Pulmonary surfactant lipid synthesis from ketone bodies, lactate and glucose in newborn rats

Lipids ◽  
1985 ◽  
Vol 20 (12) ◽  
pp. 835-841 ◽  
Author(s):  
Patricia M. Sheehan ◽  
Yu-Yan Yeh
Keyword(s):  
Pulmonary Surfactant ◽  
Ketone Bodies ◽  
Lipid Synthesis ◽  
Newborn Rats ◽  
Surfactant Lipid

IL-4 increases surfactant and regulates metabolism in vivo

2000 ◽  
Vol 278 (1) ◽  
pp. L75-L80 ◽  
Author(s):  
Machiko Ikegami ◽  
Jeffrey A. Whitsett ◽  
Zissis C. Chroneos ◽  
Gary F. Ross ◽  
Jacquelyn A. Reed ◽  
...  
Keyword(s):  
Lipid Synthesis ◽  
Fold Increase ◽  
Surfactant Protein ◽  
Clara Cells ◽  
Wild Type ◽  
Surfactant Lipid ◽  
Alveolar Proteinosis ◽  
Selective Increase

Mice that express interleukin (IL)-4 in Clara cells (CCSP-IL-4) develop chronic airway inflammation and an alveolar proteinosis-like syndrome. To identify the role of IL-4 in surfactant homeostasis, we measured lipid and protein metabolism in the lungs of CCSP-IL-4 mice in vivo. Alveolar saturated phosphatidylcholine (Sat PC) pools were increased 6.5-fold and lung tissue Sat PC pools were increased 4.8-fold in the IL-4 transgenic mice. Whereas surfactant protein (SP) A was increased proportionately to Sat PC, SP-D was increased approximately 90-fold in the IL-4 mice compared with wild-type mice and was associated with 2.8-fold increase in SP-D mRNA. The incorporation of palmitate and choline into Sat PC was increased about twofold in CCSP-IL-4 mice. Although trace doses of radiolabeled Sat PC were cleared from the air spaces and lungs of CCSP-IL-4 mice more slowly than in wild-type mice, net clearance of Sat PC from the lungs of CCSP-IL-4 mice was sixfold higher in the IL-4 mice than in wild-type mice because of the larger Sat PC pool sizes. Expression of IL-4 in Clara cells increased surfactant lipid synthesis and clearance, establishing a new equilibrium with increased surfactant pools and an alveolar proteinosis associated with a selective increase in SP-D protein, demonstrating a previously unexpected effect of IL-4 in pulmonary surfactant homeostasis.

Pulmonary Surfactant Migrates into the Alveolar Capillaries of Newborn Rats: An Immunoelectron Microscopic Study

1996 ◽  
Vol 156 (1) ◽  
pp. 11-21 ◽  
Author(s):  
M. Wasowicz ◽  
K. Kashima ◽  
S. Yokoyama ◽  
I. Nakayama
Keyword(s):  
Microscopic Study ◽  
Pulmonary Surfactant ◽  
Newborn Rats

GLUCAGON RELEASE FROM THE PANCREAS OF THE NEWBORN RAT

1972 ◽  
Vol 54 (3) ◽  
pp. 493-504 ◽  
Author(s):  
J. C. EDWARDS ◽  
K. ASPLUND ◽  
G. LUNDQVIST
Keyword(s):  
Inhibitory Action ◽  
Octanoic Acid ◽  
Ketone Bodies ◽  
Solid Phase ◽  
Glucagon Release ◽  
Newborn Rats ◽  
Newborn Rat ◽  
Stimulatory Action ◽  
Rat Pancreas ◽  
Solid Phase Radioimmunoassay

