, vascular smooth muscle cells and endothelial cells) cultivated for 48 h under normoxic or hypoxic (3 % O2) conditions. MMP-13 was visualized by immunohistochemistry, MMP-2 and MMP-9 were detected by zymography in cell lysates. Enzymatic activities (MMPs, tryptase and chymase) were estimated in the cultivation media. Hypoxia had a minimal effect on total MMP activity in the cultivation media of all types of cells, but immunofluorescence revealed higher intensity of MMP-13 in the cells exposed to hypoxia except of fibroblasts. Tryptase activity was three times higher and chymase activity twice higher in mast cells cultivated in hypoxia than in those cultured in normoxia. Among all cell types studied here, mast cells are the most abundant source of proteolytic enzymes under normoxic and hypoxic conditions. Moreover, in these cells hypoxia increases the production of both specific serine proteases tryptase and chymase, which can act as MMPs activators.

Preparation of Arsenic Trioxide-Loaded PLGA Nanoparticles and Investigation of its Inhibitory Effects on Proliferation of Rabbit Vascular Smooth Muscle Cells In Vitro

2009 ◽  
Vol 60-61 ◽  
pp. 125-129
Author(s):  
Su Su Zhao ◽  
Qin Lu ◽  
Dong Sheng Zhang
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Arsenic Trioxide ◽  
Vascular Smooth Muscle Cells ◽  
Drug Loading ◽  
Muscle Cells ◽  
Plga Nanoparticles ◽  
Inhibitory Effects

Proliferation and migration of vascular smooth muscle cells(VSMCs) into the sub-intimal space play an important role in intimal thickening in atherosclerosis and in-stent restenosis. Arsenic compounds are natural substances that have been used in the treatment of patients with acute promyelocytic leukemia (APL).And Arsenic trioxide (As2O3) is known to be a potent inhibitor of cell proliferation.The aim of this study was to prepare arsenic trioxide (As2O3) -loaded PLGA nanoparticles(As2O3-PLGA-NP) and investigate its general properties, preservation of As2O3 bioactivity and their inhibitory effects on Rabbit Vascular Smooth Muscle Cells(RVSMCs) in vitro. With PLGA as carrier,As2O3 drug delivery nanoparticles were prepared by w /o /w double-emulsion evaporation technique，and their inhibitory effects on Rabbit Vascular Smooth Muscle Cells(VSMCs) in vitro was investigated.The results of the experiment show the self-prepared As2O3-PLGA-NP were approximately spherical,with the mean diameter of 90±25.03 nm, and the average drug loading was 1.72% .The As2O3-PLGA-NP has drug sustained-release character and can prolong the phase of the inhibitory effect of As2O3 against RVSMCs.The As2O3-PLGA-NP do not reduce the biological activity of As2O3.

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