An organ culture of postnatal rat liver slices

In Vitro ◽  
1983 ◽  
Vol 19 (11) ◽  
pp. 841-852 ◽  
Author(s):  
Adele Hart ◽  
Frederick J. Mattheyse ◽  
John B. Balinsky
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Liver Slices

Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure–Toxicity Studies

1993 ◽  
pp. 222-230 ◽  
Author(s):  
Klaus Brendel ◽  
Robyn L. Fisher ◽  
Carlos L. Krumdieck ◽  
A. Jay Gandolfi
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Toxicity Studies ◽  
Liver Slices

Maintenance of adult rat liver slices in dynamic organ culture

1986 ◽  
Vol 22 (12) ◽  
pp. 706-712 ◽  
Author(s):  
P. F. Smith ◽  
G. Krack ◽  
R. L. McKee ◽  
D. G. Johnson ◽  
A. J. Gandolfi ◽  
...  
Keyword(s):  
Organ Culture ◽  
Rat Liver ◽  
Adult Rat ◽  
Liver Slices

Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies

1990 ◽  
Vol 9 (6) ◽  
pp. 621-627 ◽  
Author(s):  
Klaus Brendel ◽  
Robyn L. Fisher ◽  
Carlos L. Krumdieck ◽  
A. Jay Gandolfi
Keyword(s):  
Fatty Acids ◽  
Organ Culture ◽  
Chain Length ◽  
Rat Liver ◽  
Sprague Dawley ◽  
Toxicity Studies ◽  
Liver Slices ◽  
Sprague Dawley Rat ◽  
Branched Chain

We describe a novel in vitro mammalian liver system, which simplifies both metabolism and hepatotoxicity studies in a multitude of species. This system is based on mechanical cutting of slices with high precision and culture of such slices under dynamic organ culture conditions. This system permits study of intoxication or metabolism for many hours to days and is suitable for integrative biochemical assays as well as histological and morphological evaluation. We report the toxicity exhibited by the isomeric dichlorobenzenes, which in Sprague-Dawley rat liver slices is M>O>P. We also compared a series of bromobenzene analogs, which in Sprague-Dawley rat liver slices are toxic in the following sequence DBB > BB > BBN > BA > BBT > BT. The toxicity of medium chain length branched-chain fatty acids related to valproic acid also was evaluated in this system. Results show that toxicity increased with chain length in α-methyl fatty acids and depended on the location of the carboxyl group in branched-chain octanoic acids. These examples serve to illustrate the utility of precision-cut rat liver slices in dynamic organ culture for structure—toxicity studies.

OXYDATION DES ANABOLIKUMS 1-METHYL-ANDROST-1-EN-17β-OL-3-ON MIT WASSERSTOFFTRANSFER AUF ÖSTRON

1971 ◽  
Vol 67 (3) ◽  
pp. 517-530 ◽  
Author(s):  
Martin Wenzel
Keyword(s):  
Rat Liver ◽  
Anabolic Steroid ◽  
Female Rats ◽  
Male Rats ◽  
Female Rat ◽  
Liver Slices ◽  
Androgen Effects ◽  
Biochemical Difference

ABSTRACT With the aid of metenolon-17α-T a tritium-transfer to oestrone in rat liver slices was demonstrated. This tritium-transfer from metenolon17α-T to oestrone yielding tritium-labelled oestradiol had a higher efficiency in male than in female rat liver. Correspondingly in the presence of metenolon the relation of oestrone to oestradiol is changed more in male than in female rat liver. Looking for biochemical differences between the anabolic steroid metenolon and testosterone the oxydation at C17 was measured in different organs of the rat using 17α-T-labelled steroids. The highest oxydation rate was found for both steroids in the liver. In the sexual organs of male rats the oxydation rate of testosterone was 50–10 times higher than that of the anabolic steroid. This difference was less in sexual organs of female rats. This result of a greater biochemical difference between both steroids in males than in females leads to the question, whether the dissociation between the anabolic and the androgen effects is higher in males than in females.

scholarly journals THE ESTIMATION OF FATTY ACID SYNTHESIS IN RAT LIVER SLICES

1953 ◽  
Vol 205 (1) ◽  
pp. 401-408
Author(s):  
Grace Medes ◽  
Morris A. Spirtes ◽  
Sidney Weinhouse
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Liver Slices

Influence of Slice Thickness and Culture Conditions on the Metabolism of 7-Ethoxycoumarin in Precision-cut Rat Liver Slices

1998 ◽  
Vol 26 (4) ◽  
pp. 541-548
Author(s):  
Roger J. Price ◽  
Anthony B. Renwick ◽  
Paula T. Barton ◽  
J. Brian Houston ◽  
Brian G. Lake
Keyword(s):  
Gas Phase ◽  
Rat Liver ◽  
Xenobiotic Metabolism ◽  
Culture Conditions ◽  
Slice Thickness ◽  
Dependent Manner ◽  
Sprague Dawley ◽  
Liver Slices ◽  
Sprague Dawley Rats ◽  
Rat Livers

This study investigated the effects of some experimental variables on the rate of xenobiotic metabolism in precision-cut rat liver slices. Liver slices of 123 ± 8μm (mean ± SEM of six slices), 165 ± 3μm, 238 ± 6μm and 515 ± 14μm thickness were prepared from male Sprague-Dawley rats, and incubated in RPMI 1640 medium in an atmosphere of 95% O2/5% CO2 by using a dynamic organ culture system. Liver slices of all thicknesses metabolised 10μM 7-ethoxycoumarin to total (free and conjugated) 7-hydroxycoumarin in a time-dependent manner. The rate of 7-ethoxycoumarin metabolism was greatest in 165μm thick slices and slowest in 515μm thick slices, being 2.74 ± 0.19pmol/minute/mg slice protein and 0.69 ± 0.07pmol/minute/mg slice protein, respectively. No marked effects on the rate of 7-ethoxycoumarin metabolism in liver slices were observed either by changing the medium to Earle's balanced salt solution (EBSS) or by changing the gas phase to 95% air/5% CO2. Moreover, the perfusion of rat livers with EBSS at 2–4°C, prior to preparation of tissue cores, did not enhance 7-ethoxycoumarin metabolism in rat liver slices. In this study, the optimal slice thickness was 175μm, with higher rates of 7-ethoxycoumarin metabolism being observed than with 250μm thick slices, which are often used for studies of xenobiotic metabolism. Variable results were obtained with slices of around 100–120μm thickness, which may be attributable to the ratio between intact hepatocytes and cells damaged by the slicing procedure in these very thin slices.

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