Methods for the determination of cyclopropenoid fatty acids. I. Aqueous hydrochloric acid method

1963 ◽  
Vol 40 (12) ◽  
pp. 716-717 ◽  
Author(s):  
F. C. Magne ◽  
J. A. Harris ◽  
E. L. Skau
Keyword(s):  
Fatty Acids ◽  
Hydrochloric Acid ◽  
Acid Method ◽  
Aqueous Hydrochloric Acid ◽  
Cyclopropenoid Fatty Acids

scholarly journals Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 901
Author(s):  
Veronica Lolli ◽  
Pablo G. Toral ◽  
Augusta Caligiani ◽  
Pilar Gómez-Cortés
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Sterculic Acid ◽  
Bioactive Lipids ◽  
Cyclopropenoid Fatty Acids ◽  
Δ9 Desaturase ◽  
First Time ◽  
Endogenous Synthesis

Cyclopropenoid fatty acids (CPEFA), found in oilseeds from Malvaceae and Sterculiaceae, have been shown to interfere with the endogenous synthesis of several bioactive lipids of dairy fat, such as cis-9, trans-11 18:2 and cis-9 18:1, by inhibiting Δ9-desaturase. No previous study has reported the presence of sterculic acid in animal fat and its incorporation in tissues after its administration, due to the lack of a proper methodology. In the present research, a GC-MS method based on cold base derivatization to fatty acids methylesters was developed to determine CPEFA in ewe milk triglycerides, after infusing sterculic acid (0.5 g/day) to six lactating ewes. An alternative derivatization based on silanyzation followed by GC-MS analysis was also tested, showing its possible applicability when CPEFA are present in the form of free fatty acids. Sterculic acid was detected in ewe milk triglycerides, demonstrating its incorporation from the bloodstream into milk by the mammary gland. The mean transfer rate represented 8.0 ± 1.0% of the daily dose. This study provides, for the first time, the presence of sterculic acid in milk fat, supporting the importance of understanding its occurrence in vivo and encouraging further research to determine whether it can be present in foods, such as dairy products, obtained under practical farming conditions.

Determination of Nutritional and Cyclopropenoid Fatty Acids in Cottonseed by a Single GC Analysis

2015 ◽  
Vol 92 (7) ◽  
pp. 947-956 ◽  
Author(s):  
Barb Mitchell ◽  
Brent Rozema ◽  
Thomas Vennard ◽  
Jane Sabbatini
Keyword(s):  
Fatty Acids ◽  
Gc Analysis ◽  
Cyclopropenoid Fatty Acids

Determination of Polysorbate 80 in Bakery Products and Frozen Desserts

1964 ◽  
Vol 47 (4) ◽  
pp. 685-688
Author(s):  
Martha J Hall
Keyword(s):  
Aqueous Solution ◽  
Fatty Acids ◽  
Hydrochloric Acid ◽  
Potassium Hydroxide ◽  
Gravimetric Method ◽  
Phosphomolybdic Acid ◽  
Chloroform Extract ◽  
Polysorbate 80 ◽  
Bakery Products

Abstract A gravimetric method has been developed for determining polysorbate 80 at levels of 0.05 and 0.1% in bread, ice cream, and cake mix. The sample is hydrolyzed and saponified with hydrochloric acid and potassium hydroxide, respectively, and the liberated glycol is extracted with chloroform. The residue from the chloroform extracts is dissolved in water, and the fatty acids are extracted with petroleum ether. The glycol is then re-extracted from the aqueous solution into chloroform. The chloroform extract is evaporated to dryness, and the residue is dissolved in water and precipitated with phosphomolybdic acid. Recoveries at the 0.05% level ranged between 75 and 105%, and at the 0.1% level, between 85 and 107%.

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