Fibroblast growth factor induces the soft agar growth of two non-transformed celllines

1986 ◽  
Vol 22 (12) ◽  
pp. 749-755 ◽  
Author(s):  
Angie Rizzino ◽  
Eric Ruff
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Soft Agar ◽  
Fibroblast Growth ◽  
Soft Agar Growth

scholarly journals Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA.

1988 ◽  
Vol 8 (2) ◽  
pp. 588-594 ◽  
Author(s):  
R Sasada ◽  
T Kurokawa ◽  
M Iwane ◽  
K Igarashi
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cellular Transformation ◽  
Soft Agar ◽  
Transformed Cells ◽  
Fibroblast Growth ◽  
Morphological Transformation ◽  
Phenotypic Alteration ◽  
Autocrine Stimulation

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.

scholarly journals Expression of human basic fibroblast growth factor cDNA in baby hamster kidney-derived cells results in autonomous cell growth.

1988 ◽  
Vol 106 (4) ◽  
pp. 1385-1394 ◽  
Author(s):  
G Neufeld ◽  
R Mitchell ◽  
P Ponte ◽  
D Gospodarowicz
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Constitutive Expression ◽  
Human Tumor ◽  
Soft Agar ◽  
Biologically Active ◽  
Fibroblast Growth ◽  
Baby Hamster Kidney ◽  
High Concentration

Growth factor over-production by responsive cells might contribute to their autonomous proliferation as well as their acquisition of a transformed phenotype in culture. Basic fibroblast growth factor (bFGF) has been shown to induce transient changes in cell behavior that resemble those encountered in transformed cells. In addition, several types of human tumor cells have been shown to produce bFGF. To determine directly the role that bFGF might play in the induction of the transformed phenotype, we have introduced a human bFGF cDNA expression vector into baby hamster kidney-derived (BHK-21) fibroblasts. One of the BHK transfectants, termed clone 19, expresses the bFGF mRNA and produces biologically active bFGF that accumulates to a high concentration inside the cells. These properties correlate with the ability of the cells to grow in serum-free medium without the addition of exogenous bFGF. Clone 19 cells also proliferated in soft agar, indicating that constitutive expression of the bFGF gene results in a loss of anchorage-dependent growth.

scholarly journals The Use of Human Serum Samples to Study Malignant Transformation: A Pilot Study

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2670
Author(s):  
Andreana N. Holowatyj ◽  
Biljana Gigic ◽  
Christy A. Warby ◽  
Jennifer Ose ◽  
Tengda Lin ◽  
...  
Keyword(s):  
Pilot Study ◽  
Growth Factor ◽  
Human Serum ◽  
Fibroblast Growth Factor ◽  
Malignant Transformation ◽  
Biomarker Discovery ◽  
Soft Agar ◽  
Fibroblast Growth ◽  
Serum Factors ◽  
Men And Women

Obesity and excess adiposity account for approximately 20% of all cancer cases; however, biomarkers of risk remain to be elucidated. While fibroblast growth factor-2 (FGF2) is emerging as an attractive candidate biomarker for visceral adipose tissue mass, the role of circulating FGF2 in malignant transformation remains unknown. Moreover, functional assays for biomarker discovery are limited. We sought to determine if human serum could stimulate the 3D growth of a non-tumorigenic cell line. This type of anchorage-independent 3D growth in soft agar is a surrogate marker for acquired tumorigenicity of cell lines. We found that human serum from cancer-free men and women has the potential to stimulate growth in soft agar of non-tumorigenic epithelial JB6 P+ cells. We examined circulating levels of FGF2 in humans in malignant transformation in vitro in a pilot study of n = 33 men and women. Serum FGF2 levels were not associated with colony formation in epithelial cells (r = 0.05, p = 0.80); however, a fibroblast growth factor receptor-1 (FGFR1) selective inhibitor significantly blocked serum-stimulated transformation, suggesting that FGF2 activation of FGFR1 may be necessary, but not sufficient for the transforming effects of human serum. This pilot study indicates that the FGF2/FGFR1 axis plays a role in JB6 P+ malignant transformation and describes an assay to determine critical serum factors that have the potential to promote tumorigenesis.

Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA

1988 ◽  
Vol 8 (2) ◽  
pp. 588-594
Author(s):  
R Sasada ◽  
T Kurokawa ◽  
M Iwane ◽  
K Igarashi
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cellular Transformation ◽  
Soft Agar ◽  
Transformed Cells ◽  
Fibroblast Growth ◽  
Morphological Transformation ◽  
Phenotypic Alteration ◽  
Autocrine Stimulation

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.

scholarly journals Potential oncogenic effects of basic fibroblast growth factor requires cooperation between CUG and AUG-initiated forms.

1991 ◽  
Vol 2 (9) ◽  
pp. 709-718 ◽  
Author(s):  
B Couderc ◽  
H Prats ◽  
F Bayard ◽  
F Amalric
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Constitutive Expression ◽  
Soft Agar ◽  
Fibroblast Growth ◽  
Aortic Endothelial Cells ◽  
Tumorigenic Potential ◽  
Cell Immortalization

Normal adult bovine aortic endothelial cells were infected with various recombinant retroviruses expressing one, two, or three human basic fibroblast growth factor (bFGF) proteins normally synthesized by an alternative use of translation initiation codons. We show here that the constitutive expression of the AUG-initiated from (18 kDa) leads the transfected cells to form colonies in soft agar. The expression of the high molar weight (HMW) forms (22.5 and 21 kDa) initiated at one of the two CUG initiation codons allows cell immortalization, whereas the tumorigenic potential is reached when the three forms are constitutively expressed. Furthermore, we provide evidence that constitutive expression of (HMW) bFGF forms has a down-regulation effect on bFGF synthesis from the gene naturally active in parental endothelial cells.

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