Effects of vitamin D-binding protein on bone resorption stimulated by 1,25 dihydroxyvitamin D3

1990 ◽  
Vol 47 (3) ◽  
pp. 164-168 ◽  
Author(s):  
Socorro Vargas ◽  
Roger Bouillon ◽  
Hugo Van Baelen ◽  
Lawrence G. Raisz
Keyword(s):  
Vitamin D ◽  
Bone Resorption ◽  
Binding Protein ◽  
Vitamin D Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

scholarly journals Influence of the Vitamin D-binding Protein on the Serum Concentration of 1,25-Dihydroxyvitamin D3

1981 ◽  
Vol 67 (3) ◽  
pp. 589-596 ◽  
Author(s):  
Roger Bouillon ◽  
Frans A. Van Assche ◽  
Hugo Van Baelen ◽  
Walter Heyns ◽  
Pieter De Moor
Keyword(s):  
Vitamin D ◽  
Serum Concentration ◽  
Binding Protein ◽  
Vitamin D Binding Protein ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

scholarly journals Cellular Expression Levels of the Vitamin D Receptor Are Critical to Its Transcriptional Regulation by the Pituitary Transcription Factor Pit-1

2007 ◽  
Vol 21 (7) ◽  
pp. 1513-1525 ◽  
Author(s):  
Samuel Seoane ◽  
Isabel Ben ◽  
Viviana Centeno ◽  
Roman Perez-Fernandez
Keyword(s):  
Vitamin D ◽  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Vitamin D Receptor ◽  
Binding Protein ◽  
Target Cells ◽  
Dihydroxyvitamin D3 ◽  
Expression Levels ◽  
1,25 Dihydroxyvitamin D3 ◽  
Mcf 7

Abstract The biological role of 1,25-dihydroxyvitamin D3 has generally been related to calcium homeostasis, but this hormone also has fundamental effects on processes of cellular proliferation and differentiation. The genomic actions of 1,25-dihydroxyvitamin D3 are mediated by the vitamin D receptor (VDR) present in target cells. However, VDR transcriptional regulation is not well understood, probably attributable to the complexity of the VDR gene and its promoter. In the present study, it is demonstrated that administration of the pituitary transcription factor Pit-1 (originally found in the pituitary gland but also present in other nonpituitary cell types and tissues) to the MCF-7 (human breast adenocarcinoma) cell line induces a significant increase in VDR mRNA and protein levels. Conversely, Pit-1-targeted small interference RNA markedly reduced expression of VDR in MCF-7 cells. Reporter gene assays demonstrated that the effect of Pit-1 is mediated by its binding to a region located between −254 and −246 bp from the VDR transcription start site. Selective mutations of this site completely abolished VDR transcription. Chromatin immunoprecipitation analysis showed that binding of Pit-1 to the VDR promoter leads additionally to recruitment of cAMP response element-binding protein binding protein, acetylated histone H4, and RNA polymerase II. Surprisingly, Pit-1 binding also recruits VDR protein to the VDR promoter. Using several cell lines with different levels of VDR expression, it was demonstrated that up-regulation of VDR transcription by Pit-1 is dependent on the presence of VDR protein, suggesting that transcriptional expression of VDR in a given cell type is dependent on, among other factors, its own expression levels.

Profile of ligand specificity of the vitamin D binding protein for 1α,25-dihydroxyvitamin d3 and its analogs

2009 ◽  
Vol 9 (8) ◽  
pp. 1277-1288 ◽  
Author(s):  
June E. Bishop ◽  
Elaine D. Collins ◽  
William H. Okamura ◽  
Anthony W. Norman
Keyword(s):  
Vitamin D ◽  
Binding Protein ◽  
Ligand Specificity ◽  
Vitamin D Binding Protein ◽  
Dihydroxyvitamin D3

scholarly journals 1,25-Dihydroxyvitamin D3 modulates adipogenesis of human adipose-derived mesenchymal stem cells dose-dependently

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Amin Salehpour ◽  
Mehdi Hedayati ◽  
Farzad Shidfar ◽  
Asal Neshatbini Tehrani ◽  
Ali Asghar Farshad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vitamin D ◽  
Mesenchymal Stem Cells ◽  
Fatty Acid ◽  
Binding Protein ◽  
Adipogenic Differentiation ◽  
Dependent Manner ◽  
Dihydroxyvitamin D3 ◽  
Fatty Acid Binding ◽  
1,25 Dihydroxyvitamin D3

Abstract Purpose 1,25-dihydroxyvitamin D3 may regulate adipogenesis in adipocytes in-vitro, but little is known about possible molecular mechanisms related to the inhibitory effect of 1,25-dihydroxyvitamin D3 on adipogenesis in humans҆ adipose tissue. Methodology In this study, human adipose-derived mesenchymal stem cells (hASCs) were cultured for 14 days in adipogenic differentiation media containing concentrations of 1,25-dihydroxyvitamin D3 (10−10–10−8 M). The extent of adipogenic differentiation in ASCs was assessed by Oil Red O staining and quantitative polymerase chain reaction (PCR) to determine expression levels of key adipogenic markers. Results Our results showed that vitamin D receptor (VDR), as a mediator of most actions of 1,25-dihydroxyvitamin D3, glucose trasporter-4 (GLUT4),and fatty acid binding protein-4 (FABP4) was expressed in vitamin D-treated hASCs. However, the protein level of these markers was lower than the control group. Treatment of human preadipocytes with 1,25-dihydroxyvitamin D3 significantly altered expression of adipogenic markers and triglyceride accumulation in a dose-dependent manner. 1,25-dihydroxyvitamin D3 at concentration of 10−8 M enhanced expression of sterol regulatory element-binding protein-1c (SREBP1c), CCAAT-enhancer-binding protein-β (C/EBPβ), a mitotic clonal expansion, peroxisome proliferator-activated receptor-gamma (PPARγ), fatty acid synthase (FASN), a marker of de novo lipogenesis,and lipoprotein lipase (LPL). Conclusion Our findings revealed that 1,25-dihydroxyvitamin D3 may provoke adipocyte development in critical periods of adipogenesis at concentration of 10−8 M, thereby leading to a greater risk of obesity in adulthood and an augmented risk of obesity-related diseases including diabetes, cardiovascular diseases, and some cancers.

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