Comparison of trout hepatocyte culture on different substrates

1986 ◽  
Vol 22 (6) ◽  
pp. 360-362 ◽  
Author(s):  
Michael M. Lipsky ◽  
Talia R. Sheridan ◽  
Richard O. Bennett ◽  
Eric B. May
Keyword(s):  
Hepatocyte Culture ◽  
Trout Hepatocyte

Trout hepatocyte culture: Isolation and primary culture

In Vitro ◽  
1985 ◽  
Vol 21 (4) ◽  
pp. 221-228 ◽  
Author(s):  
James E. Klaunig ◽  
Randall J. Ruch ◽  
Peter J. Goldblatt
Keyword(s):  
Primary Culture ◽  
Hepatocyte Culture ◽  
Trout Hepatocyte

PARALLEL OPERATION OF TWO SLIDEREACTOR BIOREACTOR SYSTEMS – A TOOL FOR HYPOTHESIS DRIVEN HEPATOCYTE CULTURE EXPERIMENTS

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
N Billecke ◽  
N Raschzok ◽  
H Morgul ◽  
C Leist ◽  
K Schmitt ◽  
...  
Keyword(s):  
Hepatocyte Culture ◽  
Parallel Operation

A rational design approach for amino acid supplementation in hepatocyte culture

2009 ◽  
Vol 103 (6) ◽  
pp. 1176-1191 ◽  
Author(s):  
Hong Yang ◽  
Charles M. Roth ◽  
Marianthi G. Ierapetritou
Keyword(s):  
Amino Acid ◽  
Rational Design ◽  
Design Approach ◽  
Hepatocyte Culture ◽  
Amino Acid Supplementation ◽  
Acid Supplementation

scholarly journals Polyanion-induced, microfluidic engineering of fragmented collagen microfibers for reconstituting extracellular environments of 3D hepatocyte culture

2021 ◽  
pp. 112417
Author(s):  
Rie Utoh ◽  
Sakiko Enomoto ◽  
Masumi Yamada ◽  
Keigo Yamanaka ◽  
Yuya Yajima ◽  
...  
Keyword(s):  
Hepatocyte Culture

Evidence for the role of a Na(+)/HCO(3)(−) cotransporter in trout hepatocyte pHi regulation

2000 ◽  
Vol 203 (14) ◽  
pp. 2201-2208 ◽  
Author(s):  
M. Furimsky ◽  
T.W. Moon ◽  
S.F. Perry
Keyword(s):  
Membrane Potential ◽  
Anion Transport ◽  
Fluorescent Dye ◽  
Proton Extrusion ◽  
Pharmacological Agents ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Trout Hepatocyte ◽  
Acid Loading ◽  
Na Uptake ◽  
Phi Regulation

The mechanisms of intracellular pH (pHi) regulation were examined in hepatocytes of the rainbow trout Oncorhynchus mykiss. pHi was monitored using the pH-sensitive fluorescent dye BCECF, and the effects of various media and pharmacological agents were examined for their influence on baseline pHi and recovery rates from acid and base loading. Rates of Na(+) uptake were measured using (22)Na, and changes in membrane potential were examined using the potentiometric fluorescent dye Oxonol VI. The rate of proton extrusion following acid loading was diminished by the blockade of either Na(+)/H(+) exchange (using amiloride) or anion transport (using DIDS). The removal of external HCO(3)(−) and the abolition of outward K(+) diffusion by the channel blocker Ba(2+) also decreased the rate of proton extrusion following acid load. Depolarization of the cell membrane with 50 mmol l(−)(1) K(+), however, did not affect pHi. The rate of recovery from base loading was significantly diminished by the blockade of anion transport, removal of external HCO(3)(−) and, to a lesser extent, by blocking Na(+)/H(+) exchange. The blockade of K(+) conductance had no effect. The decrease in Na(+) uptake rate observed in the presence of the anion transport blocker DIDS and the DIDS-sensitive hyperpolarization of membrane potential during recovery from acid loading suggest that a Na(+)-dependent electrogenic transport system is involved in the restoration of pHi after intracellular acidification. The effects on baseline pHi indicate that the different membrane exchangers are tonically active in the maintenance of steady-state pHi. This study confirms the roles of a Na(+)/H(+) exchanger and a Cl(−)/HCO(3)(−) exchanger in the regulation of trout hepatocyte pHi and provides new evidence that a Na(+)/HCO(3)(−) cotransporter contributes to pHi regulation.

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