Some distinctive characteristics of high density perfusion cultures of diverse cell types

In Vitro ◽  
1970 ◽  
Vol 6 (1) ◽  
pp. 75-88 ◽  
Author(s):  
Paul F. Kruse ◽  
Lynn N. Keen ◽  
Wilbur L. Whittle
Keyword(s):  
Cell Types ◽  
High Density ◽  
Perfusion Cultures

scholarly journals RhoA localization with caveolin-1 regulates vascular contractions to serotonin

2012 ◽  
Vol 303 (9) ◽  
pp. R959-R967 ◽  
Author(s):  
Daniel W. Nuno ◽  
Sarah K. England ◽  
Kathryn G. Lamping
Keyword(s):  
Lipid Rafts ◽  
Rho Kinase ◽  
Initial Response ◽  
Cell Types ◽  
Smooth Muscle Contraction ◽  
High Density ◽  
Low Density ◽  
Density Fractions ◽  
Caveolin 1 ◽  
Multiple Cell

Vascular smooth muscle contraction occurs following an initial response to an increase in intracellular calcium concentration and a sustained response following increases in the sensitivity of contractile proteins to calcium (calcium sensitization). This latter process is regulated by the rhoA/rho kinase pathway and activated by serotonin. In multiple cell types, signaling molecules compartmentalize within caveolae to regulate their activation. We hypothesized that serotonin differentially compartmentalizes rhoA within caveolar versus noncaveolar lipid rafts to regulate sustained vascular contractions. To test this hypothesis, we measured aortic contractions in response to serotonin in wild-type (WT) and cav-1-deficient mice (cav-1 KO). RhoA-dependent contractions in response to serotonin were markedly augmented in arteries from cav-1 KO mice despite a modest reduction in rhoA expression compared with WT. We found that under basal conditions, rhoA in WT arteries was primarily localized within high-density sucrose gradient fractions but temporally shifted to low-density fractions in response to serotonin. In contrast, rhoA in cav-1 KO arteries was primarily in low-density fractions and shifted to high-density fractions in a similar timeframe as that seen in WT mice. We conclude that localization of rhoA to caveolar versus noncaveolar lipid rafts differentially regulates its activation and contractions to rhoA-dependent agonists with greater activation associated with its localization to noncaveolar rafts. Disruption of rhoA localization within caveolae may contribute to increased activation and enhanced vascular contractions in cardiovascular disease.

The Use of Peptones as Medium Additives for High-Density Perfusion Cultures of Animal Cells

2008 ◽  
pp. 195-197
Author(s):  
R. Heidemann ◽  
C. Zhang ◽  
H. Qi ◽  
J. Rule ◽  
C. Rozales ◽  
...  
Keyword(s):  
High Density ◽  
Animal Cells ◽  
Perfusion Cultures

OUR AND CER ESTIMATION IN HIGH DENSITY MAMMALIAN CELL PERFUSION CULTURES

2007 ◽  
Vol 40 (4) ◽  
pp. 91-96 ◽  
Author(s):  
C. Goudar ◽  
K. Joeris ◽  
C. Cruz ◽  
C. Zhang ◽  
K. Konstantinov
Keyword(s):  
Mammalian Cell ◽  
High Density ◽  
Perfusion Cultures

The Art & Science of Micro-Sparging in High-Density Perfusion Cultures of Animal Cells

2001 ◽  
pp. 412-415 ◽  
Author(s):  
Hanshi Qi ◽  
Goran Jovanoic ◽  
James Michaels ◽  
Konstantin Konstantinov
Keyword(s):  
High Density ◽  
Animal Cells ◽  
Perfusion Cultures

Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures

2010 ◽  
pp. 837-841 ◽  
Author(s):  
C. Cruz ◽  
K. Joeris ◽  
C. Goudar ◽  
C. Zhang ◽  
K. Konstantinov
Keyword(s):  
Oxygen Uptake ◽  
Mammalian Cell ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
High Density ◽  
Perfusion Cultures

scholarly journals A SMALL PARTICULATE COMPONENT OF THE CYTOPLASM

1955 ◽  
Vol 1 (1) ◽  
pp. 59-68 ◽  
Author(s):  
George E. Palade
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Types ◽  
High Density ◽  
Ground Substance ◽  
Degree Of Differentiation ◽  
High Degree

A particulate component of small dimensions (100 to 150 A) and high density is described in the ground substance of the cytoplasm of mammalian and avian cells. In many cell types that seem to have in common a high degree of differentiation, the new component is preferentially associated with the membrane of the endoplasmic reticulum; whereas in other cell types, characterized by rapid proliferation, it occurs more or less freely distributed in the ground substance of the cytoplasm. In the Discussion an attempt is made to integrate the observations presented in this paper with the already available cytological, histochemical, and cytochemical information.

Software sensors for the monitoring of high-density hybridoma perfusion cultures

1994 ◽  
pp. 379-382
Author(s):  
F. PELLETIER ◽  
C. FONTEIX ◽  
A. LOURENÇO da SILVA ◽  
J.L. GOERGEN ◽  
A. MARC ◽  
...  
Keyword(s):  
High Density ◽  
Software Sensors ◽  
Perfusion Cultures

scholarly journals Autocrine signals enable chondrocytes to survive in culture.

