Intestinal ischemia-induced changes in the activity of NADPH-d and energy metabolism enzymes in rat myenteric and splanchnic plexus

1999 ◽  
Vol 31 (6) ◽  
pp. 376-380
Author(s):  
V. I. Lapsha ◽  
V. N. Bocharova ◽  
L. N. Utkina ◽  
V. N. Gurin
Keyword(s):  
Energy Metabolism ◽  
Intestinal Ischemia ◽  
Metabolism Enzymes ◽  
Induced Changes

ConA induced changes in energy metabolism of rat thymocytes

1992 ◽  
Vol 12 (5) ◽  
pp. 381-386 ◽  
Author(s):  
F. Buttgereit ◽  
M. D. Brand ◽  
M. Müller
Keyword(s):  
Protein Synthesis ◽  
Oxygen Consumption ◽  
Energy Metabolism ◽  
Dna Synthesis ◽  
Atp Synthesis ◽  
Proton Leak ◽  
Ehrlich Ascites ◽  
Activated State ◽  
Ehrlich Ascites Tumour ◽  
Induced Changes

The influence of ConA on the energy metabolism of quiescent rat thymocytes was investigated by measuring the effects of inhibitors of protein synthesis, proteolysis, RNA/DNA synthesis, Na+K+-ATPase, Ca2+-ATPase and mitochondrial ATP synthesis on respiration. Only about 50% of the coupled oxygen consumption of quiescent thymocytes could be assigned to specific processes using two different media. Under these conditions the oxygen is mainly used to drive mitochondrial proton leak and to provide ATP for protein synthesis and cation transport, whereas oxygen consumption to provide ATP for RNA/DNA synthesis and ATP-dependent proteolysis was not measurable. The mitogen ConA produced a persistent increase in oxygen consumption by about 30% within seconds. After stimulation more than 80% of respiration could be assigned to specific processes. The major oxygen consuming processes of ConA-stimulated thymocytes are mitochondrial proton leak, protein synthesis and Na+K+-ATPase with about 20% each of total oxygen consumption, while Ca2+-ATPase and RNA/DNA synthesis contribute about 10% each. Quiescent thymocytes resemble resting hepatocytes in that most of the oxygen consumption remains unexplained. In constrast, the pattern of energy metabolism in stimulated thymocytes is similar to that described for Ehrlich Ascites tumour cells and splenocytes, which may also be in an activated state. Most of the oxygen consumption is accounted for, so the unexplained process(es) in unstimulated cells shut(s) off on stimulation.

ConA induced changes in energy metabolism of rat thymocytes

1992 ◽  
Vol 12 (2) ◽  
pp. 109-114 ◽  
Author(s):  
F. Buttgereit ◽  
M. D. Brand ◽  
M. Müller
Keyword(s):  
Protein Synthesis ◽  
Oxygen Consumption ◽  
Energy Metabolism ◽  
Dna Synthesis ◽  
Atp Synthesis ◽  
Proton Leak ◽  
Ehrlich Ascites ◽  
Activated State ◽  
Ehrlich Ascites Tumour ◽  
Induced Changes

The influence of ConA on the energy metabolism of quiescent rat thymocytes was investigated by measuring the effects of inhibitors of protein synthesis, proteolysis, RNA/DNA synthesis, Na+K+-ATPase, Ca2+-ATPase and mitochondrial ATP synthesis on respiration. Only about 50% of the coupled oxygen consumption of quiescent thymocytes could be assigned to specific processes using two different media. Under these conditions the oxygen is mainly used to drive mitochondrial proton leak and to provide ATP for protein synthesis and cation transport, whereas oxygen consumption to provide ATP for RNA/DNA synthesis and ATP-dependent proteolysis was not measurable. The mitogen ConA produced a persistent increase in oxygen consumption by about 30% within seconds. After stimulation more than 80% of respiration could be assigned to specific processes. The major oxygen consuming processes of ConA-stimulated thymocytes are mitochondrial proton leak, protein synthesis and Na+K+-ATPase with about 20% each of total oxygen consumption, while Ca2+-ATPase and RNA/DNA synthesis contribute about 10% each. Quiescent thymocytes resemble resting hepatocytes in that most of the oxygen consumption remains unexplained. In contrast, the pattern of energy metabolism in stimulated thymocytes is similar to that described for Ehrlich Ascites tumour cells and splenocytes, which may also be in an activated state. Most of the oxygen consumption is accounted for, so the unexplained process(es) in unstimulated cells shut(s) off on stimulation.

