Response of mammalian cells to controlled growth rates in steady-state continuous culture

In Vitro ◽  
1974 ◽  
Vol 10 (5-6) ◽  
pp. 295-305 ◽  
Author(s):  
R. Sinclair
Keyword(s):  
Steady State ◽  
Continuous Culture ◽  
Growth Rates ◽  
Mammalian Cells ◽  
Controlled Growth

"Steady-state" competence for bacterial transformation

1968 ◽  
Vol 14 (3) ◽  
pp. 290-292
Author(s):  
Herbert H. Eichhorn ◽  
Shigeyo Arikawa ◽  
Stephen Zamenhof
Keyword(s):  
Bacillus Subtilis ◽  
Steady State ◽  
Continuous Culture ◽  
Generation Time ◽  
Bacterial Transformation ◽  
Short Period ◽  
Minimal Media ◽  
Harmful Effects ◽  
Specific Phase

The cells of Bacillus subtilis, grown in minimal media, are known to become competent for transformation for a short period at a specific phase of ceil growth. In the present work the cells (strain 168 ind−) were grown in continuous culture (chemostat; glucose limiting, generation time 4 h, 37 °C). Aliquots were removed at 20- to 24-h intervals and immediately tested for competence. The viability (41 h) was 97%. The initial very low competence increased up to 200-fold within the 24 h and remained at this high, slowly decreasing level for at least 168 h. It is concluded that a long-lasting competence may develop and persist in the cells in continuous culture ("steady-state"), without demonstrable harmful effects to the population.

Metabolic flux analysis of hybridoma continuous culture steady state multiplicity

1999 ◽  
Vol 63 (6) ◽  
pp. 675-683 ◽  
Author(s):  
Brian D. Follstad ◽  
R. Robert Balcarcel ◽  
Gregory Stephanopoulos ◽  
Daniel I. C. Wang
Keyword(s):  
Steady State ◽  
Continuous Culture ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
State Multiplicity

scholarly journals Behavior of chlorella pyrenoidosa in steady state continuous culture

1970 ◽  
Author(s):  
Joseph N. Dabes ◽  
Charles R. Wilke ◽  
Kenneth H. Sauer
Keyword(s):  
Steady State ◽  
Continuous Culture ◽  
Chlorella Pyrenoidosa

scholarly journals Estimates of the steady state growth rates for some European countries

2012 ◽  
Vol 29 (4) ◽  
pp. 1119-1125 ◽  
Author(s):  
Paolo Casadio ◽  
Antonio Paradiso ◽  
B. Bhaskara Rao
Keyword(s):  
Steady State ◽  
Growth Rates ◽  
European Countries ◽  
Steady State Growth ◽  
State Growth

scholarly journals Successive Paradigm Shifts in the Bacterial Cell Cycle and Related Subjects

Life ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 27
Author(s):  
Vic Norris
Keyword(s):  
Cell Cycle ◽  
Steady State ◽  
Growth Rates ◽  
Bacterial Cell ◽  
Paradigm Shift ◽  
Eukaryotic Cell ◽  
Origins Of Life ◽  
Paradigm Shifts ◽  
Bacterial Physiology ◽  
Bacterial Cell Cycle

A paradigm shift in one field can trigger paradigm shifts in other fields. This is illustrated by the paradigm shifts that have occurred in bacterial physiology following the discoveries that bacteria are not unstructured, that the bacterial cell cycle is not controlled by the dynamics of peptidoglycan, and that the growth rates of bacteria in the same steady-state population are not at all the same. These paradigm shifts are having an effect on longstanding hypotheses about the regulation of the bacterial cell cycle, which appear increasingly to be inadequate. I argue that, just as one earthquake can trigger others, an imminent paradigm shift in the regulation of the bacterial cell cycle will have repercussions or “paradigm quakes” on hypotheses about the origins of life and about the regulation of the eukaryotic cell cycle.

Kinetic analysis of batch and continuous culture of Streptococcus cremoris HP

1978 ◽  
Vol 24 (4) ◽  
pp. 372-380 ◽  
Author(s):  
P. L. Rogers ◽  
L. Bramall ◽  
I. J. McDonald
Keyword(s):  
Lactic Acid ◽  
Steady State ◽  
Kinetic Analysis ◽  
Continuous Culture ◽  
Batch Culture ◽  
Lactic Acid Production ◽  
Streptococcus Cremoris ◽  
Lactose Utilization ◽  
State Growth ◽  
Batch Data

The growth of Streptococcus cremoris on a semidefined medium was studied at initial lactose concentrations of 0.2–5.0% in batch culture, and in lactose-limited chemostat cultures at 0.5% lactose. Kinetic analysis of the batch data, using statistical techniques, indicated the importance of lactose limitation and lactic acid inhibition of the growth of S. cremoris. A model for the biomass production, lactose utilization, and lactic acid production in batch culture was proposed. In continuous culture, it was found that steady state populations were maintained at higher dilution rates (D = 0.6–0.7 h−1) than the maximum predicted by batch culture (0.56 h−1). No evidence for a selection of fast-growing mutants was obtained. Copious growth adhering to the walls of the fermentor (i.e. wall growth) occurred very rapidly at higher dilution rates and this undoubtedly affected steady-state growth and wash-out and, as a consequence, the apparent maximum dilution rate.

scholarly journals Nonlinear interactions among standing surface and internal gravity waves

1974 ◽  
Vol 63 (4) ◽  
pp. 801-825 ◽  
Author(s):  
Terrence M. Joyce
Keyword(s):  
Energy Transfer ◽  
Steady State ◽  
Internal Wave ◽  
Surface Waves ◽  
Viscous Dissipation ◽  
Growth Rates ◽  
Internal Gravity Wave ◽  
Internal Gravity Waves ◽  
Resonant Interaction ◽  
Initial Growth

A laboratory study has been undertaken to measure the energy transfer from two surface waves to one internal gravity wave in a nonlinear, resonant interaction. The interacting waves form triads for which \[ \sigma_{1s} - \sigma_{2s} \pm\sigma_1 = 0\quad {\rm and}\quad \kappa_{1s} - \kappa_2s} \pm \kappa_I = 0; \] σj and κj being the frequency and wavenumber of the jth wave. Unlike previously published results involving single triplets of interacting waves, all waves here considered are standing waves. For both a diffuse, two-layer density field and a linearly increasing density with depth, the growth to steady state of a resonant internal wave is observed while two deep water surface eigen-modes are simultaneously forced by a paddle. Internal-wave amplitudes, phases and initial growth rates are compared with theoretical results derived assuming an arbitrary Boussinesq stratification, viscous dissipation and slight detuning of the internal wave. Inclusion of viscous dissipation and slight detuning permit predictions of steady-state amplitudes and phases as well as initial growth rates. Satisfactory agreement is found between predicted and measured amplitudes and phases. Results also suggest that the internal wave in a resonant triad can act as a catalyst, permitting appreciable energy transfer among surface waves.

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