Modelling the growth and protein production by insect cells following infection by a recombinant baculovirus in suspension culture

1992 ◽  
Vol 9 (1-3) ◽  
pp. 149-155 ◽  
Author(s):  
J. Power ◽  
P. F. Greenfield ◽  
L. Nielsen ◽  
S. Reid
Keyword(s):  
Suspension Culture ◽  
Protein Production ◽  
Insect Cells ◽  
Recombinant Baculovirus

scholarly journals FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing

2018 ◽  
Author(s):  
Régis P. Lemaitre ◽  
Aliona Bogdanova ◽  
Barbara Borgonovo ◽  
Jeffrey B. Woodruff ◽  
David N. Drechsel
Keyword(s):  
Growth Factors ◽  
Protein Production ◽  
Insect Cells ◽  
Coiled Coil ◽  
Recombinant Baculovirus ◽  
Low Complexity ◽  
Proteolytic Processing ◽  
Vector System ◽  
Fluorescent Markers ◽  
Family Growth

ABSTRACTBaculovirus-mediated expression in insect cells is a powerful approach for protein production. However, many existing methods are time consuming, offer limited options for protein tagging, and are unsuitable for secreted proteins requiring proteolytic maturation, such as TGF-β family growth factors. To overcome these limitations, we engineered “FlexiBAC”, a system that simplifies baculovirus production and permits furin-driven proteolytic maturation of targets. This system allows recombinant baculovirus formation inside insect cells and reduces the time between initial cloning and protein production to 13 days. FlexiBAC includes 146 shuttle vectors that append combinations of purification tags, fluorescent markers, proteolytic cleavage sites, trafficking signals, and chemical conjugation tags to the termini of the target protein. We demonstrate that this system can be used to produce high levels of mature, active forms of TGF-β family growth factors, such as Activin A, as well as other proteins that are typically difficult to reconstitute, such as proteins rich in coiled-coil, low complexity, and disordered domains.

Expression of the human multidrug transporter in insect cells by a recombinant baculovirus

Biochemistry ◽  
1990 ◽  
Vol 29 (9) ◽  
pp. 2295-2303 ◽  
Author(s):  
Ursula A. Germann ◽  
Mark C. Willingham ◽  
Ira Pastan ◽  
Michael M. Gottesman
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Multidrug Transporter

High-Level Expression of Biologically Active Human Prolactin from Recombinant Baculovirus in Insect Cells

2000 ◽  
Vol 20 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Tapas Das ◽  
Paul W. Johns ◽  
Vincent Goffin ◽  
Paul Kelly ◽  
Bruce Kelder ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Biologically Active ◽  
High Level Expression ◽  
Human Prolactin ◽  
High Level

High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus

1994 ◽  
Vol 136 (3-4) ◽  
pp. 375-380 ◽  
Author(s):  
S. J. Edwards ◽  
M. B. Hayden ◽  
R. C. Hamilton ◽  
J. A. Haynes ◽  
I. T. Nisbet ◽  
...  
Keyword(s):  
Insect Cells ◽  
Recombinant Baculovirus ◽  
High Level ◽  
Level Production

Industrial Large Scale of Suspension Culture of Insect Cells

2014 ◽  
pp. 348-389 ◽  
Author(s):  
António Roldão ◽  
Manon Cox ◽  
Paula Alves ◽  
Manuel Carrondo ◽  
Tiago Vicente
Keyword(s):  
Suspension Culture ◽  
Large Scale ◽  
Insect Cells
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