Long-term storage of tissue samples for cell culture

In Vitro ◽  
1978 ◽  
Vol 14 (5) ◽  
pp. 476-478 ◽  
Author(s):  
Harold A. Taylor ◽  
Susan E. Riley ◽  
Stephen E. Parks ◽  
Roger E. Stevenson
Keyword(s):  
Cell Culture ◽  
Tissue Samples ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Fishes collected during the 2017 MarineGEO assessment of Kāne‘ohe Bay, O‘ahu, Hawai‘i

2020 ◽  
Vol 100 (4) ◽  
pp. 607-637
Author(s):  
Lynne R. Parenti ◽  
Diane E. Pitassy ◽  
Zeehan Jaafar ◽  
Kirill Vinnikov ◽  
Niamh E. Redmond ◽  
...  
Keyword(s):  
Smithsonian Institution ◽  
Genetic Distances ◽  
Taxonomic Identification ◽  
Tissue Samples ◽  
Long Term Storage ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
Term Storage ◽  
I Gene

AbstractWe report the results of a survey of the fishes of Kāne‘ohe Bay, O‘ahu, conducted in 2017 as part of the Smithsonian Institution MarineGEO Hawaii bioassessment. We recorded 109 species in 43 families. The most speciose families were Acanthuridae (11 species), Gobiidae (11 species), Pomacentridae (10) and Chaetodontidae (9 species). Nine of the species that we collected are known or suspected to be introduced to the Hawaiian Islands. Specimens were identified, measured and photographed. All specimen vouchers were fixed in formalin and ultimately transferred to 75% ethanol for long-term storage. For nearly all species, we took multiple tissue samples from specimen vouchers prior to formalin-fixation; we preserved tissues in 95% ethanol and then stored them at −80°C. The 5′-end of the mitochondrial cytochrome oxidase I gene (mtCOI) was sequenced for 94 species to confirm their taxonomic identification. Using these barcode sequences, we also measured genetic distances between collected individuals and their conspecifics from other localities outside Hawaii to verify the hypothesis that Hawaiian populations of species broadly distributed throughout the Indo-Pacific may be genetically distinct. We present select case studies to demonstrate the potential for undiscovered endemism in the Hawaiian fish biota.

scholarly journals Impaired performance of SARS-CoV-2 antigen-detecting rapid tests at elevated temperatures

2021 ◽  
Author(s):  
Verena Haage ◽  
Edmilson Ferreira de Oliveira-Filho ◽  
Andres Moreira-Soto ◽  
Arne Kühne ◽  
Carlo Fischer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Elevated Temperatures ◽  
False Negative ◽  
Storage Conditions ◽  
World Health ◽  
Cell Culture Supernatant ◽  
Analytical Sensitivity ◽  
Long Term Storage ◽  
Term Storage

AbstractRapid antigen-detecting tests (Ag-RDTs) can complement molecular diagnostics for COVID-19. The recommended temperature for storage of SARS-CoV-2 Ag-RDTs ranges between 5-30°C. In many countries that would benefit from SARS-CoV-2 Ag-RDTs, mean temperatures exceed 30°C. We assessed analytical sensitivity and specificity of eleven commercially available SARS-CoV-2 Ag-RDTs using different storage and operational temperatures, including (i) long-term storage and testing at recommended conditions, (ii) recommended storage conditions followed by 10 minutes exposure to 37°C and testing at 37°C and (iii) 3 weeks storage followed by testing at 37°C. The limits of detection of SARS-CoV-2 Ag-RDTs under recommended conditions ranged from 8.2×105-7.9×107 genome copies/ml of infectious SARS-CoV-2 cell culture supernatant. Despite long-term storage at recommended conditions, 10 minutes pre-incubation of Ag-RDTs and testing at 37°C resulted in about ten-fold reduced sensitivity for 46% of SARS-CoV-2 Ag-RDTs, including both Ag-RDTs currently listed for emergency use by the World Health Organization. After 3 weeks of storage at 37°C, 73% of SARS-CoV-2 Ag-RDTs exhibited about ten-fold reduced sensitivity. Specificity of SARS-CoV-2 Ag-RDTs using cell culture-derived human coronaviruses HCoV-229E and HCoV-OC43 was not affected by storage and testing at 37°C. In summary, short- and long-term exposure to elevated temperatures likely impairs sensitivity of several SARS-CoV-2 Ag-RDTs that may translate to false-negative test results at clinically relevant virus concentrations compatible with inter-individual transmission. Ensuring appropriate transport and storage conditions, and development of tests that are more robust across temperature fluctuations will be important for accurate use of SARS-CoV-2 Ag-RDTs in tropical settings.

scholarly journals RNA-Integrity and 8-Isoprostane Levels Are Stable in Prostate Tissue Samples Upon Long-Term Storage at −80°C

2020 ◽  
Author(s):  
Sandra Amalie Dybos ◽  
Åge Winje Brustad ◽  
Toril Rolfseng ◽  
Solveig Kvam ◽  
Oddrun Elise Olsen ◽  
...  
Keyword(s):  
Prostate Tissue ◽  
Tissue Samples ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Term Storage

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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