Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method

Lipids ◽  
1991 ◽  
Vol 26 (10) ◽  
pp. 853-856 ◽  
Author(s):  
Zhen-Yue Jiang ◽  
Alison C. S. Woollard ◽  
Simon P. Wolff
Keyword(s):  
Xylenol Orange ◽  
Lipid Hydroperoxide

Lipid Hydroperoxide Determination in Dark Chicken Meat through a Ferrous Oxidation−Xylenol Orange Method

2000 ◽  
Vol 48 (9) ◽  
pp. 4136-4143 ◽  
Author(s):  
Anna Grau ◽  
Rafael Codony ◽  
Magda Rafecas ◽  
Ana C. Barroeta ◽  
Francesc Guardiola
Keyword(s):  
Xylenol Orange ◽  
Lipid Hydroperoxide ◽  
Chicken Meat ◽  
Ferrous Oxidation

scholarly journals Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Relevance to determination of total plasma lipid hydroperoxide concentrations

1996 ◽  
Vol 313 (3) ◽  
pp. 781-786 ◽  
Author(s):  
Jaffar NOUROOZ-ZADEH ◽  
Jarad TAJADDINI-SARMADI ◽  
K. L. Eddie LING ◽  
Simon P. WOLFF
Keyword(s):  
Total Lipid ◽  
Xylenol Orange ◽  
Low Density Lipoprotein ◽  
Lipid Hydroperoxide ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Ldl Oxidation ◽  
Lipid Hydroperoxides ◽  
Very Low Density Lipoprotein ◽  
Low Density

High-density lipoprotein (HDL) has been proposed as the principal carrier of hydroperoxides in plasma, based upon data gathered with an HPLC-chemiluminescence technique. To test this hypothesis we have measured total lipid hydroperoxides in native plasma using the ferrous oxidation in Xylenol Orange (FOX) assay and then fractionated plasma into very-low-density lipoprotein, low-density lipoprotein (LDL) and HDL fractions. Hydroperoxides were found to accumulate principally (more than 65%) in LDL, as judged by hydroperoxide content per amount of protein or cholesterol, or expressed as a proportion of total hydroperoxide in plasma. Plasma was also incubated at 37 °C in the presence and absence of 2,2´-azo-bis-(2-amidinopropane) hydrochloride (AAPH), an azo-initiator of lipid peroxidation. The majority of hydroperoxides generated in plasma were recovered in the LDL fraction. Furthermore, when isolated lipoproteins were subject to oxidation initiated by AAPH, very-low-density lipoprotein and LDL showed the greatest propensity for hydroperoxide accumulation, whereas HDL seemed relatively resistant. Estimates for plasma and LDL peroxidation based upon techniques which measure total lipid hydroperoxides suggest that levels of hydroperoxides in plasma and LDL are far higher than that those estimates generated by ostensibly more selective techniques. Higher levels of hydroperoxides in LDL than those reported by HPLC-chemiluminescence also seem in greater accordance with other available data concerning LDL oxidation.

scholarly journals Formation of α-tocopherol hydroperoxide and α-tocopheroxyl radical: relevance for photooxidative stress in Arabidopsis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Aditya Kumar ◽  
Ankush Prasad ◽  
Pavel Pospíšil
Keyword(s):  
Singlet Oxygen ◽  
Peroxyl Radical ◽  
Higher Plants ◽  
Xylenol Orange ◽  
Hydrogen Abstraction ◽  
Lipid Hydroperoxide ◽  
Antioxidant Defense System ◽  
High Light ◽  
Oxidation Products ◽  
Tocopheroxyl Radical

AbstractTocopherols, lipid-soluble antioxidants play a crucial role in the antioxidant defense system in higher plants. The antioxidant function of α-tocopherol has been widely studied; however, experimental data on the formation of its oxidation products is missing. In this study, we attempt to provide spectroscopic evidence on the detection of oxidation products of α-tocopherol formed by its interaction with singlet oxygen and lipid peroxyl radical. Singlet oxygen was formed using photosensitizer rose bengal and thylakoid membranes isolated from Arabidopsis thaliana. Singlet oxygen reacts with polyunsaturated fatty acid forming lipid hydroperoxide which is oxidized by ferric iron to lipid peroxyl radical. The addition of singlet oxygen to double bond carbon on the chromanol head of α-tocopherol forms α-tocopherol hydroperoxide detected using fluorescent probe swallow-tailed perylene derivative. The decomposition of α-tocopherol hydroperoxide forms α-tocopherol quinone. The hydrogen abstraction from α-tocopherol by lipid peroxyl radical forms α-tocopheroxyl radical detected by electron paramagnetic resonance. Quantification of lipid and protein hydroperoxide from the wild type and tocopherol deficient (vte1) mutant Arabidopsis leaves using a colorimetric ferrous oxidation-xylenol orange assay reveals that α-tocopherol prevents formation of both lipid and protein hydroperoxides at high light. Identification of oxidation products of α-tocopherol might contribute to a better understanding of the protective role of α-tocopherol in the prevention of oxidative damage in higher plants at high light.

