A novel spectrophotometric assay for lipase activity utilizingcis-parinaric acid

Lipids ◽  
1989 ◽  
Vol 24 (6) ◽  
pp. 518-525 ◽  
Author(s):  
A. M. Rogel ◽  
W. L. Stone ◽  
F. O. Adebonojo
Keyword(s):  
Lipase Activity ◽  
Spectrophotometric Assay ◽  
Parinaric Acid

Assaying Arabidopsis lipase activity

2000 ◽  
Vol 28 (6) ◽  
pp. 773-775 ◽  
Author(s):  
F. Beisson ◽  
V. Arondel ◽  
R. Verger
Keyword(s):  
Lipase Activity ◽  
Crude Extract ◽  
Specific Activity ◽  
Colorimetric Assay ◽  
Fluorescence Assay ◽  
Plant Lipase ◽  
Parinaric Acid ◽  
Fluorescence Assays ◽  
Continuous Assay

A low lipase activity from a crude extract of Arabidopsis seedlings was assayed using three sensitive methods (radiolabelled triacylglycerols, commercial resorufin ester and triacylglycerols containing the naturally fluorescent parinaric acid as substrates). The specific activity of the extract was found to be similar using the three methods. However, the plant lipase activity measured using the radioactivity and the fluorescence assays could be abolished by heating the extract, contrary to the apparent activity measured using the commercial colorimetric assay. Unlike the radioactivity assay, the fluorescence assay can be monitored continuously. The parinaric acid-based method is therefore the only one to provide a sensitive, specific and continuous assay.

An Ultraviolet Spectrophotometric Assay for Measuring Lipase Activity Using Long-Chain Triacyglycerols from Aleurites fordii Seeds

2002 ◽  
Vol 303 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Gaëlle Pencreac'h ◽  
Jean Graille ◽  
Michel Pina ◽  
Robert Verger
Keyword(s):  
Lipase Activity ◽  
Spectrophotometric Assay ◽  
Long Chain
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