Suspension culture of drosophila cells employing a gyratory shaker

In Vitro ◽  
1977 ◽  
Vol 13 (4) ◽  
pp. 245-251 ◽  
Author(s):  
T. Miyake ◽  
K. Saigo ◽  
T. Marunouchi ◽  
T. Shiba
Keyword(s):  
Suspension Culture ◽  
Gyratory Shaker ◽  
Drosophila Cells

Immobilization of Rhodococcus wratislaviensis Strain KT112-7 Сells in Order to Increase Efficiency of Biodegradation of Modified Polychlorinated Biphenyls

2019 ◽  
pp. 58-70
Author(s):  
M.G. Pervova ◽  
T.I. Gorbunova ◽  
V.A. Demakov
Keyword(s):  
Activated Carbon ◽  
Carbon Fiber ◽  
Polychlorinated Biphenyls ◽  
Suspension Culture ◽  
Chemically Modified ◽  
Commercial Mixture ◽  
Immobilized Culture ◽  
Rhodococcus Wratislaviensis ◽  
Academy Of Sciences ◽  
Integrated Program

The degradation of a mixture of hydroxy- and methoxy-PCB obtained as a result of a chemical modification of a commercial mixture of PCB «Sovol» by the Rhodococcus wratislaviensis strain KT112-7 in plankton culture or immobilized on carbon carriers has been investigated. It was established that the KT112-7 strain in plankton culture degraded 73.2% of a mixture of modified PCBs for 96 h; the strain immobilized on BAU-A activated carbon and on Carbopon-B-active carbon fiber provided the 59.5% and 95.3% degradation for the same time, respectively (with the starting concentration of PBS of 0.5 g/L). The application of the R. wratislaviensis KT112-7 strain immobilized on the BAU-A carrier decreased the number of the PCB derivatives by 1.5 times at the end of the experiment; the corresponding result for the Carbopon-B-active-immobilized culture was 3 times; the set of PCBs after the treatment with the suspension culture remained unchanged. In practice, the immobilization of R. wratislaviensis КТ112-7 strain on carbon carriers can be used in the development of the techniques for the degradation of chemically modified PCB mixtures. polychlorinated biphenyls, immobilization, destruction, utilization, PCB, Rhodococcus. Funding-This work was supported by the Integrated Program of the Ural Branch of the Russian Academy of Sciences No. 18-3-8-19. The work was performed using the equipment of the Center for collective use «Spectroscopy and analysis of organic compounds».

Anthocyanin accumulation and PAL activity in a suspension culture of Daucus carota L.

Planta ◽  
1980 ◽  
Vol 149 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Wolfgang No� ◽  
Christian Langebartels ◽  
Hanns Ulrich Seitz
Keyword(s):  
Suspension Culture ◽  
Daucus Carota ◽  
Anthocyanin Accumulation ◽  
Daucus Carota L ◽  
Pal Activity

Callus induction, suspension culture and protoplast isolation in Camellia oleifera

2021 ◽  
Vol 286 ◽  
pp. 110193
Author(s):  
Su-Fang Li ◽  
Tian-Wen Ye ◽  
Xin Xu ◽  
De-Yi Yuan ◽  
Shi-Xin Xiao
Keyword(s):  
Suspension Culture ◽  
Callus Induction ◽  
Protoplast Isolation ◽  
Camellia Oleifera

scholarly journals Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C

2021 ◽  
Vol 11 (4) ◽  
pp. 1941
Author(s):  
Seungmin Yeom ◽  
Myung Chul Lee ◽  
Shambhavi Pandey ◽  
Jaewoon Lim ◽  
Sangbae Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Stem Cell ◽  
Vitamin C ◽  
Suspension Culture ◽  
Small Molecules ◽  
Tumor Development ◽  
Embryonic Stem ◽  
Short Term ◽  
External Stimuli

Induced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor development. The goal of this research was to develop an alternative method for reprogramming, using small molecules and external stimuli. Two groups were established: short-term shear stress (STSS) under suspension culture and a combination of short-term shear stress and vitamin C (SSVC) under suspension culture. For STSS, the pipetting was carried out for cells twice per day for 2 min for 14 days in the embryonic stem cell (ES) medium. In the case of SSVC, the procedure was the same as for STSS however, its ES medium included 10 µM of vitamin C. After 14 days, all spheroids were picked and checked for pluripotency by ALP (alkaline phosphatase) assay and immunocytochemistry. Both groups partially showed the characteristics of stem cells but data demonstrated that the spheroids under shear stress and vitamin C had improved stem cell-like properties. This research showed the possibility of external stimuli and small molecules to reprogram the somatic cells without the use of transgenes.

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