Determination of L-ascorbic acid levels in culture medium: Concentrations in commercial media and maintenance of levels under conditions of organ culture

In Vitro ◽  
1977 ◽  
Vol 13 (2) ◽  
pp. 91-99 ◽  
Author(s):  
J. Feng ◽  
A. H. Melcher ◽  
D. M. Brunette ◽  
H. K. Moe
Keyword(s):  
Ascorbic Acid ◽  
Organ Culture ◽  
Culture Medium ◽  
Commercial Media

The effects of ascorbic acid deficiency on collagen synthesis by mouse molar tooth germs in organ culture

Development ◽  
1977 ◽  
Vol 37 (1) ◽  
pp. 49-57
Author(s):  
John R. Schiltz ◽  
Joel Rosenbloom ◽  
Gordon E. Levenson
Keyword(s):  
Ascorbic Acid ◽  
Organ Culture ◽  
Culture Medium ◽  
Large Fraction ◽  
Culture Conditions ◽  
Molar Tooth ◽  
Second Molar ◽  
Acid Deficiency ◽  
Tooth Germs

Second molar tooth germs from 2-day-old Swiss-Webster mice, grown in organ culture for 7 days in ascorbic-acid-deficient medium, synthesized about 65 % as much protein (measured by incorporation of [14C]proline during a 24-h pulse) as did ascorbic-acid-supplemented controls. The newly synthesized proteins from ascorbic-acid-deficient cultures contained only about 7% of the hydroxyproline content of the controls. Collagenase digestion of the newly synthesized proteins showed that collagen comprised the same fraction of the total protein synthesized under both culture conditions. This result indicates that the ascorbatedeficient cultures made significant quantities of underhydroxylated collagen. Partial characterization of the collagen alpha chains on carboxymethyl cellulose columns showed an α1/α2 ratio of about 5, suggesting that at least two different species of collagen were synthesized. The α1/α2 ratio of the chains recovered from the ascorbate-deficient cultures was also about 5 but the chains were slightly underhydroxylated and the total amount of these chains which could be identified accounted for only a small fraction of the total collagen which was synthesized. A large fraction of the synthesized collagenous protein was found in the culture medium, mostly in the form of lower molecular weight peptides. It is concluded that most of the collagen which is synthesized by ascorbate-deficient tooth-bud cultures is not utilized by the component tissues, but is probably degraded and released into the medium.

Fluorometric determination of ascorbic acid in the absence of the oxidant in juices of common citrus

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A Copra-Janicijevic ◽  
E Sofic ◽  
L Klepo ◽  
A Topcagic ◽  
I Tahirovic ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorometric Determination

The Effect of Stress and Warfarin on the Adrenal Gland in Relation to Spontaneous Hemorrhage, as Judged by Measurement of Adrenal Ascorbic Acid and Serum Corticosterone

1971 ◽  
Vol 26 (02) ◽  
pp. 275-288 ◽  
Author(s):  
S Chattopadhyay ◽  
D. D Johnson ◽  
G. J Millar ◽  
L. B Jaques
Keyword(s):  
Ascorbic Acid ◽  
Acid Concentration ◽  
Ascorbic Acid Concentration ◽  
Corticosterone Concentration ◽  
Serum Corticosterone ◽  
Spontaneous Hemorrhage ◽  
Combined Action ◽  
Series Of Experiments ◽  
Pituitary Adrenal Axis

