Three-dimensional changes of the cytoskeleton of vascular endothelial cells exposed to sustained hydrostatic pressure

1998 ◽  
Vol 36 (4) ◽  
pp. 520-527 ◽  
Author(s):  
S. A. Salwen ◽  
D. H. Szarowski ◽  
J. N. Turner ◽  
R. Bizios
Keyword(s):  
Endothelial Cells ◽  
Hydrostatic Pressure ◽  
Vascular Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Dimensional Changes

Interaction between vascular endothelial cells and vascular intimal spindle-shaped cells in vitro

1983 ◽  
Vol 60 (1) ◽  
pp. 89-102
Author(s):  
D de Bono ◽  
C. Green
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Pure Cultures ◽  
Species Specific ◽  
Endothelial Growth Factor

The interactions between human or bovine vascular endothelial cells and fibroblast-like vascular intimal spindle-shaped cells have been studied in vitro, using species-specific antibodies to identify the different components in mixed cultures. Pure cultures of endothelial cells grow as uniform, nonoverlapping monolayers, but this growth pattern is lost after the addition of spindle cells, probably because the extracellular matrix secreted by the latter causes the endothelial cells to modify the way they are attached to the substrate. The result is a network of tubular aggregates of endothelial cells in a three-dimensional ‘polylayer’ of spindle-shaped cells. On the other hand, endothelial cells added to growth-inhibited cultures of spindle-shaped cells will grow in sheets over the surface of the culture. Human endothelial cells grown in contact with spindle-shaped cells have a reduced requirement for a brain-derived endothelial growth factor. The interactions of endothelial cells and other connective tissue cells in vitro may be relevant to the mechanisms of endothelial growth and blood vessel formation in vivo, and emphasize the potential importance of extracellular matrix in controlling endothelial cell behaviour.

Induction of Apoptotic Cell Death in Vascular Endothelial Cells Cultured in Three-Dimensional Collagen Lattice

1999 ◽  
Vol 248 (2) ◽  
pp. 498-508 ◽  
Author(s):  
Masafumi Kuzuya ◽  
Shosuke Satake ◽  
Miguel A. Ramos ◽  
Shigeru Kanda ◽  
Teruhiko Koike ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Death ◽  
Vascular Endothelial Cells ◽  
Apoptotic Cell ◽  
Three Dimensional ◽  
Apoptotic Cell Death ◽  
Vascular Endothelial ◽  
Collagen Lattice ◽  
Cells Cultured

Angiogenic Stimuli Are Essential for Survival of Vascular Endothelial Cells in Three-Dimensional Collagen Lattice

1998 ◽  
Vol 244 (3) ◽  
pp. 642-646 ◽  
Author(s):  
Shosuke Satake ◽  
Masafumi Kuzuya ◽  
Miguel A. Ramos ◽  
Shigeru Kanda ◽  
Akihisa Iguchi
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Collagen Lattice

Hyaluronic acid and α-elastin based hydrogel for three dimensional culture of vascular endothelial cells

2018 ◽  
Vol 46 ◽  
pp. 28-33 ◽  
Author(s):  
Calogero Fiorica ◽  
Fabio S. Palumbo ◽  
Giovanna Pitarresi ◽  
Mario Allegra ◽  
Roberto Puleio ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Hyaluronic Acid ◽  
Vascular Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Three Dimensional Culture
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