Comparative measurements of potassium and chloride with ion-sensitive microelectrodes and x-ray microanalysis in cultured skeletal muscle fibers

In Vitro ◽  
1985 ◽  
Vol 21 (1) ◽  
pp. 45-48 ◽  
Author(s):  
H. Acker ◽  
F. Pietruschka ◽  
K. Zierold
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
X Ray ◽  
Skeletal Muscle Fibers ◽  
Ion Sensitive Microelectrodes

scholarly journals Backward Movements of Cross-Bridges by Application of Stretch and by Binding of MgADP to Skeletal Muscle Fibers in the Rigor State as Studied by X-Ray Diffraction

1999 ◽  
Vol 76 (4) ◽  
pp. 1770-1783 ◽  
Author(s):  
Yasunori Takezawa ◽  
Duck-Sool Kim ◽  
Masaki Ogino ◽  
Yasunobu Sugimoto ◽  
Takakazu Kobayashi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
X Ray Diffraction ◽  
X Ray ◽  
Rigor State ◽  
Skeletal Muscle Fibers ◽  
Cross Bridges

scholarly journals Sarcomere-Length Dependence of the Low Angle X-Ray Pattern from Skeletal Muscle Fibers at Rest and during Isometric Contraction

2012 ◽  
Vol 102 (3) ◽  
pp. 147a-148a
Author(s):  
Gabriella Piazzesi ◽  
Massimo Reconditi ◽  
Elisabetta Brunello ◽  
Luca Fusi ◽  
Marco Linari ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Isometric Contraction ◽  
Sarcomere Length ◽  
Muscle Fibers ◽  
X Ray ◽  
Length Dependence ◽  
Skeletal Muscle Fibers

Quantitative x-ray elemental imaging in stimulated single intact skeletal muscle fibers: The fate of calcium in the presence of ryanodine

1988 ◽  
Vol 46 ◽  
pp. 90-91
Author(s):  
J. Sommer ◽  
P. Ingram ◽  
A. LeFurgey ◽  
R. Nassar ◽  
T. High
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Time Course ◽  
Imaging System ◽  
Quantitative Imaging ◽  
Muscle Fibers ◽  
Frog Skeletal Muscle ◽  
X Ray ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried

We are involved in a continuing series of experiments aimed at a complete description,in terms of morphology and quantitative topochemistry, of the time course of spatial distributions of physiologically important elements during excitation-contraction coupling (ECC) at different time intervals (fractions of msec) following electrical stimulation of single, intact frog skeletal muscle fibers. In this present study wg report such distributions for Ca after 1,2 and 3 min of electrical stimulation in the presence of 2x10-4 M ryanodine, an alkaloid that, in time, causes irreversible muscle contractures.Single, intact frog skeletal muscle fibers were quick-frozen, cryosectioned, freeze-substituted and in one case freeze-fractured. The freeze-dried cryosections were subjected to electron probe X-ray microanalysis (EPXMA) in a JEOL 1200EX analytical electron microscope equipped with a Tracor Northern X-ray detector and a fully quantitative imaging system. Both, 64/64 pixel images (ambient temp.), and small raster probes (cold stage,-115 °C) for better statistics, were obtained, each from the same section.

scholarly journals X-ray diffraction from skeletal muscle fibers with diffused Dictyostelium G680V myosin subfragment-1

2003 ◽  
Vol 43 (supplement) ◽  
pp. S129
Author(s):  
H. Iwamoto ◽  
T. Uyeda ◽  
E. Katayama ◽  
J. Wakayama ◽  
T. Tamura ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
X Ray Diffraction ◽  
X Ray ◽  
Skeletal Muscle Fibers ◽  
Myosin Subfragment ◽  
Myosin Subfragment 1

scholarly journals Dynamics of Thin-Filament Activation in Rabbit Skeletal Muscle Fibers Examined by Time-Resolved X-Ray Diffraction

2009 ◽  
Vol 96 (3) ◽  
pp. 1045-1055 ◽  
Author(s):  
Takumi Tamura ◽  
Jun'ichi Wakayama ◽  
Katsuaki Inoue ◽  
Naoto Yagi ◽  
Hiroyuki Iwamoto
Keyword(s):  
Skeletal Muscle ◽  
Thin Filament ◽  
Muscle Fibers ◽  
Rabbit Skeletal Muscle ◽  
X Ray Diffraction ◽  
Time Resolved ◽  
X Ray ◽  
Skeletal Muscle Fibers ◽  
Filament Activation

Quantitative elemental x-ray imaging of timed calcium displacement from stores (JSR) in isolated single frog skeletal muscle fibers: Its efficacy in quantitation

1989 ◽  
Vol 47 ◽  
pp. 240-241 ◽  
Author(s):  
R. Nassar ◽  
P. Ingram ◽  
T. High ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Time Course ◽  
Muscle Fibers ◽  
X Ray ◽  
Statistical Confidence ◽  
Scanning Time ◽  
Skeletal Muscle Fibers ◽  
X Ray Imaging ◽  
Series Of Experiments ◽  
Spatial Displacements

We are performing a continuing series of experiments to describe the time course of fast physiological events in terms of morphology and microtopochemistry, using electron probe x-ray microanalysis in both the static probe (STP) and quantitative digital imaging (QDI) modes. As a model, we are using timed spatial displacements of elements (e.g. the release of calcium from JSR) during the process of excitation-contraction coupling in single, intact skeletal muscle fibers quick-frozen at known time intervals following electrical stimulation. There is considerable variance in the total calcium concentration ([Ca]t) among JSRs, which increases the requirement for widespread sampling to increase statistical confidence. Even at a low number of pixels/raster chosen for time economy, QDI seems well suited to deal with this variance because it covers a large number of JSRs in a reasonably short scanning time (64x64 pixels: ∽3 h; 128×128 pixels: ∽9 h). Here, we report on the efficacy of QDI in our experiments and compare the results with those obtained from STP.

scholarly journals Origin of X-ray reflections from skeletal muscle fibers as examined by contrast variation.

2001 ◽  
Vol 41 (supplement) ◽  
pp. S49
Author(s):  
J. Wakayama ◽  
T. Fujisawa ◽  
H. Iwamoto
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Contrast Variation ◽  
X Ray ◽  
Skeletal Muscle Fibers
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