Interstitial fluid concentration of metabolites in isolated perfused rat hearts as function of myocardial metabolism and capillary permeability

1982 ◽  
Vol 394 (S1) ◽  
pp. R11-R11 ◽  
Author(s):  
B. Wendtland ◽  
E. Jüngling ◽  
H. Kammermeier
Keyword(s):  
Interstitial Fluid ◽  
Myocardial Metabolism ◽  
Capillary Permeability ◽  
Rat Hearts ◽  
Fluid Concentration ◽  
Perfused Rat Hearts ◽  
Isolated Perfused Rat Hearts

ATP as a source of interstitial adenosine in the rat heart

1999 ◽  
Vol 77 (8) ◽  
pp. 579-588 ◽  
Author(s):  
Mojca Lorbar ◽  
Richard A Fenton ◽  
James G Dobson, Jr.
Keyword(s):  
Electrical Stimulation ◽  
Interstitial Fluid ◽  
Contractile Function ◽  
Stellate Ganglion ◽  
Perfused Heart ◽  
Reserpine Treatment ◽  
Rat Hearts ◽  
Perfused Rat Hearts ◽  
Stimulation Of ◽  
Isolated Perfused Rat Hearts

The contribution of neuronal ATP to interstitial adenosine levels was investigated in isolated perfused rat hearts. Ventricular surface transudates, representing interstitial fluid, were analyzed for norepinephrine, ATP, and adenosine. Exocytotic release of norepinephrine was induced by electrical stimulation of cardiac efferents emanating from the stellate ganglion. Ganglion stimulation increased contractility, interstitial norepinephrine, ATP, and adenosine. Interstitial adenosine was 11- to 27-fold higher than interstitial ATP, suggesting that the released ATP is unlikely the only source of adenosine. In the presence of AOPCP (α,β-methyleneadenosine 5'-diphosphate), an ecto-5'-nucleotidase inhibitor, the ganglion-stimulated increase in interstitial ATP and adenosine reached levels similar to those in the absence of AOPCP, also suggesting that adenosine does not derive from extracellular ATP. The perfusate Ca2+ was raised from 1 to 4 mM to determine the importance of the enhanced contractile function on the levels of norepinephrine, ATP, and adenosine. The results were increases in contractility and interstitial norepinephrine, ATP, and adenosine, which were not suppressed with atenolol, indicating a norepinephrine-independent release of ATP and adenosine. Reserpine treatment and administration of guanethidine depleted the catecholamine stores and diminished the catecholamine release, respectively. However, neither agent altered Ca2+-induced increases in ATP and adenosine. It is concluded that the amount of neuronal-derived ATP is low and most likely does not contribute significantly to interstitial levels of adenosine. Furthermore, elevations in interstitial norepinephrine, ATP, and adenosine are associated with neuronal-independent increases in contractile function.Key words: perfused heart, stellate ganglion, co-transmission, calcium, and contractility.

Intra- and extracellular markers in interstitial transudate of perfused rat hearts

1988 ◽  
Vol 254 (4) ◽  
pp. H785-H794
Author(s):  
W. Wienen ◽  
H. Kammermeier
Keyword(s):  
Capillary Permeability ◽  
Molecular Weights ◽  
Rat Hearts ◽  
Arterial Concentration ◽  
Perfused Rat Hearts ◽  
O2 Supply ◽  
Kinetics Of ◽  
Control State ◽  
Isolated Perfused Rat Hearts

The interstitial transudate was investigated in isolated perfused rat hearts. Capillary permeability and the kinetics of interstitial uptake and release were characterized using four different marker molecules (mol wt 522 to 2 X 10(6)). The half-time (t1/2) values (less than 30 to 170 s) and the interstitial concentration after 30 min (100-44% of arterial concentration) reflected the order and inverse order of their molecular weights, respectively. Creatine kinase (CK) and glutathione (GSH) were measured during control state, hypoxia, and anoxia, followed by reoxygenation. Interstitial concentrations of CK and GSH were higher by a factor of 100 and 8, respectively, compared with the venous effluent. During hypoxia (PO2 = 110 mmHg, i.e., O2 supply = 30% of demand) and reoxygenation there was a significant increase only in the interstitial (not venous) release of CK and GSH, which was further increased during anoxia. Ischemia (75 min) and reperfusion cause no interstitial release of lysosomal (acid phosphatase) and mitochondrial (glutamate dehydrogenase) enzymes despite a massive loss of cytosolic enzymes. Examination of the interstitial transudate allows characterization of capillary transfer and provides a very sensitive measure of sarcolemmal release phenomena.

Chaotic Heart Rate Dynamics in Isolated Perfused Rat Hearts

1989 ◽  
pp. 215-224 ◽  
Author(s):  
Joseph P. Zbilut ◽  
Gottfried Mayer-Kress ◽  
Paul A. Sobotka ◽  
Michael O’Toole ◽  
John X. Thomas
Keyword(s):  
Heart Rate ◽  
Rat Hearts ◽  
Heart Rate Dynamics ◽  
Perfused Rat Hearts ◽  
Isolated Perfused Rat Hearts
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