Effects of purified eicosapentaenoic acid on arachidonic acid metabolism in cultured murine aortic smooth muscle cells, vessel walls and platelets

Lipids ◽  
1983 ◽  
Vol 18 (1) ◽  
pp. 42-49 ◽  
Author(s):  
I. Morita ◽  
Y. Saito ◽  
W. C. Chang ◽  
S. Murota
Keyword(s):  
Arachidonic Acid ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Eicosapentaenoic Acid ◽  
Acid Metabolism ◽  
Muscle Cells ◽  
Arachidonic Acid Metabolism ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Vessel Walls

Mechanism of angiotensin II-induced arachidonic acid metabolite release in aortic smooth muscle cells: involvement of phospholipase D

1997 ◽  
Vol 136 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Junji Shinoda ◽  
Osamu Kozawa ◽  
Atsushi Suzuki ◽  
Yasuko Watanabe-Tomita ◽  
Yutaka Oiso ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cells ◽  
Phospholipase D ◽  
Muscle Cells ◽  
Dependent Manner ◽  
Ang Ii ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Abstract In a previous study, we have shown that angiotensin II (Ang II) activates phosphatidylcholinehydrolyzing phospholipase D due to Ang II-induced Ca2+ influx from extracellular space in subcultured rat aortic smooth muscle cells. In the present study, we have investigated the role of phospholipase D in Ang II-induced arachidonic acid (AA) metabolite release and prostacyclin synthesis in subcultured rat aortic smooth muscle cells. Ang II significantly stimulated AA metabolite release in a concentration-dependent manner in the range between 1 nmol/l and 0·1 μmol/l. d,l-Propranolol hydrochloride (propranolol), an inhibitor of phosphatidic acid phosphohydrolase, significantly inhibited the Ang II-induced release of AA metabolites. The Ang II-induced AA metabolite release was reduced by chelating extracellular Ca2+ with EGTA. Genistein, an inhibitor of protein tyrosine kinases, significantly suppressed the Ang II-induced AA metabolite release. 1,6-Bis-(cyclohexyloximinocarbonylamino)-hexane (RHC-80267), a potent and selective inhibitor of diacylglycerol lipase, significantly inhibited the Ang II-induced AA metabolite release. Both propranolol and RHC-80267 inhibited the Ang II-induced synthesis of 6-keto-prostaglandin F1α, a stable metabolite of prostacyclin. The synthesis was suppressed by genistein. These results strongly suggest that the AA metabolite release induced by Ang II is mediated, at least in part, through phosphatidylcholine hydrolysis by phospholipase D activation in aortic smooth muscle cells. European Journal of Endocrinology 136 207–212

Eicosapentaenoic acid enhances myo-inositol uptake in cultured human aortic smooth muscle cells

Life Sciences ◽  
1994 ◽  
Vol 55 (1) ◽  
pp. PL15-PL18 ◽  
Author(s):  
Yukichi Okuda ◽  
Motomi Ezure ◽  
Toshie Sawada ◽  
Masakazu Mizutani ◽  
Kenichiro Tsukahara ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Eicosapentaenoic Acid ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Inositol Uptake

scholarly journals CYP26B1 Plays a Major Role in the Regulation of All-trans-Retinoic Acid Metabolism and Signaling in Human Aortic Smooth Muscle Cells

2011 ◽  
Vol 48 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Pauline Ajok Ocaya ◽  
Ali Ateia Elmabsout ◽  
Peder Stefan Olofsson ◽  
Hans Törmä ◽  
Andreas Carl Gidlöf ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Retinoic Acid ◽  
Smooth Muscle Cells ◽  
Acid Metabolism ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle
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