Further characterization of the outer membrane ofSerratia marcescens and an oxacilline-sensitive mutant: Surface carbohydrates and outer membrane lipid composition

1979 ◽  
Vol 2 (3) ◽  
pp. 127-130
Author(s):  
Klaus B. Heller
Keyword(s):  
Outer Membrane ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Sensitive Mutant ◽  
Membrane Lipid Composition ◽  
Surface Carbohydrates

scholarly journals Unusual Outer Membrane Lipid Composition of the Gram-negative, Lipopolysaccharide-lacking MyxobacteriumSorangium cellulosumSo ce56

2011 ◽  
Vol 286 (15) ◽  
pp. 12850-12859 ◽  
Author(s):  
Matthias Keck ◽  
Nicolas Gisch ◽  
Hermann Moll ◽  
Frank-Jörg Vorhölter ◽  
Klaus Gerth ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Gram Negative ◽  
Membrane Lipid Composition

scholarly journals Identification of a Stress-Induced Factor of Corynebacterineae That Is Involved in the Regulation of the Outer Membrane Lipid Composition

2009 ◽  
Vol 191 (23) ◽  
pp. 7323-7332 ◽  
Author(s):  
Xavier Meniche ◽  
Cécile Labarre ◽  
Célia de Sousa-d'Auria ◽  
Emilie Huc ◽  
Françoise Laval ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Lipid Composition ◽  
Mycolic Acid ◽  
Membrane Lipid ◽  
Protein Family ◽  
Stress Conditions ◽  
Acid Biosynthesis ◽  
Membrane Lipid Composition ◽  
Mycolic Acids ◽  
Lipid Modifications

ABSTRACT Corynebacterineae are gram-positive bacteria that possess a true outer membrane composed of mycolic acids and other lipids. Little is known concerning the modulation of mycolic acid composition and content in response to changes in the bacterial environment, especially temperature variations. To address this question, we investigated the function of the Rv3802c gene, a gene conserved in Corynebacterineae and located within a gene cluster involved in mycolic acid biosynthesis. We showed that the Rv3802 ortholog is essential in Mycobacterium smegmatis, while its Corynebacterium glutamicum ortholog, NCgl2775, is not. We provided evidence that the NCgl2775 gene is transcriptionally induced under heat stress conditions, and while the corresponding protein has no detectable activity under normal growth conditions, the increase in its expression triggers an increase in mycolic acid biosynthesis concomitant with a decrease in phospholipid content. We demonstrated that these lipid modifications are part of a larger outer membrane remodeling that occurs in response to exposure to a moderately elevated temperature (42°C). In addition to showing an increase in the ratio of saturated corynomycolates to unsaturated corynomycolates, our results strongly suggested that the balance between mycolic acids and phospholipids is modified inside the outer membrane following a heat challenge. Furthermore, we showed that these lipid modifications help the bacteria to protect against heat damage. The NCgl2775 protein and its orthologs thus appear to be a protein family that plays a role in the regulation of the outer membrane lipid composition of Corynebacterineae under stress conditions. We therefore propose to name this protein family the envelope lipids regulation factor (ElrF) family.

scholarly journals Changes in membrane lipid composition in ethanol- and acid-adapted Oenococcus oeni cells: characterization of the cfa gene by heterologous complementation

Microbiology ◽  
2008 ◽  
Vol 154 (9) ◽  
pp. 2611-2619 ◽  
Author(s):  
Cosette Grandvalet ◽  
Juan Simón Assad-García ◽  
Son Chu-Ky ◽  
Marie Tollot ◽  
Jean Guzzo ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Oenococcus Oeni ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Heterologous Complementation

scholarly journals Dewetting-induced formation and mechanical properties of synthetic bacterial outer membrane models (GUVs) with controlled inner-leaflet lipid composition

Soft Matter ◽  
2019 ◽  
Vol 15 (19) ◽  
pp. 3938-3948 ◽  
Author(s):  
Sepehr Maktabi ◽  
Jeffrey W. Schertzer ◽  
Paul R. Chiarot
Keyword(s):  
Mechanical Properties ◽  
Outer Membrane ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Giant Unilamellar Vesicles ◽  
Gram Negative ◽  
Membrane Lipid Composition ◽  
Bacterial Outer Membrane ◽  
Membrane Models ◽  
Microfluidic Technique

We report on a microfluidic technique for fabricating monodisperse asymmetric giant unilamellar vesicles (GUVs) possessing the Gram-negative bacterial outer membrane lipid composition.

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