Altered gene expression in metastatic vs primary follicular thyroid carcinoma cell lines by cDNA microarray

2004 ◽  
Vol 11 (S2) ◽  
pp. S88-S88
Author(s):  
B. Xiang ◽  
L. Du ◽  
A. Kappes ◽  
J. Shilvansky ◽  
M. J. Hessner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Cdna Microarray ◽  
Carcinoma Cell ◽  
Follicular Thyroid Carcinoma ◽  
Altered Gene Expression ◽  
Carcinoma Cell Lines ◽  
Altered Gene ◽  
Thyroid Carcinoma Cell

scholarly journals Retinoic acid-mediated down-regulation of ENO1/MBP-1 gene products caused decreased invasiveness of the follicular thyroid carcinoma cell lines

2008 ◽  
Vol 42 (3) ◽  
pp. 249-260 ◽  
Author(s):  
Bogusz Trojanowicz ◽  
Anja Winkler ◽  
Kathrin Hammje ◽  
Zhouxun Chen ◽  
Carsten Sekulla ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Follicular Thyroid Carcinoma ◽  
Human Thyroid ◽  
Gene Products ◽  
Down Regulation ◽  
Carcinoma Cell Lines ◽  
Thyroid Carcinoma Cell

Retinoic acid (RA) acts as an anti-proliferative and redifferentiation agent in the therapy of thyroid carcinoma. Our previous studies demonstrated that pretreatment of follicular thyroid carcinoma cell lines FTC-133 and FTC-238 resulted in decreased in vitro proliferation rates and reduced tumor cell growth of xenotransplants. In addition to the previous results, we found that RA led to decreased vitality and invasiveness of FTC-133 and FTC-238 cells as they reacted with reduction of intracellular ATP levels and number of migrated cells respectively. However, the molecular mechanisms by which RA mediates these effects are not well understood. Two-dimensional (2D) screening of the proteins related to ATP metabolism and western blot analysis revealed α-enolase (ENO1) to be down-regulated in FTC-133 and FTC-238 cells after RA treatment. 2D gel detection and mass spectrometric analysis revealed that ENO1 existed as three separate protein spots of distinct pIs (ENO1–A1–A3). Comparative 2D difference gel electrophoresis analysis of fluorescently labeled protein samples of RA-treated and untreated FTC-133 demonstrated a selective down-regulation of ENO1-A1 which we identified as a phosphoprotein. RA caused the dephosphorylation of ENO1-A1. Both, RA-mediated and specific knock-down of ENO1/MBP-1 resulted in the reduction of MYC oncoprotein, and simultaneously decreased proliferation rates of FTC-133 and FTC-238 cell lines. In summary, the RA-mediated down-regulation of the ENO1 gene products and MYC oncoprotein provides a novel molecular mechanism facilitating the anti-proliferative effect of RA in human thyroid carcinoma cells and suggests new pathways for supportive RA therapies.

Degradation of Extracellular Matrix by Metastatic Follicular Thyroid Carcinoma Cell Lines: Role of the Plasmin Activation System

Thyroid ◽  
1999 ◽  
Vol 9 (9) ◽  
pp. 913-919 ◽  
Author(s):  
JAN W.A. SMIT ◽  
GABRI VAN DER PLUIJM ◽  
HANS A. ROMIJN ◽  
CLEMENS W.G.M. LÖWIK ◽  
HANS MORREAU ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cell Lines ◽  
Activation System ◽  
Thyroid Carcinoma Cell

scholarly journals Intracellular localization of the PDE4A cAMP-specific phosphodiesterase splice variant RD1 (RNPDE4A1A) in stably transfected human thyroid carcinoma FTC cell lines

1997 ◽  
Vol 321 (1) ◽  
pp. 177-185 ◽  
Author(s):  
Linda POOLEY ◽  
Yasmin SHAKUR ◽  
Graham RENA ◽  
Miles D. HOUSLAY
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Splice Variant ◽  
Carcinoma Cell ◽  
Follicular Thyroid Carcinoma ◽  
Intracellular Localization ◽  
Human Thyroid ◽  
Parent Cell ◽  
Carcinoma Cell Lines ◽  
Thyroid Carcinoma Cell