The influence of harmful physical factors and industrial dust on changes in some biochemical and functional indicators of the cardiovascular system and respiratory system in workers engaged in underground mining of ore

2019 ◽  
pp. 920-925
Author(s):  
M. A. Zemlyanova ◽  
Yu. V. Koldibekova ◽  
V. M. Ukhabov
Keyword(s):  
Antioxidant Activity ◽  
Cardiovascular System ◽  
Working Conditions ◽  
Biochemical Parameters ◽  
Fine Particles ◽  
Underground Mining ◽  
Lipid Hydroperoxide ◽  
Physical Factors ◽  
Industrial Dust ◽  
High Degree

Introduction. The health of workers is determined by both social and individual, as well as production factors, including noise, vibration and dust, characteristic of technological processes in mining enterprises. Industrial noise above 90 dBA and dust in the form of suspended and fine particles causes dysfunction of the cardiovascular system and respiratory organs.The aim of the study is to assess the impact of harmful physical factors and industrial dust on changes in some biochemical and functional indicators of the cardiovascular system and respiratory system of employees of the enterprise for underground mining of ore.Materials and methods. The assessment of working conditions of workers engaged in underground mining and processing of chrome ores, the analysis of industrial dust on the dimension and quantitative content of fine particles PM10 and PM2. 5, the study of biochemical parameters of lipid metabolism, oxidant and antioxidant systems, indicators of respiratory function. Results. Harmful working conditions (class 3.1–3.4) in terms of noise and vibration were revealed at the workplaces of employees of the main specialties observation group. In the air of the working zone, the highest content of fine particles PM2.5 (2.68±0.54 mg/m3) and PM10 (4.64±0.93 mg/m3) was established at the site of drilling operations and cleaning of the bottom-hole space. Deviations of biochemical parameters characterizing intensification of free radical processes and antioxidant protection, imbalance of lipid spectrum parameters, and violation of functional parameters of external respiration were revealed. A high degree of connection with the working conditions of the frequency of increased levels of lipid hydroperoxide and antioxidant activity (EF=60.71–65.84%) was established.Conclusions. In high noise level (more than 94 dBA), general and local vibration (more than 116 and 127 dB respectively) and the content of fine particles PM2. 5 (more of 2.14 mg/m3) and PM10 (over of 3.71 mg/m3) at underground mining of chrome ore workers have identified abnormalities in the form of increase 1.6 times the level of lipid hydroperoxides and total antioxidant activity, reducing to 1.2 times the level of high density lipoprotein, improving 1.2–1.3 times of low-density lipoproteins, triglycerides, and atherogenic index in blood serum, peak expiratory flow. A high degree of professional conditioning have the frequency of increasing the level of lipid hydroperoxide in blood serum and antioxidant activity of blood plasma.

New dosimetric approach for multidimensional dose evaluation in gamma knife radiosurgery

2000 ◽  
Vol 93 (supplement_3) ◽  
pp. 239-242 ◽  
Author(s):  
R. Foroni ◽  
G. Gambraini ◽  
U. Danesi ◽  
M. Mauri ◽  
E. Pompilio ◽  
...  
Keyword(s):  
Gamma Knife ◽  
Metal Ion ◽  
Light Transmission ◽  
Xylenol Orange ◽  
Ferrous Sulphate ◽  
Gamma Knife Radiosurgery ◽  
Absorbed Dose ◽  
Content Type ◽  
3D Representation ◽  
Fine Print

✓ During the past two decades, the progress in computerized treatment planning systems has led to more accurate imaging and therapy by using the gamma knife, especially with the smallest collimators (4 mm). However, the ionization chambers that have been used to calibrate the gamma knife are not useful with the smallest collimators because the chambers are too big compared with the irradiated volume. Therefore, it is important to develop more suitable dosimeters. This study proposes a new dosimeter method. The FriXyGel method proposed here is based on a phantom dosimeter, an acquisition chain, and dedicated software. This dosimeter uses an agarose gel into which a ferrous sulphate solution (Fricke solution) and a metal ion indicator (xylenol orange) are incorporated. The absorbed dose is detected through measurements of visible light transmission, imaged by means of a charge-coupled device camera provided with a suitable optical filter. Gel layers are imaged before and after irradiation, and the differences in light absorption are related to the absorbed dose. By choosing convenient thickness of gel layers and by building up a phantom with different gel slices, it is possible to obtain a three-dimensional (3D) representation of the absorbed dose. The final 3D representation is reached after several mathematical processes have been applied to the images. The first step identifies and reduces all factors that could alter the original data, such as nonuniformity in illumination. Then, after calibration procedures, it is possible to obtain absorbed dose values and to discover their 3D representation. This goal has been reached by developing appropriate software that performs all the calculations necessary for spatial representation routines and prompt comparison with theoretical calculations.

