Isolation and characterization of diploid clones from adult and newborn rat liver cell lines

In Vitro ◽  
1979 ◽  
Vol 15 (6) ◽  
pp. 393-400 ◽  
Author(s):  
Takayoshi Tokiwa ◽  
Hidekazu Nakabayashi ◽  
Masahiro Miyazaki ◽  
Jiro Sato
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Newborn Rat ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a mannan-binding protein from rat liver.

1981 ◽  
Vol 256 (9) ◽  
pp. 4247-4252
Author(s):  
Y. Mizuno ◽  
Y. Kozutsumi ◽  
T. Kawasaki ◽  
I. Yamashina
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Isolation And Characterization

Isolation and characterization of conditionally immortalized astrocyte cell lines derived from adult human spinal cord

Glia ◽  
1994 ◽  
Vol 10 (3) ◽  
pp. 211-226 ◽  
Author(s):  
Scott R. Whittemore ◽  
Joseph T. Neary ◽  
Naomi Kleitman ◽  
Henry R. Sanon ◽  
Adelaida Benigno ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cell Lines ◽  
Human Spinal Cord ◽  
Adult Human ◽  
Isolation And Characterization

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

1978 ◽  
Vol 234 (3) ◽  
pp. C122-C130 ◽  
Author(s):  
D. M. Bissell ◽  
G. A. Levine ◽  
M. J. Bissell
Keyword(s):  
Glucose Metabolism ◽  
Primary Culture ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Time Course ◽  
Primary Cultures ◽  
Functional Change ◽  
Permanent Cell ◽  
Rat Hepatoma

The metabolic fate of [U-14C]glucose has been examined in detail in adult rat hepatocytes in primary monolayer culture, as well as in two permanent cell lines--Buffalo rat liver (BRL) and transplantable rat hepatoma (HTC) cells-derived from normal rat liver and from rat hepatoma, respectively. Under defined conditions of incubation, at a glucose concentration of 5.5 mM, the three types of cultured liver cells exhibited pronounced differences in glucose metabolism. Primary cultures, like the intact liver, differed from the cell lines in consuming relatively small amounts of glucose and converting approximately 50% of the total metabolized glucose to lactate. By contrast, the permantent cell lines consumed glucose at a 40-fold greater rate than did primary cultures, converting 80--90% of the carbohydrate to lactate. Oxidative metabolism of glucose carbon also differed among the three types of liver culture. Of the total [U-14C]glucose consumed, primary cultures converted approximately 30% to labeled CO2 per hour, whereas the liver cell lines converted 5--10%. Finally, glucose metabolism in primary culture exhibited adaptation as hepatocytes aged in culture, shifting progressively toward the pattern exhibited by the permanent cell lines. This change occurred over a time course similar to that for other kinds of functional change in hepatocytes in primary culture and thus may be relevant to the general problem of phenotypic alteration in liver cell culture.

Characterization of liver cell lines established from WHV-infected woodchucks

1995 ◽  
Vol 121 (S1) ◽  
pp. A61-A61
Author(s):  
D. Mayer ◽  
A. Loktionov ◽  
P. Bannasch
Keyword(s):  
Cell Lines ◽  
Liver Cell

scholarly journals Isolation and Characterization of a Structural Homologue of Human PRK2 from Rat Liver

1997 ◽  
Vol 272 (15) ◽  
pp. 10030-10034 ◽  
Author(s):  
Weiping Yu ◽  
Junjun Liu ◽  
Nicholas A. Morrice ◽  
Richard E. H. Wettenhall
Keyword(s):  
Rat Liver ◽  
Isolation And Characterization ◽  
Structural Homologue
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