Aspirin Induced Changes in Enzymes of Colonic Energy Metabolism and ATPases of Rats Exposed to Cycas and Fed a Nigerian-Like Diet

2007 ◽  
Vol 7 (8) ◽  
pp. 1250-1258
Author(s):  
G.E. Eriyamremu ◽  
E.C. Onyeneke . ◽  
N.J. Orhue . ◽  
S.I. Ojeaburu . ◽  
S.O. Uanseoje . ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Induced Changes

scholarly journals Hepatic Cystathionine β-Synthase Activity is Increased by Greater Post-ruminal Supply of Methionine During the Periparturient Period in Dairy Cows

2019 ◽  
Author(s):  
Mario Vailati-Riboni ◽  
Fernanda Batistel ◽  
Rainie R C S Yambao ◽  
Claudia Parys ◽  
Yuan-Xiang Pan ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Dairy Cows ◽  
Carbon Metabolism ◽  
Production Efficiency ◽  
Fumarate Hydratase ◽  
Mrna Abundance ◽  
Periparturient Period ◽  
Inflammatory Status ◽  
Metabolism Enzymes ◽  
One Carbon Metabolism

Abstract Background Post-ruminal supply of Met during the periparturient period enhances production efficiency (feed conversion to milk) in dairy cows partly through alleviation of oxidant and inflammatory status. Whether alterations in hepatic one-carbon (major contributor of antioxidants) and/or energy-metabolism contribute to these beneficial effects is unknown. Objectives Investigate alterations in hepatic one-carbon and energy metabolism and associations with plasma amino acids (AA) and production efficiency in response to enhanced post-ruminal supply of Met. Methods Holstein cows (n = 30/group) were fed during the last 28 days of pregnancy a control diet (CON) or the control plus ethyl-cellulose rumen-protected methionine (MET; 0.9 g·kg−1 of dry matter intake). Plasma (n = 15/group) and liver tissue (n = 10/group) were collected throughout the periparturient period to evaluate AA profiles, activity of TCA cycle, and one-carbon metabolism via mRNA abundance, enzyme activity, and targeted metabolomics. Results Cows in MET had greater overall (27%, P = 0.027) plasma Met concentrations, but had similar total plasma AA concentrations. Although mRNA abundance of one-carbon metabolism enzymes did not differ, hepatic activity of cystathionine β-synthase (CBS) (51.2 vs. 44.4 mmol·h−1·mg−1 protein; P = 0.032) and concentration (19%, P = 0.048) of the cellular antioxidant glutathione were greater overall in MET. mRNA abundance of aconitase 2 and fumarate hydratase was greater overall (P = 0.049), and phosphoenolpyruvate carboxykinase 1 tended (P = 0.093) to be greater overall in cows fed MET. There was a tendency (P ≤ 0.093) for greater overall hepatic concentrations of malic acid, α-ketoglutaric acid, and isocitric acid in cows fed MET. Conclusions Greater activity of CBS in response to enhanced post-ruminal supply of Met likely contributes to alleviating oxidant status by increasing concentrations of glutathione. Hence, transsulfuration plays an important role in the observed improvements in production efficiency of dairy cows during the periparturient period.

scholarly journals Bilirubin-Induced Changes in Brain Energy Metabolism after Osmotic Opening of the Blood-Brain Barrier

1991 ◽  
Vol 30 (5) ◽  
pp. 473-478 ◽  
Author(s):  
Richard P Wennberg ◽  
Barbro B Johansson ◽  
Jaroslava Folbergrova ◽  
Bo K Siesjö
Keyword(s):  
Energy Metabolism ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Brain Energy Metabolism ◽  
Blood Brain ◽  
Induced Changes ◽  
Brain Energy

scholarly journals In SilicoSearch of Energy Metabolism Inhibitors for Alternative Leishmaniasis Treatments

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Lourival A. Silva ◽  
Marina C. Vinaud ◽  
Ana Maria Castro ◽  
Pedro Vítor L. Cravo ◽  
José Clecildo B. Bezerra
Keyword(s):  
Developing Countries ◽  
Energy Metabolism ◽  
Complex Disease ◽  
Current Treatment ◽  
New Drugs ◽  
Socioeconomic Impact ◽  
Search Criterion ◽  
Metabolism Enzymes ◽  
Target Database ◽  
Specific Inhibitors

Leishmaniasis is a complex disease that affects mammals and is caused by approximately 20 distinct protozoa from the genusLeishmania. Leishmaniasis is an endemic disease that exerts a large socioeconomic impact on poor and developing countries. The current treatment for leishmaniasis is complex, expensive, and poorly efficacious. Thus, there is an urgent need to develop more selective, less expensive new drugs. The energy metabolism pathways ofLeishmaniainclude several interesting targets for specific inhibitors. In the present study, we sought to establish which energy metabolism enzymes inLeishmaniacould be targets for inhibitors that have already been approved for the treatment of other diseases. We were able to identify 94 genes and 93Leishmaniaenergy metabolism targets. Using each gene’s designation as a search criterion in the TriTrypDB database, we located the predicted peptide sequences, which in turn were used to interrogate the DrugBank, Therapeutic Target Database (TTD), and PubChem databases. We identified 44 putative targets of which 11 are predicted to be amenable to inhibition by drugs which have already been approved for use in humans for 11 of these targets. We propose that these drugs should be experimentally tested and potentially used in the treatment of leishmaniasis.

scholarly journals Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment

Cell Research ◽  
2007 ◽  
Vol 17 (8) ◽  
pp. 689-700 ◽  
Author(s):  
Xiao-Lan Chen ◽  
Gang Lu ◽  
Ying-Xia Gong ◽  
Liang-Cai Zhao ◽  
Jie Chen ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Morphine Treatment ◽  
Chronic Morphine ◽  
Metabolism Enzymes ◽  
Chronic Morphine Treatment
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