The use of body hydrogen as an internal standard in the measurement of nitrogen in vivo by prompt neutron capture gamma-ray analysis

1979 ◽  
Vol 48 (1-2) ◽  
pp. 243-252 ◽  
Author(s):  
D. Vartsky ◽  
W. V. Prestwich ◽  
B. J. Thomas ◽  
J. T. Dabek ◽  
D. R. Chettle ◽  
...  
Keyword(s):  
Neutron Capture ◽  
Gamma Ray ◽  
Internal Standard ◽  
Prompt Neutron

In vivo determination of protein by prompt neutron capture in fibrocystic disease

1985 ◽  
Author(s):  
B. J. Allen ◽  
N. Blagojevic ◽  
K. Gaskin ◽  
V. Soutter ◽  
R. Howman-Giles
Keyword(s):  
Neutron Capture ◽  
Prompt Neutron ◽  
Fibrocystic Disease

scholarly journals Dosimetry of In Vivo Experiment for Lung Cancer Based on Boron Neutron Capture Therapy on Radial Piercing Beam Port Kartini Nuclear Reactor by MCNPX Simulation Method

2018 ◽  
Vol 35 (3) ◽  
pp. 213-216
Author(s):  
Atika Maysaroh ◽  
Kusminarto Kusminarto ◽  
Dwi Satya Palupi ◽  
Yohannes Sardjono
Keyword(s):  
Lung Cancer ◽  
Boron Neutron Capture Therapy ◽  
Neutron Capture ◽  
Boron Concentration ◽  
Gamma Ray ◽  
Neutron Capture Therapy ◽  
Thermal Neutrons ◽  
Boron Neutron Capture ◽  
Beam Port

Cancer is one of the leading causes of death globally, with lung cancer being among the most prevalent. Boron Neutron Capture Therapy (BNCT) is a cancer therapy method that uses the interaction between thermal neutrons and boron-10 which produces a decaying boron-11 particle and emits alpha, lithium 7 and gamma particles. A study was carried out to model an in vivo experiment of rat organisms that have lung cancer. Dimensions of a rat’s body were used in Konijnenberg research. Modeling lung cancer type, non-small cell lung cancer, was used in Monte Carlo N Particle-X. Lung cancer was modeled with a spherical geometry consisting of 3 dimensions: PTV, GTV, and CTV. In this case, the neutron source was from the radial piercing beam port of Kartini Reactor, Yogyakarta. The variation of boron concentration was 20, 25, 30, 35, 40, and 40 µg/g cancer. The output of the MCNP calculation was neutron scattering dose, gamma-ray dose and neutron flux from the reactor. A neutron flux was used to calculate the alpha proton and gamma-ray dose from the interaction of tissue material and thermal neutrons. The total dose was calculated from a four-dose component in BNCT. The results showed that the dose rate will increase when the boron concentration is higher, whereas irradiating time will decrease.

scholarly journals The Design and Preclinical Evaluation of a Single-Label Bimodal Nanobody Tracer for Image-Guided Surgery

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 360
Author(s):  
Pieterjan Debie ◽  
Noemi B. Declerck ◽  
Danny van Willigen ◽  
Celine M. Huygen ◽  
Bieke De Sloovere ◽  
...  
Keyword(s):  
Single Molecule ◽  
Gamma Ray ◽  
Nuclear Imaging ◽  
Primary Amines ◽  
Resection Margins ◽  
Fluorescent Light ◽  
Single Structure ◽  
Fluorescence Images

Intraoperative guidance using targeted fluorescent tracers can potentially provide surgeons with real-time feedback on the presence of tumor tissue in resection margins. To overcome the limited depth penetration of fluorescent light, combining fluorescence with SPECT/CT imaging and/or gamma-ray tracing has been proposed. Here, we describe the design and preclinical validation of a novel bimodal nanobody-tracer, labeled using a “multifunctional single attachment point” (MSAP) label, integrating a Cy5 fluorophore and a diethylenetriaminepentaacetic acid (DTPA) chelator into a single structure. After conjugation of the bimodal MSAP to primary amines of the anti-HER2 nanobody 2Rs15d and 111In-labeling of DTPA, the tracer’s characteristics were evaluated in vitro. Subsequently, its biodistribution and tumor targeting were assessed by SPECT/CT and fluorescence imaging over 24 h. Finally, the tracer’s ability to identify small, disseminated tumor lesions was investigated in mice bearing HER2-overexpressing SKOV3.IP1 peritoneal lesions. [111In]In-MSAP.2Rs15d retained its affinity following conjugation and remained stable for 24 h. In vivo SPECT/CT and fluorescence images showed specific uptake in HER2-overexpressing tumors with low background. High tumor-to-muscle ratios were obtained at 1h p.i. and remained 19-fold on SPECT/CT and 3-fold on fluorescence images over 24 h. In the intraperitoneally disseminated model, the tracer allowed detection of larger lesions via nuclear imaging, while fluorescence enabled accurate removal of submillimeter lesions. Bimodal nuclear/fluorescent nanobody-tracers can thus be conveniently designed by conjugation of a single-molecule MSAP-reagent carrying a fluorophore and chelator for radioactive labeling. Such tracers hold promise for clinical applications.

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