An essential role for DsbA in cytochromec synthesis and formate-dependent nitrite reduction byEscherichia coli K-12

1995 ◽  
Vol 164 (4) ◽  
pp. 301-307 ◽  
Author(s):  
Rachael Metheringham ◽  
Lesley Griffiths ◽  
Helen Crooke ◽  
Stephen Forsythe ◽  
Jeff Cole
Keyword(s):  
Essential Role ◽  
Nitrite Reduction ◽  
K 12 ◽  
Byescherichia Coli

An essential role for DsbA in cytochrome c synthesis and formate-dependent nitrite reduction by Escherichia coli K-12

1995 ◽  
Vol 164 (4) ◽  
pp. 301-307 ◽  
Author(s):  
Rachael Metheringham ◽  
Lesley Griffiths ◽  
Helen Crooke ◽  
S. Forsythe ◽  
J. Cole
Keyword(s):  
Escherichia Coli ◽  
Cytochrome C ◽  
Essential Role ◽  
Nitrite Reduction ◽  
K 12

scholarly journals Interaction between complement subcomponent C1q and bacterial lipopolysaccharides

1989 ◽  
Vol 257 (3) ◽  
pp. 865-873 ◽  
Author(s):  
A Zohair ◽  
S Chesne ◽  
R H Wade ◽  
M G Colomb
Keyword(s):  
Escherichia Coli ◽  
Chemical Modification ◽  
Essential Role ◽  
Wild Strain ◽  
E Coli ◽  
Diethyl Pyrocarbonate ◽  
Direct Binding ◽  
Bacterial Lipopolysaccharides ◽  
K 12

The heptose-less mutant of Escherichia coli, D31m4, bound complement subcomponent C1q and its collagen-like fragments (C1qCLF) with Ka values of 1.4 x 10(8) and 2.0 x 10(8) M-1 respectively. This binding was suppressed by chemical modification of C1q and C1qCLF using diethyl pyrocarbonate (DEPC). To investigate the role of lipopolysaccharides (LPS) in this binding, biosynthetically labelled [14C]LPS were purified from E. coli D31m4 and incorporated into liposomes prepared from phosphatidylcholine (PC) and phosphatidylethanolamine (PE) [PC/PE/LPS, 2:2:1, by wt.]. Binding of C1q or its collagen-like fragments to the liposomes was estimated via a flotation test. These liposomes bound C1q and C1qCLF with Ka values of 8.0 x 10(7) and 2.0 x 10(7) M-1; this binding was totally inhibited after chemical modification of C1q and C1qCLF by DEPC. Liposomes containing LPS purified from the wild-strain E. coli K-12 S also bound C1q and C1qCLF, whereas direct binding of C1q or C1qCLF to the bacteria was negligible. Diamines at concentrations which dissociate C1 into C1q and (C1r, C1s)2, strongly inhibited the interaction of C1q or C1qCLF with LPS. Removal of 3-deoxy-D-manno-octulosonic acid (2-keto-3-deoxyoctonic acid; KDO) from E. coli D31m4 LPS decreases the binding of C1qCLF to the bacteria by 65%. When this purified and modified LPS was incorporated into liposomes, the C1qCLF binding was completely abolished. These results show: (i) the essential role of the collagen-like moiety and probably its histidine residues in the interaction between C1q and the mutant D31m4; (ii) the contribution of LPS, particularly the anionic charges of KDO, to this interaction.

Metabolism of 12-ketooctadecanoic acid byEscherichia coli K-12 andCandida tropicalis

Lipids ◽  
1972 ◽  
Vol 7 (3) ◽  
pp. 178-181
Author(s):  
Michinao Mizugaki ◽  
Masao Sato ◽  
Mitsuru Uchiyama
Keyword(s):  
K 12 ◽  
Byescherichia Coli

scholarly journals A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation

2011 ◽  
Vol 39 (6) ◽  
pp. 1844-1848 ◽  
Author(s):  
Basilio Zafrilla ◽  
Rosa María Martínez-Espinosa ◽  
María José Bonete ◽  
Julea N. Butt ◽  
David J. Richardson ◽  
...  
Keyword(s):  
Essential Role ◽  
Inorganic Nitrogen ◽  
Nitrate Assimilation ◽  
Spectroscopic Characterization ◽  
Redox Properties ◽  
Nitrite Reduction ◽  
Plant Type ◽  
Uv Visible ◽  
Nitrate And Nitrite

In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3−/NO2− reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in the electron transfer cascade required for assimilatory nitrate and nitrite reduction by the cytoplasmic NarB- and NirA-type reductases respectively. UV–visible absorbance and EPR spectroscopic characterization of purified Hv-Fd demonstrate that this protein binds a single [2Fe–2S] cluster, and potentiometric titration reveals that the cluster shares similar redox properties with those present in plant-type ferredoxins.

scholarly journals A reassessment of the range ofc-type cytochromes synthesized byEscherichia coli K-12

1994 ◽  
Vol 119 (1-2) ◽  
pp. 89-94 ◽  
Author(s):  
C. Iobbi-Nivol ◽  
H. Crooke ◽  
L. Griffiths ◽  
J. Grove ◽  
H. Hussain ◽  
...  
Keyword(s):  
K 12 ◽  
Byescherichia Coli

Faint Hα Emission in the Corona and Its Relation to the Prominences

1994 ◽  
Vol 144 ◽  
pp. 339-342
Author(s):  
V. N. Dermendjiev ◽  
Z. Mouradian ◽  
J.- L. Leroy ◽  
P. Duchlev
Keyword(s):  
Magnetic Field ◽  
Solar Corona ◽  
Essential Role ◽  
Hα Emission

AbstractThe relation between episodically observed in the solar corona faint Hαemission structures and the long lived prominences was studied. Particular consideration was given for cases in which the corresponding prominences had undergone DB process. An MHD interpretation of the phenomenon “emissions froides” (cool emission) is proposed in which an essential role plays the prominence supporting magnetic field.

Sign in / Sign up

Export Citation Format

Share Document