Study of the Δ12-desaturase system ofLipomyces starkeyi

Lipids ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 253-259 ◽  
Author(s):  
Anne Lomascolo ◽  
Eric Dubreucq ◽  
Pierre Galzy
Keyword(s):  
Δ12 Desaturase ◽  
Desaturase System

scholarly journals A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66

2021 ◽  
Author(s):  
E-Ming Rau ◽  
Inga Marie Aasen ◽  
Helga Ertesvåg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Palmitoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Gene Annotation ◽  
Vaccenic Acid ◽  
Strain Engineering ◽  
Δ9 Desaturase ◽  
Δ12 Desaturase

Abstract Thraustochytrids are oleaginous marine eukaryotic microbes currently used to produce the essential omega-3 fatty acid docosahexaenoic acid (DHA, C22:6 n-3). To improve the production of this essential fatty acid by strain engineering, it is important to deeply understand how thraustochytrids synthesize fatty acids. While DHA is synthesized by a dedicated enzyme complex, other fatty acids are probably synthesized by the fatty acid synthase, followed by desaturases and elongases. Which unsaturated fatty acids are produced differs between different thraustochytrid genera and species; for example, Aurantiochytrium sp. T66, but not Aurantiochytrium limacinum SR21, synthesizes palmitoleic acid (C16:1 n-7) and vaccenic acid (C18:1 n-7). How strain T66 can produce these fatty acids has not been known, because BLAST analyses suggest that strain T66 does not encode any Δ9-desaturase-like enzyme. However, it does encode one Δ12-desaturase-like enzyme. In this study, the latter enzyme was expressed in A. limacinum SR21, and both C16:1 n-7 and C18:1 n-7 could be detected in the transgenic cells. Our results show that this desaturase, annotated T66Des9, is a Δ9-desaturase accepting C16:0 as a substrate. Phylogenetic studies indicate that the corresponding gene probably has evolved from a Δ12-desaturase-encoding gene. This possibility has not been reported earlier and is important to consider when one tries to deduce the potential a given organism has for producing unsaturated fatty acids based on its genome sequence alone. Key points • In thraustochytrids, automatic gene annotation does not always explain the fatty acids produced. • T66Des9 is shown to synthesize palmitoleic acid (C16:1 n-7). • T66des9 has probably evolved from Δ12-desaturase-encoding genes.

scholarly journals Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

2016 ◽  
Vol 89 ◽  
pp. 405-415 ◽  
Author(s):  
Manuel Fernando Rodríguez-Rodríguez ◽  
Joaquín J. Salas ◽  
Mónica Venegas-Calerón ◽  
Rafael Garcés ◽  
Enrique Martínez-Force
Keyword(s):  
Molecular Cloning ◽  
Camelina Sativa ◽  
Genes Encoding ◽  
Δ12 Desaturase

Expression of Acyl‐lipid Δ12‐desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato

2010 ◽  
Vol 52 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Reza Maali Amiri ◽  
Natalia O. Yur’eva ◽  
Khristina R. Shimshilashvili ◽  
Irina V. Goldenkova‐Pavlova ◽  
Vasiliy P. Pchelkin ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Eukaryotic Cells ◽  
Desaturase Gene ◽  
Acyl Lipid ◽  
Δ12 Desaturase

Analysis of the stearoyl-CoA desaturase system in the Morris hepatoma 7288C and 7288CTC

Lipids ◽  
1984 ◽  
Vol 19 (7) ◽  
pp. 488-491 ◽  
Author(s):  
Raphael A. Zoeller ◽  
Randall Wood
Keyword(s):  
Morris Hepatoma ◽  
Stearoyl Coa Desaturase ◽  
Desaturase System

Studies of the Δ12 desaturase of Carthamus tinctorius L

1985 ◽  
Vol 239 (2) ◽  
pp. 444-454 ◽  
Author(s):  
Joseph M. Gennity ◽  
Paul K. Stumpf
Keyword(s):  
Carthamus Tinctorius ◽  
Δ12 Desaturase

scholarly journals Characterization of the Aspergillus parasiticus Δ12-desaturase gene: a role for lipid metabolism in the Aspergillus–seed interaction

Microbiology ◽  
2004 ◽  
Vol 150 (9) ◽  
pp. 2881-2888 ◽  
Author(s):  
Richard A. Wilson ◽  
Ana M. Calvo ◽  
Perng-Kuang Chang ◽  
Nancy P. Keller
Keyword(s):  
Lipid Metabolism ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Biosynthesis ◽  
Aspergillus Parasiticus ◽  
Oilseed Crop ◽  
Wild Type ◽  
Corn Seed ◽  
Δ12 Desaturase ◽  
Sclerotial Development

In the mycotoxigenic oilseed pathogens Aspergillus flavus and Aspergillus parasiticus and the model filamentous fungus Aspergillus nidulans, unsaturated fatty acids and their derivatives act as important developmental signals that affect asexual conidiospore, sexual ascospore and/or sclerotial development. To dissect the relationship between lipid metabolism and fungal development, an A. parasiticus Δ12-desaturase mutant that was unable to convert oleic acid to linoleic acid and was thus impaired in polyunsaturated fatty acid biosynthesis was generated. The Δ12-desaturase mutant demonstrates delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared to the wild-type. Host colonization is impaired, as reflected by a decrease in conidial production on live peanut and corn seed by the mutant compared to the wild-type. Similarly, the previously isolated A. nidulans Δ12-desaturase mutant has reduced colonization capabilities compared to the wild-type. Therefore, desaturation mutants display a key requisite that affords a genetic solution to oilseed crop contamination by mycotoxigenic Aspergillus species: a reduction in the production of conidia, the infectious particle of the pathogenic aspergilli.

Overexpression of the acyl-lipid Δ12-desaturase gene protects potato plants from low temperature damage

2011 ◽  
Vol 59 (2) ◽  
pp. 103-115 ◽  
Author(s):  
I. Demin ◽  
C. Shimshilashvili ◽  
N. Yur’eva ◽  
N. Naraykina ◽  
I. Goldenkova-Pavlova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Low Temperature ◽  
Cold Stress ◽  
Malonic Dialdehyde ◽  
Plant Leaves ◽  
Desaturase Gene ◽  
Potato Plants ◽  
Acyl Lipid ◽  
Temperature Damage ◽  
Δ12 Desaturase

The responses of plant leaves to chilling were studied in potato (Solanum tuberosum L., cv. Desnitsa) and in its transformants with the native desA gene that encodes the acyl-lipid Δ12-desaturase from the cyanobacterium Synechocystis sp. PCC 6803 and with the hybrid desA gene fused to the reporter gene of thermostable lichenase (licBM3) from Clostridium thermocellum. Cold stress caused a rapid and significant increase in superoxide production and lipid peroxidation (the content of conjugated dienes and malonic dialdehyde) in wild-type plants. By contrast no significant increase was detected in transformed plants under cold stress conditions. This can be attributed to the fact that the overexpression of the acyl-lipid Δ12-desaturase in transformed potato plants promotes fatty acid polyunsaturation and presumably averts the accelerated generation of the superoxide anion, thus suppressing lipid peroxidation under low-temperature stress

Sign in / Sign up

Export Citation Format

Share Document