Polarographic measurement of oxygen uptake by astrocytes in primary cultures using the tissue-culture flask as the respirometer chamber

In Vitro ◽  
1979 ◽  
Vol 15 (6) ◽  
pp. 429-436 ◽  
Author(s):  
Elna Hertz ◽  
Leif Hertz
Keyword(s):  
Tissue Culture ◽  
Oxygen Uptake ◽  
Primary Cultures ◽  
Culture Flask ◽  
Tissue Culture Flask ◽  
Polarographic Measurement

Automated Application of a Novel High Yield, High Performance Tissue Culture Flask

2008 ◽  
Vol 13 (3) ◽  
pp. 136-144
Author(s):  
S SZYMANSKI ◽  
K HUFF ◽  
A PATEL ◽  
J MURRAY ◽  
J FEASBY ◽  
...  
Keyword(s):  
Tissue Culture ◽  
High Performance ◽  
High Yield ◽  
Culture Flask ◽  
Tissue Culture Flask

A new tissue culture flask with demountable bottom

1958 ◽  
Vol 14 (1) ◽  
pp. 219-221 ◽  
Author(s):  
A.C. Pipkin ◽  
A.D. Mack
Keyword(s):  
Tissue Culture ◽  
Culture Flask ◽  
Tissue Culture Flask

In vitro cloning of animal-infective bloodstream forms of Trypanosoma brucei

Parasitology ◽  
1980 ◽  
Vol 80 (2) ◽  
pp. 371-382 ◽  
Author(s):  
H. Hirumi ◽  
K. Hirumi ◽  
J. J. Doyle ◽  
G. A. M. Cross
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Brucei ◽  
Fresh Medium ◽  
Population Doubling ◽  
Feeder Cells ◽  
Culture Flask ◽  
Single Organism ◽  
Variable Antigen ◽  
Doubling Times

SummaryClones of animal-infective bloodstream forms of Trypanosoma brucei (stocks S.427 and LUMP 227) were made by transferring a single organism from bloodstream-form cultures into each well of Microtest II Tissue Culture Plates containing bovine fibroblast-like feeder cells. When the number of trypanosomes increased to 102–103/well on days 4–16, they were transferred into plastic T-25 culture flasks also containing feeder cells and fresh medium. Cultures were thereafter maintained by partially replacing the trypanosome suspension with the same volume of fresh medium (diluting the density to 2–5 × 105 trypanosomes/ml) every 24 h. Sub-cultivations could be made by transferring 1–2 ml of the trypanosome suspension to a new culture flask at 4–5 day intervals. A total of 42 clones in the 3 series TC221, TC52 and TC227, carrying variable antigen types (VATs) 221, 052 and ILTat 1·4, respectively, have been established. Average population doubling times for clones of TC221, TC52 and TC227 were 8·7, 14·5 and 15·5 h respectively. Of 35 populations examined, 34 clones retained the original specificity of their VATs for at least 8–32 days from cloning. One cloned population of TC52 consisted of 99·8% VAT 052 and 0·2% VAT 221 at the time when the first VAT test was made on day 18.

In vitro cultivation of hemocytes of Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae)

1971 ◽  
Vol 49 (10) ◽  
pp. 1355-1358 ◽  
Author(s):  
S. S. Sohi
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Primary Cultures ◽  
Malacosoma Disstria ◽  
In Vitro Cultivation ◽  
Original Source ◽  
Insect Tissue Culture ◽  
Fetal Bovine

Prolonged culturing of the hemocytes of Malacosoma disstria has been accomplished using Grace's insect tissue culture medium supplemented with fetal bovine serum (5%) and Bombyx mori hemolymph (3%). The cultures started to grow after 3–6 months. These cells have now been in vitro for over 16 months, and have been subcultured 35 times. Three types of cells were present in primary cultures, but only one type, prohemocytes, persisted and grew after subculturing. The M. disstria larvae that were used as the original source of hemocytes were naturally infected with the microsporidian Glugea disstriae. The microsporidian also grew in [he cell cultures, and the cells are still infected.