Xylenol-orange-assay-of-hydrogen-peroxide for Measuring Uricase Activity and Recognizing High-activity Uricase Mutant

2013 ◽  
Vol 19 (3) ◽  
pp. 523-527 ◽  
Author(s):  
Miaomiao LIU ◽  
Juan FENG ◽  
Hongbo LIU ◽  
Xiaolan YANG ◽  
Liping FENG ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
High Activity ◽  
Xylenol Orange ◽  
Uricase Activity

Assessment of Structural Stability of Pure and Xylenol Orange Dye Doped Potassium Dihydroegn Phosphate Probed by Shock Waves

2021 ◽  
Author(s):  
A. Sivakumar ◽  
A. Saranraj ◽  
S. Sahaya Jude Dhas ◽  
P. Sivaprakash ◽  
S. Arumugam ◽  
...  
Keyword(s):  
Shock Waves ◽  
Structural Stability ◽  
Xylenol Orange

scholarly journals Chronic High Fat Diet Intake Impairs Hepatic Metabolic Parameters in Ovariectomized Sirt3 KO Mice

2021 ◽  
Vol 22 (8) ◽  
pp. 4277
Author(s):  
Marija Pinterić ◽  
Iva I. Podgorski ◽  
Marijana Popović Hadžija ◽  
Ivana Tartaro Bujak ◽  
Ana Tadijan ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Metabolic Diseases ◽  
Body Weight Gain ◽  
Lipid Hydroperoxide ◽  
Metabolic Stress ◽  
Lipid Hydroperoxides ◽  
Ros Scavenging ◽  
High Fat ◽  
Preclinical Research ◽  
Female Mice

High fat diet (HFD) is an important factor in the development of metabolic diseases, with liver as metabolic center being highly exposed to its influence. However, the effect of HFD-induced metabolic stress with respect to ovary hormone depletion and sirtuin 3 (Sirt3) is not clear. Here we investigated the effect of Sirt3 in liver of ovariectomized and sham female mice upon 10 weeks of feeding with standard-fat diet (SFD) or HFD. Liver was examined by Folch, gas chromatography and lipid hydroperoxide analysis, histology and oil red staining, RT-PCR, Western blot, antioxidative enzyme and oxygen consumption analyses. In SFD-fed WT mice, ovariectomy increased Sirt3 and fatty acids synthesis, maintained mitochondrial function, and decreased levels of lipid hydroperoxides. Combination of ovariectomy and Sirt3 depletion reduced pparα, Scd-1 ratio, MUFA proportions, CII-driven respiration, and increased lipid damage. HFD compromised CII-driven respiration and activated peroxisomal ROS scavenging enzyme catalase in sham mice, whereas in combination with ovariectomy and Sirt3 depletion, increased body weight gain, expression of NAFLD- and oxidative stress-inducing genes, and impaired response of antioxidative system. Overall, this study provides evidence that protection against harmful effects of HFD in female mice is attributed to the combined effect of female sex hormones and Sirt3, thus contributing to preclinical research on possible sex-related therapeutic agents for metabolic syndrome and associated diseases.

scholarly journals Glycomacropeptide Ameliorates Indomethacin-Induced Enteropathy in Rats by Modifying Intestinal Inflammation and Oxidative Stress

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2351 ◽  
Author(s):  
Daniel Cervantes-García ◽  
Armida I. Bahena-Delgado ◽  
Mariela Jiménez ◽  
Laura E. Córdova-Dávalos ◽  
Vanessa Ruiz-Esparza Palacios ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Intestinal Inflammation ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Lipid Hydroperoxide ◽  
Neutrophil Infiltration ◽  
Clinical Problem ◽  
Intestinal Damage ◽  
Nsaid Enteropathy ◽  
Anti Inflammatory

Nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is considered a serious and increasing clinical problem without available treatment. Glycomacropeptide (GMP) is a 64-amino acid peptide derived from milk κ-casein with numerous biological activities. The aim of this study was to investigate the protective effect of GMP on NSAID enteropathy in rats. Enteropathy was induced by seven days oral indomethacin administration. Rats were orally GMP treated from seven days previous and during the establishment of the enteropathy model. Changes in metabolism, hematological and biochemical blood alterations, intestinal inflammation and oxidative damage were analyzed. Integrity barrier markers, macroscopic intestinal damage and survival rate were also evaluated. GMP treatment prevented anorexia and weight loss in animals. Furthermore, prophylaxis with GMP ameliorated the decline in hemoglobin, hematocrit, albumin and total protein levels. The treatment had no therapeutic efficacy on the decrease of occludin and mucin (MUC)-2 expression in intestinal tissue. However, GMP markedly decreased neutrophil infiltration, and CXCL1, interleukin-1β and inducible nitric oxide synthase expression. Nitric oxide production and lipid hydroperoxide level in the small intestine were also diminished. These beneficial effects were mirrored by preventing ulcer development and increasing animal survival. These results suggest that GMP may protect against NSAID enteropathy through anti-inflammatory and antioxidant properties.

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