Cells of two human follicular thyroid carcinoma cell lines (FTC133, FTC236) were stably transfected with a cDNA encoding the PDE4A cAMP-specific phosphodiesterase (PDE) splice variant RD1 (RNPDE4A1A) so as to generate the cloned cell lines, FTC133A and FTC236A. This allowed the expression of a novel rolipram-inhibited cAMP-specific PDE activity in these cells. Unlike the parent cell lines in which Ca2+/calmodulin caused a profound activation (approx. 3–4-fold) of homogenate PDE activity, no such stimulation was evident in the RD1-expressing cell lines, indicating loss of PDE1 activity. Reverse transcriptase-PCR analysis indicated that this was due to the down-regulation of the PDE1C isoform. The novel PDE4 activity in transfected cells was located exclusively in the membrane fraction, as was immunoreactive RD1. Low concentrations of the detergent Triton X-100, but not high NaCl concentrations, allowed RD1 to be solubilized. Laser scanning confocal immunofluorescence analyses identified RD1 immunoreactivity in a discrete perinuclear region of these RD1-expressing transfected cell lines. A similar pattern of labelling was observed using the antiserum Tex1, which specifically identified the Golgi apparatus. Treatment of FTC133A cells with the Golgi-perturbing agents monensin and brefeldin A led to a similar redistribution of immunoreactive species detected using both the Tex1 and anti-RD1 antisera. It is suggested that the PDE4A splice variant RD1 contains a membrane-association signal which allows the targeted expression of RD1 within the Golgi complex of these human follicular thyroid carcinoma cell lines.

scholarly journals Expression pattern of the pendrin and sodium/iodide symporter genes in human thyroid carcinoma cell lines and human thyroid tumors

2001 ◽  
pp. 129-135 ◽  
Author(s):  
F Arturi ◽  
D Russo ◽  
JM Bidart ◽  
D Scarpelli ◽  
M Schlumberger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Human Thyroid ◽  
Sodium Iodide Symporter ◽  
Carcinoma Cell Lines ◽  
Nis Gene ◽  
Thyroid Carcinoma Cell ◽  
Pds Gene

OBJECTIVE: In the present study we analyzed the pattern of pendrin (PDS) and sodium/iodide symporter (NIS) gene expression in some thyroid carcinoma cell lines and a series of thyroid tumoral tissues. METHODS: Total RNA was extracted from all cell lines and from 53 tissues, and gene expression was examined by RT-PCR. Semiquantitative 'multiplex' RT-PCR was used to assess variations in PDS gene expression among various thyroid pathologies. Pendrin expression was determined in the thyroid cell lines by Western blot analysis. RESULTS: PDS mRNA was expressed in all the cells investigated; conversely, NIS mRNA was detectable only in the B-CPAP cells. Pendrin protein was expressed in B-CPAP and WRO cell lines, reduced in FRO and absent in ARO cells. PDS gene expression was not detected in 5 of 25 differentiated thyroid carcinomas (DTC) while NIS gene was not expressed in six carcinomas. A concordance expression of both PDS and NIS transcripts was found in 20 DTC. In contrast, 2 neoplastic thyroid tissues carrying undetectable PDS mRNA maintained NIS transcript, and 3 thyroid carcinomas negative for NIS mRNA retained the expression of PDS gene. A semiquantitative analysis showed that the mean PDS mRNA levels were significantly decreased in DTC tissues. CONCLUSIONS: Our data demonstrate that pendrin expression: (i) is present in the more differentiated thyroid carcinoma cell lines studied; (ii) is reduced or absent in DTC tissues; (iii) may not correlate with the NIS expression. These alterations may contribute to the loss of iodine concentration ability detected in thyroid tumors.

Role of Integrins in the Attachment of Metastatic Follicular Thyroid Carcinoma Cell Lines to Bone

Thyroid ◽  
1998 ◽  
Vol 8 (1) ◽  
pp. 29-36 ◽  
Author(s):  
JAN W.A. SMIT ◽  
GABRI van der PLUIJM ◽  
HANS J.M. VLOEDGRAVEN ◽  
CLEMENS W.G.M. LÖWIK ◽  
BERNARD M. GOSLINGS
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cell Lines ◽  
Thyroid Carcinoma Cell

scholarly journals In Vitro Identification and Characterization of CD133pos Cancer Stem-Like Cells in Anaplastic Thyroid Carcinoma Cell Lines

PLoS ONE ◽  
2008 ◽  
Vol 3 (10) ◽  
pp. e3544 ◽  
Author(s):  
Giovanni Zito ◽  
Pierina Richiusa ◽  
Alessandra Bommarito ◽  
Elvira Carissimi ◽  
Leonardo Russo ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cell Lines ◽  
Identification And Characterization ◽  
Thyroid Carcinoma Cell
Sign in / Sign up

Export Citation Format

Share Document