Induction of placental alkaline phosphatase in choriocarcinoma cells by 5-bromo-2′-deoxyuridine

In Vitro ◽  
1977 ◽  
Vol 13 (7) ◽  
pp. 450-460 ◽  
Author(s):  
Janice Y. Chou ◽  
J. C. Robinson
Keyword(s):  
Alkaline Phosphatase ◽  
Placental Alkaline Phosphatase ◽  
Choriocarcinoma Cells

scholarly journals Expression of different-sized placental alkaline phosphatase mRNAs in placenta and choriocarcinoma cells.

1986 ◽  
Vol 83 (11) ◽  
pp. 3781-3785 ◽  
Author(s):  
C. E. Ovitt ◽  
A. W. Strauss ◽  
D. H. Alpers ◽  
J. Y. Chou ◽  
I. Boime
Keyword(s):  
Alkaline Phosphatase ◽  
Placental Alkaline Phosphatase ◽  
Choriocarcinoma Cells

scholarly journals The effect of bromodeoxyuridine and dibutyryl cyclic AMP on the induction of placental alkaline phosphate in human choriocarcinoma cells

1981 ◽  
Vol 198 (1) ◽  
pp. 29-35 ◽  
Author(s):  
T A Hamilton ◽  
H H Sussman
Keyword(s):  
Alkaline Phosphatase ◽  
Cyclic Amp ◽  
Polyacrylamide Gel Electrophoresis ◽  
Specific Protein ◽  
Placental Alkaline Phosphatase ◽  
Dibutyryl Cyclic Amp ◽  
Specific Enzyme ◽  
Alkaline Phosphate ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

The effect of 5-bromo-2′-deoxyuridine (BrdUrd) and dibutyryl cyclic AMP (Bt2cAMP) on the expression of the placental isoenzyme of human alkaline phosphatase was examined in BeWo choriocarcinoma cells. By using a combination of specific immunoprecipitation and polyacrylamide-gel electrophoresis of cells labelled either metabolically with [35S]methionine or cell-surface-labelled with 125I, both BrdUrd (5 micrograms/ml) and 1 mM-Bt2cAMP were shown to result in the enhanced accumulation of a specific protein. This protein has immunochemical identity and co-electrophoreses with placental alkaline phosphatase in two-dimensional gels. These results clearly demonstrate that the induction of placental alkaline phosphatase activity in choriocarcinoma cells treated with these agents is a consequence of the accumulation of specific enzyme protein rather than of altered catalytic activity.

scholarly journals Expression of placental alkaline phosphatase does not correlate with IgG binding, internalization and transcytosis

1997 ◽  
Vol 327 (2) ◽  
pp. 585-592 ◽  
Author(s):  
Isabella STEFANER ◽  
Anca STEFANESCU ◽  
Walter HUNZIKER ◽  
Renate FUCHS
Keyword(s):  
Alkaline Phosphatase ◽  
Mdck Cells ◽  
Maternal Blood ◽  
Apical Surface ◽  
Placental Alkaline Phosphatase ◽  
Intact Cells ◽  
Blood Ph ◽  
Igg Binding ◽  
Choriocarcinoma Cells ◽  
Darby Canine Kidney

The human homologue of FcRn, an IgG Fc receptor expressed in rat villous syncytiotrophoblasts, might be involved in IgG transfer from the maternal to the fetal circulation. However, because the receptor does not bind IgG at the physiological pH of the maternal blood (pH 7.4), FcRn is probably not involved in the initial uptake of IgG. A role in IgG internalization has been suggested for placental alkaline phosphatase (PLAP), which is highly expressed on the apical surface of syncytiotrophoblasts. To determine whether PLAP does indeed have a role in IgG uptake, we analysed the ability of PLAP to bind, internalize and transcytose IgG in BeWo choriocarcinoma cells endogenously expressing the protein, or in Madin-Darby canine kidney (MDCK) cells transfected with the PLAP cDNA. Although PLAP expression in MDCK cells resulted in increased IgG binding to intact cells, binding was not correlated with the level of PLAP expressed in the different cell lines. Furthermore our findings do not support a role for PLAP in IgG endocytosis or transcytosis.

scholarly journals GCT-06. DIAGNOSIS OF A RARE CASE OF RECURRENT GERM CELL TUMOR BY CSF PLACENTAL ALKALINE PHOSPHATASE PRESENTING WITH DIFFUSE INTRAAXIAL ABNORMALITY IN THE LOWER BRAINSTEM

2020 ◽  
Vol 22 (Supplement_3) ◽  
pp. iii329-iii329
Author(s):  
Hiroki Yamada ◽  
Tomohiro Abiko ◽  
Hirokazu Fujiwara ◽  
Kazunari Yoshida ◽  
Hikaru Sasaki
Keyword(s):  
Alkaline Phosphatase ◽  
Germ Cell ◽  
Germ Cell Tumor ◽  
Rare Case ◽  
Cervical Spinal Cord ◽  
Germ Cell Tumors ◽  
Cell Tumor ◽  
Placental Alkaline Phosphatase ◽  
Steroid Pulse ◽  
Platinum Based Chemotherapy

Abstract INTRODUCTION Germ cell tumors in the central nervous system (CNS) typically arise either at suprasellar and/or pineal region, and occasionally at basal ganglia. We report a case of diagnostically challenging, recurrent germ cell tumor presented with diffuse intraaxial abnormality in and across the lower brainstem, which was diagnosed by the elevated placental alkaline phosphatase (PLAP) level in cerebrospinal fluid (CSF). CASE DESCRIPTION: A 28-year-old man had been treated by chemoradiotherapy at the previous hospital for bifocal suprasellar and pineal lesions with the provisional diagnosis of germinoma without histological confirmation. Three years later, he presented with progressive weakness of bilateral extremities for weeks. Magnetic resonance imaging showed a diffuse, bilaterally symmetric high intensity lesion on T2-weighted image with slight contrast enhancement across the ventral side of the medulla oblongata to the upper cervical spinal cord. Serum and CSF hCG, hCG-β, and AFP were all negative. Since the image findings were atypical for recurrent germ cell tumor, some kind of myelitis was initially suspected. Therefore, steroid pulse therapy was administered. However, the patient’s symptom was still gradually progressing. Then, the CSF PLAP turned out to be positive, indicating the recurrence of germinoma. Accordingly, platinum-based chemotherapy was administered, and the imaging findings, patient’s symptoms, and CSF PLAP began to improve. The patient is to be treated with radiotherapy following chemotherapy. CONCLUSION We report a rare case of CNS germ cell tumor that presented with diffuse intraaxial lesion in the lower brainstem in which examination of CSF PLAP was extremely useful.

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