Sesamin is a potent and specific inhibitor of Δ5 desaturase in polyunsaturated fatty acid biosynthesis

Lipids ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 512-516 ◽  
Author(s):  
Sakayu Shimizu ◽  
Kengo Akimoto ◽  
Yoshifumi Shinmen ◽  
Hiroshi Kawashima ◽  
Michihiro Sugano ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Specific Inhibitor ◽  
Acid Biosynthesis ◽  
Δ5 Desaturase

Inhibition of Δ5-desaturase in polyunsaturated fatty acid biosynthesis by (−)-asarinin and (−)-epiasarinin

1992 ◽  
Vol 31 (3) ◽  
pp. 757-760 ◽  
Author(s):  
Sakayu Shimizu ◽  
Hiroshi Kawashima ◽  
Kengo Akimoto ◽  
Yoshifumi Shinmen ◽  
Hiromichi Sugano ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Δ5 Desaturase

Polyunsaturated fatty acid biosynthesis in human CD3+ T cells is temporally related to cell activation.

2021 ◽  
Author(s):  
Annette West ◽  
Karen Lilycrop ◽  
Philip Calder ◽  
Barbara Fielding ◽  
Johanna Von Gerichten ◽  
...  
Keyword(s):  
T Cells ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Cell Activation ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis

scholarly journals The same rat Δ6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis

2002 ◽  
Vol 364 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Sabine D'ANDREA ◽  
Hervé GUILLOU ◽  
Sophie JAN ◽  
Daniel CATHELINE ◽  
Jean-Noël THIBAULT ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Biosynthesis ◽  
Chain Polyunsaturated Fatty Acid ◽  
Acid Biosynthesis ◽  
Highly Unsaturated Fatty Acids ◽  
Long Chain ◽  
Δ6 Desaturase

The recently cloned Δ6-desaturase is known to catalyse the first step in very-long-chain polyunsaturated fatty acid biosynthesis, i.e. the desaturation of linoleic and α-linolenic acids. The hypothesis that this enzyme could also catalyse the terminal desaturation step, i.e. the desaturation of 24-carbon highly unsaturated fatty acids, has never been elucidated. To test this hypothesis, the activity of rat Δ6-desaturase expressed in COS-7 cells was investigated. Recombinant Δ6-desaturase expression was analysed by Western blot, revealing a single band at 45kDa. The putative involvement of this enzyme in the Δ6-desaturation of C24:5n-3 to C24:6n-3 was measured by incubating transfected cells with C22:5n-3. Whereas both transfected and non-transfected COS-7 cells were able to synthesize C24:5n-3 by elongation of C22:5n-3, only cells expressing Δ6-desaturase were also able to produce C24:6n-3. In addition, Δ6-desaturation of [1-14C]C24:5n-3 was assayed invitro in homogenates from COS-7 cells expressing Δ6-desaturase or not, showing that Δ6-desaturase catalyses the conversion of C24:5n-3 to C24:6n-3. Evidence is therefore presented that the same rat Δ6-desaturase catalyses not only the conversion of C18:3n-3 to C18:4n-3, but also the conversion of C24:5n-3 to C24:6n-3. A similar mechanism in the n-6 series is strongly suggested.

Inhibition of de novo Fatty-Acid Biosynthesis in Isolated Chloroplasts by the Antibiotic Cerulenin and Its Synthetic Derivatives

1992 ◽  
Vol 47 (5-6) ◽  
pp. 382-386 ◽  
Author(s):  
Bernd List ◽  
Andrea Golz ◽  
Wilhelm Boland ◽  
Hartmut K. Lichtenthaler
Keyword(s):  
Fatty Acid ◽  
Inhibitory Activity ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Specific Inhibitor ◽  
Hydrocarbon Chain ◽  
Acetate Incorporation ◽  
Acid Biosynthesis ◽  
Structural Element ◽  
Isolated Chloroplasts

The antibiotic cerulenin was shown to be a potent dose-dependent inhibitor of de novo fattyacid biosynthesis in intact isolated chloroplasts of different plants (measured as [14C]acetate incorporation into the total fatty-acid fraction). Various chemical derivatives of cerulenin were synthesized and tested in the chloroplast assay-system of oat, spinach and pea. Modifications of the hydrocarbon chain of cerulenin (e.g. tetrahydro-cerulenin and its short-chain cis-2,3-epoxy-4-oxoheptanamide derivative) decreased the inhibitory activity of cerulenin, whereas variations of the epoxy-oxo-amide structural element led to a complete loss of inhibition potency. The results indicate that the naturally occurring antibiotic cerulenin is the most active specific inhibitor of de novo fatty-acid biosynthesis, but the formation of the hydroxylactam ring seems to be an essential requirement for the inhibitory activity. Those structural analogues of cerulenin, which can no longer form a hydroxylactam ring, do not possess any inhibitory capacity.

scholarly journals Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar )

Lipids ◽  
2019 ◽  
Vol 54 (11-12) ◽  
pp. 725-739 ◽  
Author(s):  
Nicola A. Irvine ◽  
Bente Ruyter ◽  
Tone‐Kari Østbye ◽  
Anna K. Sonesson ◽  
Karen A. Lillycrop ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Fatty Acid ◽  
Atlantic Salmon ◽  
Fish Oil ◽  
Salmo Salar ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Atlantic Salmon Salmo Salar ◽  
Dietary Fish
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