Vitamin D-dependent active calcium transport: The role of CaBP

1988 ◽  
Vol 43 (3) ◽  
pp. 133-137 ◽  
Author(s):  
Felix Bronner
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Active Calcium

scholarly journals Analysis of 1,25-Dihydroxyvitamin D3 Genomic Action Reveals Calcium-Regulating and Calcium-Independent Effects in Mouse Intestine and Human Enteroids

2020 ◽  
Vol 41 (1) ◽  
pp. e00372-20
Author(s):  
Shanshan Li ◽  
Jessica De La Cruz ◽  
Steven Hutchens ◽  
Somshuvra Mukhopadhyay ◽  
Zachary K. Criss ◽  
...  
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Target Genes ◽  
Dihydroxyvitamin D3 ◽  
Transgenic Expression ◽  
Distal Intestine ◽  
Dihydroxyvitamin D ◽  
Active Calcium ◽  
Independent Effects ◽  
Proximal Intestine

ABSTRACTAlthough vitamin D is critical for the function of the intestine, most studies have focused on the duodenum. We show that transgenic expression of the vitamin D receptor (VDR) only in the distal intestine of VDR null mice (KO/TG mice) results in the normalization of serum calcium and rescue of rickets. Although it had been suggested that calcium transport in the distal intestine involves a paracellular process, we found that the 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-activated genes in the proximal intestine associated with active calcium transport (Trpv6, S100g, and Atp2b1) are also induced by 1,25(OH)2D3 in the distal intestine of KO/TG mice. In addition, Slc30a10, encoding a manganese efflux transporter, was one of the genes most induced by 1,25(OH)2D3 in both proximal and distal intestine. Both villus and crypt were found to express Vdr and VDR target genes. RNA sequence (RNA-seq) analysis of human enteroids indicated that the effects of 1,25(OH)2D3 observed in mice are conserved in humans. Using Slc30a10−/− mice, a loss of cortical bone and a marked decrease in S100g and Trpv6 in the intestine was observed. Our findings suggest an interrelationship between vitamin D and intestinal Mn efflux and indicate the importance of distal intestinal segments to vitamin D action.

Calcium transport in small intestine during early development: role of vitamin D

1980 ◽  
Vol 239 (6) ◽  
pp. G473-G479 ◽  
Author(s):  
B. P. Halloran ◽  
H. F. DeLuca
Keyword(s):  
Vitamin D ◽  
Small Intestine ◽  
Early Development ◽  
Calcium Transport ◽  
Plasma Concentrations ◽  
Suckling Period ◽  
Dihydroxyvitamin D3 ◽  
Weanling Rats ◽  
Normal Males

To define the role of vitamin D in calcium transport in the intestine during early development, female weanling rats were placed on vitamin D-replete or vitamin D-deficient diets, grown to maturity, and mated with normal males. Pups born to vitamin D-replete and vitamin D-deficient mothers were killed at various times after parturition, and calcium transport in the small intestine as well as the concentrations of calcium and phosphate in the plasma were measured. Transport of calcium in pups from vitamin D-replete and vitamin D-deficient litters was identical at 3 and 14 days postpartum but was threefold greater in pups from vitamin D-replete litters at weaning and 3 wk postweaning. 1,25-dihydroxyvitamin D3 had no effect on calcium transport at 14 days postpartum but did induce transport at weaning. Plasma concentrations of calcium at 3 days postpartum were nearly normal but decreased during the suckling period from 10.3 mg/100ml to 7.2 mg/100 ml in vitamin D-deficient rats. These results suggest that calcium transport in the intestine during early development is not mediated by vitamin D but that a vitamin D-sensitive transport system develops late in the suckling period.

The role of vitamin D in chorioallantoic membrane calcium transport

1994 ◽  
Vol 1192 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Mahmoud A. Elaroussi ◽  
Ann Uhland-Smith ◽  
Wendy Hellwig ◽  
Hector F. DeLuca
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Chorioallantoic Membrane

Calcium transport in small intestine during pregnancy and lactation

1980 ◽  
Vol 239 (1) ◽  
pp. E64-E68 ◽  
Author(s):  
B. P. Halloran ◽  
H. F. DeLuca
Keyword(s):  
Vitamin D ◽  
Reproductive Cycle ◽  
Calcium Transport ◽  
Plasma Concentrations ◽  
Dihydroxyvitamin D ◽  
Control Value ◽  
1,25 Dihydroxyvitamin D ◽  
Everted Gut Sac ◽  
Pregnancy And Lactation ◽  
Active Calcium

The factors involved in calcium homeostasis during the mammalian reproductive cycle and specifically in the control of active calcium transport in the intestine have not been thoroughly investigated. For this reason calcium transport in the intestine was measured in vitamin D-replete and vitamin D-deficient rats during pregnancy and lactation using the everted gut sac technique. In addition the changes in the plasma concentrations of calcium and 1,25-dihydroxyvitamin D were measured and correlated with transport. During the later stages of pregnancy and during lactation, the concentration of calcium in the plasma is reduced 10-30%. In turn, in the vitamin D-replete rat, the concentration of 1,25-dihydroxyvitamin D in the plasma increases from a control value of 26 pg/ml to 158 pg/ml at day 14 of lactation. Calcium transport in the intestine increases late in pregnancy, peaks during lactation, and then falls to control values by 3 wk postweaning in both vitamin D-replete and D-deficient animals. These findings strengthen the established relationship between 1,25-dihydroxyvitamin D and active calcium transport in the intestine as well as suggest that some factor(s) independent of vitamin D is stimulating intestinal calcium transport during the reproductive cycle.

