Design and performance of a trickle-bed bioreactor with immobilized hybridoma cells

1992 ◽  
Vol 9 (1-3) ◽  
pp. 29-40 ◽  
Author(s):  
H. A. Phillips ◽  
J. M. Scharer ◽  
N. C. Bols ◽  
M. Moo-Young
Keyword(s):  
Hybridoma Cells ◽  
Trickle Bed ◽  
And Performance ◽  
Trickle Bed Bioreactor

UJI KINERJA PADA PROSES PRODUKSI ETANOL MELALUI KOMBINASI PROSES FERMENTASI DAN STRIPPING

EKUILIBIUM ◽  
2012 ◽  
Vol 11 (2) ◽  
Author(s):  
Margono Margono
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Renewable Energy ◽  
Production Process ◽  
Ethanol Production ◽  
Anaerobic Fermentation ◽  
Fermentation Medium ◽  
High Price ◽  
Trickle Bed ◽  
Trickle Bed Bioreactor ◽  
Biofilm Development

<p><strong><em>Abstract:</em></strong> <em>Renewable energy necesity have promote research on ethanol production technology. Ethanol is the potential renewable energy substituting gasoline. However, the conventional problem is high price of the ethanol. The objective of this research was to test the performance of alternative process in producing ethanol, i.e. combination of fermentation process with ethanol stripping in trickle bed bioreactor. The experimental was using Saccharomyces cerevisiae FNCC 3012 and sugarcane bagass as bed particle. It was devided into 2 process steps of biofilm development and ethanol production. Biofilm development was done by circulating medium in bioreactor aerobically. Duration of the biofilm development was 24 hours and followed by ethanol production step which was combinating anaerobic fermentation and stripping process using nitrogen. Production process was conducted for 36 hours lifetime. This method resulted biofilm developing in fermentation medium, not on baggas surfaces. Consequently, ethanol production happened in circulated fermentation medium. The productivity of this method of ethanol production process was not better than the conventional process. Neverherless, the experimental showed that the product stripping and fermentation could be done simultaneously. The stripping process increased ethanol product concentration up to 25% higher than in the broth</em>.</p><p> <strong><em>Keywords:</em></strong> <em>ethanol, Saccharomyces cerevisiae FNCC 3012, trickle bed bioreactor, stripping, biofilm</em></p>

A novel model for continuous fermentation process for Worcestershire sauce production using a trickle bed bioreactor

1996 ◽  
Vol 81 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Tetsuya Fukaya ◽  
Yoshiya Furuta ◽  
Yukio Ishiguro ◽  
Hiroyuki Horitsu ◽  
Kazuhiro Takamizawa
Keyword(s):  
Fermentation Process ◽  
Continuous Fermentation ◽  
Trickle Bed ◽  
Trickle Bed Bioreactor ◽  
Novel Model

scholarly journals Bacterial Community Dynamics during Start-Up of a Trickle-Bed Bioreactor Degrading Aromatic Compounds

1998 ◽  
Vol 64 (3) ◽  
pp. 930-939 ◽  
Author(s):  
Marion Stoffels ◽  
Rudolf Amann ◽  
Wolfgang Ludwig ◽  
Dariusch Hekmat ◽  
Karl-Heinz Schleifer
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Aromatic Compounds ◽  
Community Dynamics ◽  
23S Rrna ◽  
Significant Shift ◽  
Wastewater Sample ◽  
Bacterial Community Dynamics ◽  
Trickle Bed ◽  
Trickle Bed Bioreactor

ABSTRACT This study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (Solvesso100). The starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a car painting facility as the inoculum and Solvesso100 as the sole carbon source. The bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotides. Two significant shifts in the bacterial community structure could be demonstrated. The original inoculum from the wastewater of the car factory was rich in proteobacteria of the alpha and beta subclasses, while the final fermentor enrichment was dominated by bacteria closely related toPseudomonas putida or Pseudomonas mendocina, which both belong to the gamma subclass of the classProteobacteria. A second significant shift was observed when the fermentor culture was transferred as inoculum to the trickle-bed bioreactor. The community structure in the bioreactor gradually returned to a higher complexity, with the dominance of beta and alpha subclass proteobacteria, whereas the gamma subclass proteobacteria sharply declined. Obviously, the preceded pollutant adaptant did not lead to a significant enrichment of bacteria that finally dominated in the trickle-bed bioreactor. In the course of experiments, three new 16S as well as 23S rRNA-targeted probes for beta subclass proteobacteria were designed, probe SUBU1237 for the generaBurkholderia and Sutterella, probe ALBO34a for the genera Alcaligenes and Bordetella, and probe Bcv13b for Burkholderia cepacia andBurkholderia vietnamiensis. Bacteria hybridizing with the probe Bcv13b represented the main Solvesso100-degrading population in the reactor.

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