Human dermal microvascular endothelial cells: An improved method for tissue culture and a description of some singular properties in culture

1985 ◽  
Vol 21 (11) ◽  
pp. 627-635 ◽  
Author(s):  
R. M. Marks ◽  
M. Czerniecki ◽  
R. Penny
Keyword(s):  
Tissue Culture ◽  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Improved Method ◽  
Microvascular Endothelial

scholarly journals An improved method for isolation of microvascular endothelial cells from normal and inflamed human lung

1998 ◽  
Vol 34 (7) ◽  
pp. 529-536 ◽  
Author(s):  
Jin Ning Lou ◽  
Nabil Mili ◽  
Christine Decrind ◽  
Yves Donati ◽  
Sylvie Kossodo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Improved Method ◽  
Microvascular Endothelial

Resveratrol Reduces Matrix Metalloproteinase-2 Activity Induced by Oxygen-Glucose Deprivation and Reoxygenation in Human Cerebral Microvascular Endothelial Cells

2012 ◽  
Vol 82 (4) ◽  
pp. 267-274 ◽  
Author(s):  
Zahide Cavdar ◽  
Mehtap Y. Egrilmez ◽  
Zekiye S. Altun ◽  
Nur Arslan ◽  
Nilgun Yener ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Neurovascular Unit ◽  
Glucose Deprivation ◽  
Matrix Metalloproteinase 2 ◽  
Microvascular Endothelial Cells ◽  
Oxygen Glucose Deprivation ◽  
Microvascular Endothelial

The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Among the human matrix metalloproteinases (MMPs), MMP-2 and -9, known as gelatinases, are the key enzymes for degrading type IV collagen, which is the major component of the basal membrane that surrounds the cerebral blood vessel. In the present study, we investigated the effects of resveratrol on cytotoxicity, reactive oxygen species (ROS), and gelatinases (MMP-2 and -9) in human cerebral microvascular endothelial cells exposed to 6 hours of oxygen-glucose deprivation and a subsequent 24 hours of reoxygenation with glucose (OGD/R), to mimic ischemia/reperfusion in vivo. Lactate dehydrogenase increased significantly, in comparison to that in the normoxia group. ROS was markedly increased in the OGD/R group, compared to normoxia. Correspondingly, ROS was significantly reduced with 50 μM of resveratrol. The proMMP-2 activity in the OGD/R group showed a statistically significant increase from the control cells. Resveratrol preconditioning decreased significantly the proMMP-2 in the cells exposed to OGD/R in comparison to that in the OGD/R group. Our results indicate that resveratrol regulates MMP-2 activity induced by OGD/R via its antioxidant effect, implying a possible mechanism related to the neuroprotective effect of resveratrol.

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