Effects of insulin and glucose on subcellular fractions from rat adipose tissue

1974 ◽  
Vol 11 (3) ◽  
pp. 206-212
Author(s):  
Enrique Pimentel ◽  
Carlos A. González ◽  
Freddy González-Mujica
Keyword(s):  
Adipose Tissue ◽  
Subcellular Fractions ◽  
Rat Adipose Tissue

BIOCHEMICAL EFFECTS OF INSULIN ON SUBCELLULAR FRACTIONS FROM RAT ADIPOSE TISSUE

1973 ◽  
Vol 71 (4_Suppl) ◽  
pp. S121 ◽  
Author(s):  
E. Pimentel ◽  
C. A. Gonzalez ◽  
F. Gonzalez
Keyword(s):  
Adipose Tissue ◽  
Subcellular Fractions ◽  
Biochemical Effects ◽  
Rat Adipose Tissue

scholarly journals Properties of phosphatidate phosphohydrolase in rat adipose tissue

1994 ◽  
Vol 301 (3) ◽  
pp. 793-799 ◽  
Author(s):  
S C Jamdar ◽  
W F Cao
Keyword(s):  
Adipose Tissue ◽  
Plasma Membranes ◽  
Lipid A ◽  
Mesangial Cell ◽  
Intraperitoneal Administration ◽  
Subcellular Fractions ◽  
Phosphatidate Phosphohydrolase ◽  
Membrane Bound ◽  
Rat Adipose Tissue ◽  
Triton X

Previously we have identified the presence of two different phosphatidate phosphohydrolase (PPH) activities in rat adipose tissue, based on Mg(2+)-dependency. In the present investigation, we have further characterized these isoenzymes, using both aqueous dispersed and membrane-bound phosphatidate as substrates and differentiated these activities on the basis of both Mg(2+)-dependency and N-ethylmaleimide (NEM)-sensitivity. These two distinguishing criteria gave identical estimates of PPH activities present in the different subcellular fractions. The microsomal and cytosol fractions contained mainly the Mg(2+)-dependent (NEM-sensitive) form, which was inhibited by various thiol reagents, was inactivated by heating at 55 degrees C for 20 min, and was decreased significantly within 2 h after intraperitoneal administration of cystamine (200 mg/kg). Such treatments had no effects on the Mg(2+)-independent (NEM-insensitive) form of PPH, which was mainly located in the plasma membranes, mitochondrial and microsomal fractions. Addition of Lipid A and guanosine 5′-[gamma-thio]triphosphate to the assay mixture had no effect on the PPH activities. The Mg(2+)-independent PPH form, which was thermostable in the intact subcellular fractions, became thermolabile when these fractions were disrupted in the presence of Triton X-100. The present studies demonstrate that: (1) the thermostability is not a satisfactory index to differentiate these isoenzymes; (2) the thiol/disulphide exchange may be involved in the regulation of Mg(2+)-dependent PPH activity; and (3) the PPH isoenzymes do not seem to be under G-protein control in adipose tissue, as reported previously in the mesangial cell line.

scholarly journals Effects of age and cell size on rat adipose tissue metabolism.

1975 ◽  
Vol 16 (6) ◽  
pp. 461-464 ◽  
Author(s):  
G Holm ◽  
B Jacobsson ◽  
P Björntorp ◽  
U Smith
Keyword(s):  
Adipose Tissue ◽  
Cell Size ◽  
Adipose Tissue Metabolism ◽  
Tissue Metabolism ◽  
Rat Adipose Tissue
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