PEMBUATAN KULTUR SEL PRIMER DARI SIRIP EKOR IKAN MAS (Cyprinus carpio)

2009 ◽  
Vol 4 (1) ◽  
pp. 107
Author(s):  
Tuti Sumiati ◽  
Lila Gardenia ◽  
Agus Sunarto
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Primary Cell Culture ◽  
Fetal Bovine Serum ◽  
Primary Cell ◽  
Culture Flask ◽  
Kanamycin Sulfate ◽  
Common Carp Cyprinus Carpio ◽  
Fetal Bovine ◽  
Tissue Culture Flask

Tujuan dari penelitian ini adalah untuk membuat kultur sel primer dari sirip ekor ikan mas (Cyprinus carpio) dan diberi nama common carp tail (CCT). Explant ditumbuhkan dalam cawan kultur (culture flask) ukuran 25 cm2 yang berisi media Leibovitz’s L-15 dengan penambahan serum 20%, Penicillin 250 IU, Streptomycin 250 µg/mL, Kanamycin Sulfate 250 µg/mL dan L-Glutamin 2 mM, serta diinkubasi pada suhu 28oC. Perbedaan perlakuan berupa waktu pergantian media dan konsentrasi media dilakukan untuk mendapatkan kultur sel primer. Hasil pengamatan menunjukkan bahwa explant menunjukkan pertumbuhan sel setelah diinkubasi selama 24 jam. Pembentukan sel selapis (monolayer) mulai terlihat pada hari ke-4. Pasase pertama dilakukan pada hari ke-21 saat konfluensi mencapai 65%. Pasase selanjutnya dilakukan setiap 3 minggu di mana konfluensi mencapai 70%-80%. CCT terdiri atas sel berbentuk fibroblas dan epitel, dan berhasil dipasase sebanyak 12 kali selama lebih 2 tahun pemeliharaan.The objectives of this research were to develop primary cell from caudal fin of common carp (Cyprinus carpio) and designate it as Common Carp Tail (CCT). The explants were maintained in 25 cm2 tissue culture flask containing Leibovitz’s L-15 medium supplemented with 20% Fetal bovine serum, 250IU Penicilline, 250 µg/mL Streptomycin, 250 µg/mL Kanamycin Sulphate and 2 mM L-Glutamin, and incubated at 28oC. Different concentrations of glutamine and media, and timing of media replacement were applied to establish primary cell culture. The result showed that explants produced cell outgrowth after 24 hours of incubation. Monolayer was first observed at day 4th. First passage was done at day 21st when the cells achieved 65% confluent. The subsequence passages were done every 3 weeks when the cells reached 70-80% confluent. CCT consisted of both fibroblast-like and epithelial-like cells, and has been passaged for 12 times for over 2 years. 

scholarly journals Synthesis and regulation of acute phase plasma proteins in primary cultures of mouse hepatocytes.

1983 ◽  
Vol 97 (3) ◽  
pp. 866-876 ◽  
Author(s):  
H Baumann ◽  
G P Jahreis ◽  
K C Gaines
Keyword(s):  
Tissue Culture ◽  
Acute Phase ◽  
Plasma Proteins ◽  
Intracellular Transport ◽  
Response Spectrum ◽  
Primary Cultures ◽  
Acute Phase Reaction ◽  
Phase Reaction ◽  
Amyloid A ◽  
Mouse Hepatocytes

Adult mouse hepatocytes respond in vivo to experimentally induced acute inflammation by an increased synthesis and secretion of alpha 1-acid glycoprotein, haptoglobin, hemopexin, and serum amyloid A. Concurrently, the production of albumin and apolipoprotein A-1 is reduced. To define possible mediators of this response and to study their action in tissue culture, we established primary cultures of hepatocytes. Various hormones and factors that have been proposed to regulate the hepatic acute phase reaction were tested for their ability to modulate the expression of plasma proteins in these cells. Acute phase plasma and conditioned medium from activated monocytes influenced the production of most acute phase plasma proteins, and the regulation appears to occur at the level of functional mRNA. Purified hormones produced a significant anabolic response in only a few cases: dexamethasone was found to be effective in maintaining differentiated expression of the cells; and glucagon produced a specific inhibition of haptoglobin synthesis. When cells were treated with a combination of conditioned monocyte medium and dexamethasone, secretion of proteins was markedly reduced. The carbohydrate moieties of all plasma glycoproteins were incompletely modified, apparently as a result of decreased intracellular transport of newly synthesized plasma proteins. Although primary hepatocytes were not phenotypically stable in tissue culture, the cells nevertheless retained a broad response spectrum to exogenous signals. We propose this as a useful system to study the production of plasma proteins and thereby pinpoint the nature and activity of effectors mediating the hepatic acute phase reaction.

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