1994 ◽  
Vol 126 (4) ◽  
pp. 1069-1077 ◽  
Author(s):  
Y Ishizaki ◽  
J F Burne ◽  
M C Raff
Keyword(s):  
Epithelial Cells ◽  
Cell Density ◽  
Mammalian Cells ◽  
Cell Types ◽  
High Density ◽  
Lens Epithelial Cells ◽  
Low Density ◽  
Cell Type ◽  
Adult Rats ◽  
And Cartilage

We recently proposed that most mammalian cells other than blastomeres may be programmed to kill themselves unless continuously signaled by other cells not to. Many observations indicate that some mammalian cells are programmed in this way, but is it the case for most mammalian cells? As it is impractical to test all of the hundreds of types of mammalian cells, we have focused on two tissues--lens and cartilage--which each contain only a single cell type: if there are cells that do not require signals from other cells to avoid programmed cell death (PCD), lens epithelial cells and cartilage cells (chondrocytes) might be expected to be among them. We have previously shown that rat lens epithelial cells can survive in serum-free culture without signals from other cell types but seem to require signals from other lens epithelial cells to survive: without such signals they undergo PCD. We show here that the same is true for rat (and chick) chondrocytes. They can survive for weeks in culture at high cell density in the absence of other cell types, serum, or exogenous proteins or signaling molecules, but they die with the morphological features of apoptosis in these conditions at low cell density. Medium from high density cultures, FCS, or a combination of known growth factors, all support prolonged chondrocyte survival in low density cultures, as long as antioxidants are also present. Moreover, medium from high density chondrocyte cultures promotes the survival of lens epithelial cells in low density cultures and vice versa. Chondrocytes isolated from adult rats behave similarly to those isolated from developing rats. These findings support the hypothesis that most mammalian cells require signals from other cells to avoid PCD, although the signals can sometimes be provided by cells of the same type, at least in tissues that contain only one cell type.

scholarly journals Filamin A–β1 Integrin Complex Tunes Epithelial Cell Response to Matrix Tension

2009 ◽  
Vol 20 (14) ◽  
pp. 3224-3238 ◽  
Author(s):  
Scott Gehler ◽  
Massimiliano Baldassarre ◽  
Yatish Lad ◽  
Jennifer L. Leight ◽  
Michele A. Wozniak ◽  
...  
Keyword(s):  
Collagen Matrix ◽  
Cell Types ◽  
Branching Morphogenesis ◽  
High Density ◽  
Actin Binding ◽  
Β1 Integrin ◽  
Filamin A ◽  
Collagen Gels ◽  
Collagen Remodeling ◽  
The Matrix

The physical properties of the extracellular matrix (ECM) regulate the behavior of several cell types; yet, mechanisms by which cells recognize and respond to changes in these properties are not clear. For example, breast epithelial cells undergo ductal morphogenesis only when cultured in a compliant collagen matrix, but not when the tension of the matrix is increased by loading collagen gels or by increasing collagen density. We report that the actin-binding protein filamin A (FLNa) is necessary for cells to contract collagen gels, and pull on collagen fibrils, which leads to collagen remodeling and morphogenesis in compliant, low-density gels. In stiffer, high-density gels, cells are not able to contract and remodel the matrix, and morphogenesis does not occur. However, increased FLNa-β1 integrin interactions rescue gel contraction and remodeling in high-density gels, resulting in branching morphogenesis. These results suggest morphogenesis can be “tuned” by the balance between cell-generated contractility and opposing matrix stiffness. Our findings support a role for FLNa-β1 integrin as a mechanosensitive complex that bidirectionally senses the tension of the matrix and, in turn, regulates cellular contractility and response to this matrix tension.

scholarly journals Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Kelly A. Landers ◽  
Michael C. d’Emden ◽  
Kerry Richard
Keyword(s):  
High Density Lipoprotein ◽  
Scavenger Receptor ◽  
Density Lipoprotein ◽  
Cell Types ◽  
Normal Function ◽  
High Density ◽  
Scavenger Receptor Class ◽  
Class B ◽  
Density Lipoprotein Receptor ◽  
And Function

Thyroid hormone (thyroxine, T4) is essential for the normal function of all cell types and is carried in serum bound to several proteins including transthyretin. Recently, evidence has emerged of alternate pathways for hormone entry into cells that are dependent on hormone binding proteins. Transthyretin and transthyretin bound T4 are endocytosed by placental trophoblasts through the high-density lipoprotein receptor, Scavenger Receptor Class B Type 1 (SR-B1). High density lipoprotein (HDL) affects the expression and function of SR-B1 in trophoblast cells. SR-B1 is also expressed in hepatocytes and we sought to determine if hepatocyte SR-B1 was involved in transthyretin or transthyretin-T4 uptake and whether uptake was affected by HDL. Transthyretin and transthyretin-T4 uptake by hepatocytes is not dependent on SR-B1. HDL treatment reduced SR-B1 expression. However, pretreatment of hepatocytes with HDL increased uptake of transthyretin-T4. Knockdown of SR-B1 expression using siRNA also increased transthyretin-T4 uptake. Coaddition of HDL to transthyretin uptake experiments blocked both transthyretin and transthyretin-T4 uptake. Hepatocyte uptake of transthyretin-T4 uptake is influenced by, but is not dependent on, SR-B1 expression. HDL also decreases transthyretin-T4 uptake and therefore diet or drugs may interfere with this process. This suggests that multiple lipoprotein receptors may be involved in the regulation of uptake of transthyretin-T4 in a cell-type specific manner. Further study is required to understand this important process.

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