Vitamin D and Magnesium Absorption in Man

1979 ◽  
Vol 57 (1) ◽  
pp. 121-123 ◽  
Author(s):  
A. Hodgkinson ◽  
D. H. Marshall ◽  
B. E. C. Nordin
Keyword(s):  
Vitamin D ◽  
Calcium Transport ◽  
Alkaline Earth ◽  
Calcium Balance ◽  
Slight Effect ◽  
Strontium Barium ◽  
Active Calcium ◽  
The Mean ◽  
Calcium And Magnesium ◽  
Stimulation Of

1. The effects of vitamin D and its hydroxylated derivatives on calcium and magnesium absorption have been examined in 47 balance studies on patients with various disorders of calcium or bone metabolism. 2. Vitamin D significantly increased the mean net absorption of calcium and also the calcium balance. The mean net absorption of magnesium was also significantly increased although the rise was much less than that of calcium and the mean magnesium balance was unaffected. 3. It is suggested that the slight effect of vitamin D on magnesium absorption may be incidental to its stimulation of active calcium transport, since the latter system has weak affinities for other alkaline earth ions including strontium, barium and magnesium.

Localization of vitamin D-dependent active Ca2+ transport in rat duodenum and relation to CaBP

1986 ◽  
Vol 251 (3) ◽  
pp. G314-G320 ◽  
Author(s):  
C. Roche ◽  
C. Bellaton ◽  
D. Pansu ◽  
A. Miller ◽  
F. Bronner
Keyword(s):  
Vitamin D ◽  
Brush Border ◽  
Calcium Binding ◽  
Calcium Transport ◽  
Positive Function ◽  
Extrusion Process ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Rat Duodenum ◽  
Active Calcium

Vitamin D-replete (+D) and vitamin D-deficient (-D) rats received by intraperitoneal injection varying amounts of 1,25-dihydroxyvitamin D3, and 4 h (+D) or 9 h (-D) later everted duodenal sacs were prepared to evaluate active calcium transport, i.e., the amount of calcium found in the serosal fluid. At the same time, duodenal calcium-binding protein (CaBP) content was measured. Calcium transport was a close positive function of CaBP content. It was not detectable when CaBP content was zero and increased linearly without plateauing as CaBP content increased to 100 nmol calcium bound/g mucosa. Trifluoperazine (TFP) inhibited active calcium transport in a concentration-dependent manner. Experiments using vesicles prepared from brush-border or basolateral membranes indicated that TFP inhibited the calcium-extrusion process, with virtually no effect on calcium entry. It is concluded that vitamin D exerts its major regulation of active calcium transport in the rat duodenum via CaBP on transport steps beyond brush-border entry.

Vitamin D and permeability of intestinal mucosa to calcium

1965 ◽  
Vol 208 (2) ◽  
pp. 370-374 ◽  
Author(s):  
Harold E. Harrison ◽  
Helen C. Harrison
Keyword(s):  
Vitamin D ◽  
Intestinal Mucosa ◽  
Diffusion Barrier ◽  
Calcium Transport ◽  
Transport Systems ◽  
Mucosal Epithelium ◽  
Dependent Transport ◽  
Energy Dependent

The role of the permeability of intestinal mucosa to calcium was determined in rats by comparison of calcium transport in vitro across the wall of everted intestinal loops from which the mucosal epithelium was removed and of loops with intact epithelium. Energy-dependent transport systems were inhibited by incubation at 5 C or by addition of N-ethyl maleimide to the medium. The experiments were designed to measure calcium transport along a concentration gradient between mucosal and serosal surfaces at varying concentrations of calcium in the mucosal solution. The results indicate that the intact intestinal mucosa presents a diffusion barrier to calcium and that this diffusion barrier is lessened by vitamin D treatment of the animal from which the intestine is obtained.

Influence of vitamin D on calcium absorption in the chick

1962 ◽  
Vol 203 (3) ◽  
pp. 497-505 ◽  
Author(s):  
J. D. Sallis ◽  
E. S. Holdsworth
Keyword(s):  
Vitamin D ◽  
Small Intestine ◽  
Calcium Transport ◽  
Calcium Absorption ◽  
Metabolic Inhibitors ◽  
Hydroxyl Groups ◽  
Oxidation Reduction ◽  
Active Calcium

The site of absorption of Ca45 was studied in rachitic chicks and rachitic chicks given vitamin D3. Vitamin D3 markedly increases absorption from the small intestine and, in vivo, similar amounts of calcium are absorbed along the entire small intestine. With everted gut sacs, the distal third of the small intestine transported much more calcium than did the duodenal and middle sections. Thus, interpretations of in vitro results may not always depict the natural in vivo process. Vitamin D2 had little activity in the chick, but AT-10 series 2 and AT-10 series 3 were almost as active as vitamin D3 for calcium transport. These results suggest an "active carrier" may be formed by addition of hydrogen or hydroxyl groups to the opened ring B of vitamin D, giving a carrier capable of reversible oxidation-reduction or keto-enol tautomerism. Using metabolic inhibitors, active calcium transport in vitro relied on glycolysis for its energy supply. The transport was independent of the sodium pump.

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