Does the Kensey catheter keep a coaxial position inside the arterial lumen?: An in-vitro angioscopic study

1991 ◽  
Vol 14 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Abdurrazzak Abdulkader Gehani ◽  
Alban Davies ◽  
Keith Stoodley ◽  
Simon Ashley ◽  
Stephen G. Brook ◽  
...  
Keyword(s):  
Arterial Lumen ◽  
Kensey Catheter

scholarly journals Endotoxinemia Accelerates Atherosclerosis via Electrostatic Charge-Mediated Monocyte Adhesion

Circulation ◽  
2020 ◽  
Author(s):  
Ariane Schumski ◽  
Almudena Ortega-Gómez ◽  
Kanin Wichapong ◽  
Carla Winter ◽  
Patricia Lemnitzer ◽  
...  
Keyword(s):  
Vascular Inflammation ◽  
Acute Infection ◽  
Atherosclerotic Lesion ◽  
Myeloid Cell ◽  
Lesion Size ◽  
Histone H2a ◽  
Monocyte Adhesion ◽  
Arterial Lumen ◽  
Lps Treatment

Background: Acute infection is a well-established risk factor of cardiovascular inflammation increasing the risk for a cardiovascular complication within the first weeks after infection. However, the nature of the processes underlying such aggravation remains unclear. Lipopolysaccharide (LPS) derived from Gram-negative bacteria is a potent activator of circulating immune cells including neutrophils, which foster inflammation through discharge of neutrophil extracellular traps (NETs). Here we utilize a model of endotoxinemia to link acute infection and subsequent neutrophil activation with acceleration of vascular inflammation. Methods: Acute infection was mimicked by injection of a single dose of LPS into hypercholesterolemic mice. Atherosclerosis burden was studied by histomorphometric analysis of the aortic root. Arterial myeloid cell adhesion was quantified by intravital microscopy. Results: LPS treatment rapidly enhanced atherosclerotic lesion size by expansion of the lesional myeloid cell accumulation. LPS treatment led to the deposition of NETs along the arterial lumen and inhibition of NET release annulled lesion expansion during endotoxinemia, thus suggesting that NETs regulate myeloid cell recruitment. To study the mechanism of monocyte adhesion to NETs, we employed in vitro adhesion assays and biophysical approaches. In these experiments, NET-resident histone H2a attracted monocytes in a receptor-independent, surface charge-dependent fashion. Therapeutic neutralization of histone H2a by antibodies or by in silico designed cyclical peptides enables us to reduce luminal monocyte adhesion and lesion expansion during endotoxinemia. Conclusions: Our study shows, that NET-associated histone H2a mediates charge-dependent monocyte adhesion to NETs and accelerates atherosclerosis during endotoxinemia.

scholarly journals Experimental Polyvinyl Alcohol Core Coil for a Drug Delivery System

2003 ◽  
Vol 9 (1_suppl) ◽  
pp. 107-111 ◽  
Author(s):  
H. Matsumoto ◽  
T. Terada ◽  
M. Tsuura ◽  
T. Itakura ◽  
A. Ogawa
Keyword(s):  
Electron Microscopy ◽  
Carotid Artery ◽  
Polyvinyl Alcohol ◽  
Common Carotid Artery ◽  
Cellular Proliferation ◽  
Neointimal Hyperplasia ◽  
Cellular Adhesion ◽  
Biologically Active ◽  
Arterial Lumen

We developed a new type of coil with a polyvinyl alcohol core (PVA-core coil) to absorb and release various types of biologically active materials, for the endovascular treatment of intracranial aneurysms. A 10 mm segment of the PVA-core coil was used in this study. PVA-core coils were immersed in basic fibroblast growth factor (b-FGF) solution. The PVA-core coil, which absorbed b-FGF in the PVA core, was named FGF-core coil. This coil gradually released b-FGF in the solution without b-FGF. In vitro study, FGF-core coils, PVA-core coils and unmodified coils were cultured with fibroblasts (NIH3T3) respectively and their surface was observed with scanning electron microscopy (SEM). In vivo study, each coils were inserted into the rat common carotid artery. Rats were sacrificed and the arterial lumen were histologically examined 14 days and 28 days after coil implantation. Electron microscopy findings demonstrated remarkable cellular adhesion to the surface of the FGF-core coils, while no adhesion to the surface of the PVA-core coils and unmodified coils was found. Histologically, remarkable cellular proliferation and wall thickness like neointimal hyperplasia was demonstrated in the implanted common carotid artery of the FGF-core coil group at 14 days and 28 days. On the other hand, these changes did not occur in PVA-core coil group and unmodified coil group. We suggest that FGF-core coils may be effective to induce fibrotic changes inside cerebral aneurysms.

In Vitro Contact Lithotripsy of Gallstones with the Kensey Catheter

1989 ◽  
Vol 24 (12) ◽  
pp. S124
Author(s):  
T Ruder ◽  
E Omessi ◽  
GR Wittich ◽  
D Swanson ◽  
A Wright
Keyword(s):  
Kensey Catheter

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

Sign in / Sign up

Export Citation Format

Share Document