Rapid determination of lipids containing free amino groups with trinitrobenzene sulfonic acid reagent

Lipids ◽  
1967 ◽  
Vol 2 (1) ◽  
pp. 87-88 ◽  
Author(s):  
A. N. Siakotos
Keyword(s):  
Free Amino ◽  
Sulfonic Acid ◽  
Rapid Determination ◽  
Trinitrobenzene Sulfonic Acid ◽  
Amino Groups ◽  
Acid Reagent

Comparison of the Hide Powder Azure and Casein-Trinitrobenzene Sulfonic Acid Methods for Determining Proteolysis in Skim Milk

1984 ◽  
Vol 47 (6) ◽  
pp. 476-480 ◽  
Author(s):  
ROBIN C. McKELLAR
Keyword(s):  
Free Amino ◽  
Sulfonic Acid ◽  
Degradation Products ◽  
Skim Milk ◽  
Sodium Caseinate ◽  
Buffer System ◽  
Trinitrobenzene Sulfonic Acid ◽  
Insoluble Protein ◽  
Slope Ratio ◽  
Amino Groups

Three extracellular psychrotroph proteinases were assayed in a defined buffer system and in skim milk, using the insoluble protein-dye complex, hide powder azure (HPA), as well as by quantifying free amino groups released from casein with trinitrobenzene-sulfonic acid (TNBS). Apparent Km values for HPA of approximately 60 mg/ml were found for each enzyme while for sodium caseinate, the apparent Km of 9.14 mg/ml for enzyme ATCC 15456 was 3-fold greater than for enzymes 13 and 32A. Vmax values for enzyme ATCC 15456 were 3 to 3.5 and 1.6 to 2.8-fold lower than for the other two enzymes with HPA and sodium caseinate, respectively. When activities in skim milk were being determined, trichloroacetic acid (TCA) concentrations of 2 and 8% for HPA and TNBS, respectively, were chosen to maximize recovery of degradation products and minimize background. Proteolysis measurements in skim milk with the two methods were closely correlated (r = 0.903 to 0.920) for each of the three enzymes. Detectability values (obtained by dividing the standard error of estimate for each method by its slope ratio) of 0.098 and 0.093 for the HPA and TNBS methods, respectively, indicate that the two methods do not differ significantly (P>0.05) in reliability.

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

scholarly journals Enhancement of 5HT Uptake by Picryl Sulfonic Acid Amino Group Modification in the Platelet Membrane

1977 ◽  
Author(s):  
C.B. Harbury ◽  
M.E. Janszen ◽  
S. Rutherford
Keyword(s):  
Free Amino ◽  
Sulfonic Acid ◽  
T Test ◽  
Platelet Membrane ◽  
Amino Groups ◽  
Functional Importance ◽  
Clot Retraction ◽  
Release Reaction ◽  
Group Modification ◽  
Paired T Test

The main purpose of this experiment was to assess the functional importance of platelet membrane amino groups (NH2). Picryl Sulfonic Acid (PSA) is a known NH2 modifier. Platelets were harvested, exposed to 0.25 - 32 mM PSA for 15 minutes at 22°C. They were then washed and functionally tested. The release was measured by C5HT release. The release reaction was induced by 0.1, 1, and 10 u/ml Thrombin (THR), Trypsin (TRY), Collagen (COL), and Latex particles (LAT). There was gradual but never complete inhibition of the release reaction. At 16 mM PSA, the platelets started to lose small amounts of 5HT (4%). Clot retraction was not affectedby 1 to 32 mil PSA. 5HT uptake was enhanced at 0.1 to 4 mM PSA (paired t test, p<0.01) and maximal at 2 mM PSA.Pre-incubation with 0.25 and 0.5 mM PSA in the presence of calcium enhanced the PSA effect on rate of 5HT uptake (paired t test, p<0.01). This may be due to the enhanced availability of amino groups to PSA in the presence of Ca, speculated by Godin to be due to a lipid effect (1972). Incorporation of PSA into the membrane was increased in the presence of Ca (O.D. at 335 nm). In summary, PSA modification of platelet membrane NH2 groups partially but never completely interferes with the induction of the release reaction. The inhibition was not specific with respect to the release inducing agent. Clot retraction remains intact. The observed significant enhancement in rate of 5HT uptake may be specific or may be due to neutralization of the charge on free amino groups, which repels 5HT.

scholarly journals Spectrophotometric Assay of some Nitrogen Containing Drugs in Pharmaceutical Formulations using p-Chloranilic Acid Reagent

2013 ◽  
Vol 9 (1) ◽  
pp. 1798-1809 ◽  
Author(s):  
Theia’a N. Al-Sabha ◽  
Mahmood A. Hasan ◽  
Huda A. Ibrahim
Keyword(s):  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Charge Transfer Complexes ◽  
Amino Groups ◽  
Chloranilic Acid ◽  
Terbutaline Sulfate ◽  
Acid Reagent ◽  
Sulfacetamide Sodium ◽  
Better Than

A spectrophotometric method is developed for the determination of some drugs containing amino groups (sulfacetamide sodium, lidocaine and terbutaline sulfate) based on their reaction with p-chloranilic acid reagent in an organic medium forming colored charge transfer complexes. The complexes have maximum absorptions at 530 and 527 nm for sulfacetamide sodium and lidocaine respectively, but terbutaline sulfate gave two maximum absorptions at 529 and 319 nm. Beers law is obeyed over the concentration range of 10-60 µg.ml-1 for sulfacetamide sodium and lidocaine and 5-70 µg.ml-1 for terbutaline sulfate. The molar absorptivity values are 0.940×103, 0.913×103 L.mol-1.cm-1 for sulfacetamide sodium and lidocaine respectively while terbutaline sulfate gave 0.987×103 L.mol-1.cm-1 at 529 nm and 7.407×103 L.mol-1.cm-1 at 319 nm with accuracy range between 100.20% and 101.42% and RSD better than 3.15% for all drugs. The method is applied successfully for determination of these drugs in pharmaceutical formulations and compared favorably with British Pharmacopeia standard methods. F and t tests are less than the tabulated values at 95% confidence level.

A simple colorimetric determination of the free amino groups in water soluble chitin derivatives using genipin

2010 ◽  
Vol 19 (3) ◽  
pp. 683-689 ◽  
Author(s):  
Luz M. Ramos-Ponce ◽  
Mireille Vega ◽  
Georgina C. Sandoval-Fabián ◽  
Edith Colunga-Urbina ◽  
Nagamani Balagurusamy ◽  
...  
Keyword(s):  
Free Amino ◽  
Water Soluble ◽  
Colorimetric Determination ◽  
Amino Groups ◽  
Chitin Derivatives
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