Cyclic AMP-dependent membrane protein phosphorylation and calmodulin binding are involved in the rapid stimulation of muscle calcium uptake by 1,25-dihydroxyvitamin D3

1990 ◽  
Vol 47 (5) ◽  
pp. 314-319 ◽  
Author(s):  
Luis M. Fernandez ◽  
Virginia Massheimer ◽  
Ana R. de Boland
Keyword(s):  
Cyclic Amp ◽  
Membrane Protein ◽  
Protein Phosphorylation ◽  
Calcium Uptake ◽  
Dihydroxyvitamin D3 ◽  
Calmodulin Binding ◽  
1,25 Dihydroxyvitamin D3 ◽  
Rapid Stimulation ◽  
Muscle Calcium ◽  
Stimulation Of

scholarly journals Modulation of 1,25-dihydroxyvitamin D3-dependent Ca2+ uptake in skeletal muscle by protein kinase C

1992 ◽  
Vol 281 (2) ◽  
pp. 349-352 ◽  
Author(s):  
V Massheimer ◽  
A R de Boland
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Short Exposure ◽  
Dihydroxyvitamin D3 ◽  
45Ca Uptake ◽  
1,25 Dihydroxyvitamin D3 ◽  
Kinase C ◽  
Stimulation Of

In vitro studies have shown that short exposure (1-10 min) of vitamin D-deficient chick soleus muscle to 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] causes an acute stimulation of tissue 45Ca uptake through voltage-gated Ca2+ channels, with parallel increases in cyclic AMP levels, adenylate cyclase activity and membrane protein phosphorylation. We further investigated the involvement of protein kinases in the rapid effects of 1,25(OH)2D3 on skeletal muscle. The hormone was found to stimulate the protein kinase C (PKC) activity of muscle membranes. The PKC activator phorbol 12-myristate 13-acetate (PMA, 100 nM) was found to rapidly stimulate muscle 45Ca uptake, mimicking 1,25(OH)2D3. Increases of 68% and 46% were observed at 1 and 15 min of exposure to PMA respectively. The effects of PMA were dose-dependent (50-200 nM) and were specific, since the inactive analogue 4 alpha-phorbol was without effect. Analogously to the effects of the sterol, PMA-enhanced 45Ca uptake was abolished by the Ca2+ channel antagonists nifedipine (30 microM) and verapamil (50 microM). Staurosporine (10 nM), a PKC inhibitor, surprisingly potentiated 1,25(OH)2D3-dependent stimulation of 45Ca uptake. Exposure of skeletal muscle to PMA (100 nM) plus 1,25(OH)2D3 (1 nM) produced a less pronounced effect on 45Ca uptake than either agent alone. PMA also decreased muscle cyclic AMP levels. These results suggest a regulatory link between the two major transmembrane signalling systems in the mechanism of action of 1,25(OH)2D3 in skeletal muscle.

Stimulation of calcium uptake in cultured astrocytes by hypoosmotic stress — effect of cyclic AMP

1994 ◽  
Vol 645 (1-2) ◽  
pp. 27-35 ◽  
Author(s):  
Alex S. Bender ◽  
Lily L. Mantelle ◽  
Michael D. Norenberg
Keyword(s):  
Cyclic Amp ◽  
Calcium Uptake ◽  
Stress Effect ◽  
Cultured Astrocytes ◽  
Hypoosmotic Stress ◽  
Stimulation Of
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