SummaryRats were subjected to the following procedures: No treatment, Stressor (10% NaCl i.p.), Warfarin for 7 days, Stressor followed by Warfarin; and groups were sacrificed at intervals for assessment of spontaneous hemorrhage and of adrenal ascorbic acid concentration. There was no hemorrhage in the no treatment and stressor groups; some hemorrhage in the warfarin group; profound hemorrhage with Warfarin + Stressor. The adrenal ascorbic acid concentration was found to be lower, 8 h and again 5 days after stress, and remained lower in the warfarin + stress animals. Warfarin had no effect on adrenal ascorbic acid level.In another series of experiments in which the stress consisted of an electric current to the cage floor for 6 sec over 15 min, rats were sacrificed daily for determination of serum corticosterone concentration and occurrence of spontaneous hemorrhage. There was a statistically significant increase of serum corticosterone concentration with stress, warfarin and combined warfarin and stress treatments (P< 0.001 for all three variables). There was a significant correlation (r = 0.96 and 0.89, P< 0.01) for serum corticosterone concentration with hemorrhage score and incidence of hemorrhage in stressed rats receiving warfarin, but not in those receiving only warfarin. The results indicate an activation, rather than an exhaustion, of the pituitary-adrenal axis during the combined action of anticoagulant and stress, which results in the development of spontaneous hemorrhage.

ADENOHYPOPHYSEAL LH CONTENT IN NORMAL, ANDROGEN-STERILIZED AND PROGESTERONE-PRIMED STERILE FEMALE RATS

1962 ◽  
Vol 39 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Roger A. Gorski ◽  
Charles A. Barraclough
Keyword(s):  
Ascorbic Acid ◽  
Electrical Stimulation ◽  
Quantitative Determination ◽  
Median Eminence ◽  
Hypothalamic Stimulation ◽  
Female Rats ◽  
Rat Pituitary ◽  
Gland Content ◽  
Stimulation Of

ABSTRACT We have previously suggested that the failure of the androgen-sterilized, persistent-oestrous rat to ovulate, following electrical stimulation of the median eminence structures of the hypothalamus, is due to an insufficiency in adenohypophyseal LH concentration. Using the ovarian ascorbic acid technique for quantitative determination of pituitary LH content, the present studies have demonstrated that the sterile rat pituitary gland contains one-third the LH content of the normal prooestrous gland. Furthermore, not only does progesterone priming of this persistent-oestrous rat result in a 75 % increase in LH concentration, but on hypothalamic stimulation sufficient LH is released to induce ovulation. The decrease in LH concentration which accompanies ovulation in the progesterone-primed, sterile rat is approximately 45 % of the total gland content as compared with a 51 % decrease in pituitary content in the normal cyclic rat.

Determination of the Content of Ascorbic Acid and Antioxidant Capacity of Fruit Buchenavia tomentosa Eichler

2019 ◽  
Vol 11 (3) ◽  
pp. 771-784
Author(s):  
Elisa Tatiana S. Damasceno ◽  
Regiamara R. Almeida ◽  
Bruna C. Pires ◽  
Flavia Viana A. Dutra ◽  
Keyller B. Borges ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity

Pentafluorophenyl bonded phase separation: the assay of Australian antioxidant formulation of ascorbic acid, rutin and hesperidin

2017 ◽  
Vol 9 (31) ◽  
pp. 4514-4519 ◽  
Author(s):  
Adel M. Michael ◽  
R. Andrew Shalliker
Keyword(s):  
Ascorbic Acid ◽  
Phase Separation ◽  
Bonded Phase

This work describes two novel validated HPLC methods for the separation and determination of potent antioxidant formulation.

scholarly journals Dual-emission ratio fluorescence for selective and sensitive detection of ferric ions and ascorbic acid based on one-pot synthesis of glutathione protected gold nanoclusters

RSC Advances ◽  
2021 ◽  
Vol 11 (28) ◽  
pp. 17283-17290
Author(s):  
Shuai Zhang ◽  
Cong Zhang ◽  
Xiaodong Shao ◽  
Rentian Guan ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gold Nanoclusters ◽  
Sensitive Detection ◽  
Dual Fluorescence ◽  
Ferric Ions ◽  
Fluorometric Method ◽  
One Pot ◽  
Dual Emission ◽  
One Pot Synthesis

A fluorometric method was proposed for the determination of Fe3+ and ascorbic acid (AA) based on blue and red dual fluorescence emissions of glutathione (GSH) stabilized-gold nanoclusters (AuNCs).

Sign in / Sign up

Export Citation Format

Share Document