Glial fibrillary acidic protein in the brain of prenatally stressed rats

1999 ◽  
Vol 31 (3) ◽  
pp. 207-208
Author(s):  
T. I. Duka ◽  
V. I. Chernaya
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
Prenatally Stressed ◽  
The Brain

Differential patterns of glial fibrillary acidic protein-immunolabeling in the brain of adult lizards

2003 ◽  
Vol 464 (2) ◽  
pp. 159-171 ◽  
Author(s):  
Samir Ahboucha ◽  
Abdelhadi Laalaoui ◽  
Marianne Didier-Bazes ◽  
Michelle Montange ◽  
Howard Michael Cooper ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
The Brain

scholarly journals P-glycoprotein is strongly expressed in the luminal membranes of the endothelium of blood vessels in the brain

1997 ◽  
Vol 326 (2) ◽  
pp. 539-544 ◽  
Author(s):  
Édith BEAULIEU ◽  
Michel DEMEULE ◽  
Lucian GHITESCU ◽  
Richard BÉLIVEAU
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Western Blotting ◽  
Plasma Membranes ◽  
Collagen Iv ◽  
Acidic Protein ◽  
Brain Capillary ◽  
P Glycoprotein ◽  
Cell Plasma ◽  
Capillary Endothelial Cells ◽  
The Brain

Luminal membranes of the vascular endothelium were isolated from brain, heart and lungs by modification of their density. The presence of P-glycoprotein (P-gp) was detected by Western blotting in luminal membranes from the endothelium of the three tissues. Strong enrichment in brain capillary luminal membranes, compared with brain capillaries (17-fold) and whole membranes (400–500-fold), indicates that P-gp is mainly located on the luminal side of the brain endothelium. Western blotting was also performed with antibodies directed against GLUT1, glial fibrillary acidic protein, adaptin, IP3R-3, integrins αv and collagen IV as controls to determine whether the preparations were contaminated by other membranes. Strong enrichment of GLUT1 in brain capillary luminal membranes (9.9-fold) showed that the preparation consisted mainly of endothelial cell plasma membranes. Poor enrichment of glial fibrillary acidic protein (1.4-fold) and adaptin (2.4-fold) and a decreased level of IP3R-3, integrins αv and collagen IV excludes the possibility of major contamination by astrocytes or internal and anti-luminal membranes. High levels of P-gp in the luminal membranes of brain capillary endothelial cells suggests that it may play an important role in limiting the access of anti-cancer drugs to the brain.

Expression of Nestin and Glial Fibrillary Acidic Protein in the Marginal Ischemic Zone of the Brain in SHR Rats

2020 ◽  
Vol 169 (4) ◽  
pp. 576-581
Author(s):  
D. L. Tsyba ◽  
O. V. Kirik ◽  
M. E. Kolpakova ◽  
A. A. Yakovleva ◽  
D. E. Korzhevskii
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
Shr Rats ◽  
The Brain

Glial fibrillary acidic protein in the brain of the caecilian

1995 ◽  
Vol 283 (1) ◽  
pp. 51 ◽  
Author(s):  
Christiane Naujoks-Manteuffel ◽  
Dietrich L. Meyer
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
The Brain

Glucocorticoids regulate the concentration of glial fibrillary acidic protein throughout the brain

1989 ◽  
Vol 494 (1) ◽  
pp. 159-161 ◽  
Author(s):  
James P. O'Callaghan ◽  
Roberta E. Brinton ◽  
Bruce S. McEwen
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
The Brain

scholarly journals Local industrial pollution induces astrocyte cytoskeleton rearrangement in the dice snake brain: GFAP as a biomarker

2020 ◽  
Vol 28 (3) ◽  
pp. 250-256
Author(s):  
V. Y. Gasso ◽  
A. N. Hahut ◽  
S. V. Yermolenko ◽  
I. A. Hasso ◽  
C. A. Agca ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Cell Biology ◽  
Industrial Pollution ◽  
Control Site ◽  
Acidic Protein ◽  
Molecular Characteristics ◽  
Environmental Toxicity ◽  
Site Characteristic ◽  
Pollution Exposure ◽  
The Brain

The present study was designed to evaluate the responsiveness of modulation of glial fibrillary acidic protein (GFAP) content and its fragmentation in the snake brain as a biomarker of local industrial pollution of aquatic ecosystems. Despite GFAP being a well known cytoskeleton marker of astrocytes’ reactivity in the brain of vertebrates, its expression in the snake brain remains insufficiently described. The GFAP expression and its fragmentation were detected using the immunoblot method in the snake brain. ROS level was determined with dichlorofluorescein diacetate fluorescence. The content of the glial fibrillary acidic protein (GFAP) of filament (cytoskeleton) and soluble (cytosol) fractions in the brain of dice snake Natrix tessellata from three ecosystems with different rates of industrial pollution were studied (two polluted and one clean control site). Characteristic increase in GFAP fragmentation was noted for the snakes from both the researched polluted sites. Significant increase in the content of the GFAP cleaved polypeptide fragments induced by industrial pollution exposure was confirmed in the snakes’ brains. Meaningful GFAP fragmentation was determined in snake brain astrocytes as an increase in cleaved fragments of 47–35 kDa molecular weight for both soluble and cytoskeletal GFAP fractions. We found significant abnormality in the ratio of the GFAP soluble fraction to the cytoskeletal one in contaminant-exposed dice snakes. It should testify to significant metabolic disturbance in nerve cells of the dice snakes. Furthermore, growth of reactive oxygen species level as the main cause of oxidative stress was determined in brains of the snakes exposed to environmental toxicity. Thus, astrocyte cytoskeleton disorders are associated with pollutant-induced redox imbalance in the snake brain. Despite the limited data on glial cell biology in the reptilian brain, the observed results prove that snake astrocytes can respond to the environmental toxicity using typical astroglial response. The presented results evidence that monitoring of molecular characteristics of glial cytoskeleton in dice snakes could be used as reliable biomarker of neurotoxicity and adverse effects of industrial pollution. Further studies are required to elucidate the role of astrocyte cytoskeleton in the response against neurotoxic contaminants.

Increased Expression of Glial Fibrillary Acidic Protein in the Brain of Spontaneously Hypertensive Rats

2004 ◽  
Vol 26 (4) ◽  
pp. 335-350 ◽  
Author(s):  
Daniele Tomassoni ◽  
Roberto Avola ◽  
Maria Antonietta Di Tullio ◽  
Maurizio Sabbatini ◽  
Lucia Vitaioli ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Spontaneously Hypertensive Rats ◽  
Acidic Protein ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
The Brain
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