SUMMARY A solid phase radioimmunoassay for glucagon was specially modified in order to overcome the problems involved in the measurement of glucagon release from incubated pieces of pancreas. The modified immunoassay procedure was used to study glucagon release from pieces of pancreas taken from newborn rats aged from 1 to 20 days. The glucagon content of rat pancreas was also measured during this period. It was found that glucagon release from rat pancreas was stimulated by arginine and inhibited by octanoic acid at 1 and 2 days of age. However, glucagon release at 3 days of age was low, and between 3 and 7 days of age glucagon release could not be inhibited by octanoic acid or stimulated by arginine. At 10 and 20 days of age, the stimulatory action of arginine and the inhibitory action of octanoic acid were again noted. Glucagon release, measured at several ages, was not significantly affected by changes in glucose concentration. The glucagon content of the rat pancreas rose to a maximum at 5 days of age and then decreased gradually over a period of 90 days. It is suggested that the low rate of glucagon release between 3 and 7 days of age may be a result of the high levels of blood fatty acids and ketone bodies found in the rat during this period.

scholarly journals Anionic pulmonary surfactant lipid regulation of innate immunity

2012 ◽  
Vol 6 (3) ◽  
pp. 243-246 ◽  
Author(s):  
Mari Numata ◽  
Pitchaimani Kandasamy ◽  
Dennis R Voelker
Keyword(s):  
Innate Immunity ◽  
Pulmonary Surfactant ◽  
Surfactant Lipid ◽  
Lipid Regulation

scholarly journals Apolipoprotein A-I, A-II, C-II, and H expression in the developing lung and sex difference in surfactant lipids

2008 ◽  
Vol 200 (3) ◽  
pp. 321-330 ◽  
Author(s):  
Pierre R Provost ◽  
Eric Boucher ◽  
Yves Tremblay
Keyword(s):  
Sex Difference ◽  
Expression Profiles ◽  
Secretory Granules ◽  
Lipid Synthesis ◽  
Fetal Lung ◽  
Basal Membrane ◽  
Mrna Accumulation ◽  
Apolipoprotein A ◽  
Surfactant Lipid

A sex difference in surfactant lipids is associated with a higher incidence of respiratory distress syndrome for males in cases of preterm birth. In animal models, the sex difference in surfactant lipids was shown to be androgen receptor-dependent. This report examines expression of apolipoprotein (apo)A-I, apoA-II, apoC-II, apoE, apoH, and lipoprotein lipase (LPL) by quantitative real-time PCR in pools of male and female fetal lung tissues from various mouse litters from gestation day (GD) 15.5 to 18.5, and in various adult tissues. Although the expression profiles of ApoA-I, ApoA-II, ApoC-II, and ApoH are complex, these genes are co-regulated and they all present a sex difference (P=0.0896, 0.0896, 0.0195, and 0.0607 respectively) with higher expression for females for several litters. Pulmonary expression of apoA-I, apoA-II, and apoH were specific to the developing lung. ApoE and LPL mRNAs showed a significant increase from GD 17.5 to 18.5. An increase in apoA-I-, apoA-II-, apoC-II-, and apoH-mRNA accumulation was observed from GD 16.5 to 17.5 in correlation with the emergence of mature type II pneumonocytes. These four apolipoprotein genes are co-regulated with type 2 and 5 17β-hydroxysteroid dehydrogenases, which are respectively involved in inactivation and synthesis of androgens. Finally, apoC-II was detected by immunohistochemistry in epithelial cells of the distal epithelium. Positive signals looking like secretory granules were located near the basal membrane. Our results are compatible with a role for apolipoproteins in lipid metabolism and transport in the developing lung in association with the sex difference in surfactant lipid synthesis.

scholarly journals Pulmonary Surfactant Lipid Reorganization Induced by the Adsorption of the Oligomeric Surfactant Protein B Complex

2020 ◽  
Vol 432 (10) ◽  
pp. 3251-3268 ◽  
Author(s):  
Juho Liekkinen ◽  
Giray Enkavi ◽  
Matti Javanainen ◽  
Barbara Olmeda ◽  
Jesús Pérez-Gil ◽  
...  
Keyword(s):  
Pulmonary Surfactant ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Surfactant Lipid ◽  
Protein B
Sign in / Sign up

Export Citation Format

Share Document