Immunolocalizations of human gelatinase (type IV collagenase, MMP-9) and TIMP (tissue inhibitor of metalloproteinases) in normal epidermis and some epidermal tumors

1996 ◽  
Vol 288 (5-6) ◽  
pp. 239-244 ◽  
Author(s):  
T. Kobayashi ◽  
N. Onoda ◽  
T. Takagi ◽  
H. Hori ◽  
S. Hattori ◽  
...  
Keyword(s):  
Tissue Inhibitor Of Metalloproteinases ◽  
Tissue Inhibitor ◽  
Type Iv Collagenase ◽  
Type Iv ◽  
Normal Epidermis

Stability analysis of latent and active 72-kDa type IV collagenase: The role of tissue inhibitor of metalloproteinases-2 (TIMP-2)

Biochemistry ◽  
1993 ◽  
Vol 32 (6) ◽  
pp. 1583-1592 ◽  
Author(s):  
David E. Kleiner ◽  
Ari Tuuttila ◽  
Karl Tryggvason ◽  
William G. Stetler-Stevenson
Keyword(s):  
Stability Analysis ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Tissue Inhibitor ◽  
Type Iv Collagenase ◽  
Type Iv

Higher-order complex formation between the 72-kilodalton type IV collagenase and tissue inhibitor of metalloproteinases-2

Biochemistry ◽  
1992 ◽  
Vol 31 (6) ◽  
pp. 1665-1672 ◽  
Author(s):  
David E. Kleiner ◽  
Edward J. Unsworth ◽  
Henry C. Krutzsch ◽  
William G. Stetler-Stevenson
Keyword(s):  
Complex Formation ◽  
Higher Order ◽  
Order Complex ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Tissue Inhibitor ◽  
Type Iv Collagenase ◽  
Type Iv

Gene Expression of Metalloproteinases and Their Inhibitor in Renal Tissue of New Zealand Black/White F1 Mice

1993 ◽  
Vol 85 (3) ◽  
pp. 295-301 ◽  
Author(s):  
Tsukasa Nakamura ◽  
Isao Ebihara ◽  
Shiori Osada ◽  
Toshimasa Takahashi ◽  
Masatoshi Yamamoto ◽  
...  
Keyword(s):  
New Zealand ◽  
Mononuclear Cells ◽  
Experimental Period ◽  
Renal Tissue ◽  
Mrna Levels ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Tissue Inhibitor ◽  
Type Iv Collagenase ◽  
Type Iv ◽  
Extracellular Matrix Components

1. The present study was carried out to determine how levels of the mRNA of metalloproteinases (metalloproteinase-1, 72 kDa type IV collagenase, metalloproteinase-3 and 92 kDa type IV collagenase) and tissue inhibitor of metalloproteinases are regulated in the renal tissues of New Zealand Black/White F1 mice. 2. mRNA levels for metalloproteinase-1, 72 kDa type IV collagenase, metalloproteinase-3 and tissue inhibitor of metalloproteinases increased significantly with the progression of nephritis in New Zealand Black/White F1 mice. 3. At 48 weeks of age, the levels of mRNA for metalloproteinase-1, 72 kDa type IV collagenase, metalloproteinase-3 and tissue inhibitor of metalloproteinases increased by 8-, 4-, 8- and 15-fold, respectively, in the renal tissues of New Zealand Black/White F1 mice compared with New Zealand White mice. 4. In the kidneys of New Zealand White mice, however, the mRNA levels for these proteins changed little throughout the experimental period. 5. We could not detect expression of mRNA for 9 2 kDa type IV collagenase in the renal tissue of New Zealand Black/White F1 mice at 8 weeks of age or in New Zealand White mice at 8, 24 or 48 weeks of age, whereas we could detect expression of mRNA for this protein in New Zealand Black/White F1 mice at 24 and 48 weeks of age when mononuclear cells had infiltrated the interstitium and surrounding blood vessels. 6. At 24 weeks of age, New Zealand Black/White F1 mice were divided into two groups and received either methylprednisolone or saline injection for 24 weeks. 7. The development of histopathological lesions and increases in mRNA for metalloproteinases and tissue inhibitor of metalloproteinases were suppressed by treatment with methylprednisolone. 8. These data suggest that abnormal regulation of the genes for metalloproteinases and tissue inhibitor of metalloproteinases contribute to the accumulation of extracellular matrix components in lupus nephritis and that the beneficial effect of methylprednisolone is associated with its ability to suppress the expression of mRNA for metalloproteinases and tissue inhibitor of metalloproteinases.

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