Assessment of serum bone alkaline phosphatase activity with a new elisa using a specific monoclonal antibody

1996 ◽  
Vol 6 (S1) ◽  
pp. 189-189
Author(s):  
P. Garnero ◽  
C. Chervet ◽  
P D Delmas
Keyword(s):  
Monoclonal Antibody ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Alkaline Phosphatase ◽  
Specific Monoclonal Antibody ◽  
Serum Bone Alkaline Phosphatase

The influence of ‘hypophysectomy’ on bone growth and alkaline phosphatase activity in the chick embryo

Development ◽  
1973 ◽  
Vol 29 (3) ◽  
pp. 515-527
Author(s):  
D. J. McWhinnie ◽  
Robert C. Thommes
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Growth ◽  
Developmental Time ◽  
Bone Alkaline Phosphatase ◽  
Enzyme Synthesis ◽  
Skeletal Maturation ◽  
Specific Alkaline Phosphatase ◽  
Normal Increase

Wet weights, total bone alkaline phosphatase activity and specific alkaline phosphatase activity were determined on demarrowed femurs of normal, ‘hypophysectomized’ and pituitary-grafted chick embryos at selected intervals of incubation. In normal bones, all parameters noted above increased progressively through developmental time. ‘Hypophysectomy’ by means of surgical decapitation significantly retarded the normal increase of femur wet weight, total and specific alkaline phosphatase activity; in embryos bearing pituitary transplants, there was a return towards normal values. The possible role(s) of the pituitary in skeletal maturation and enzyme synthesis or activation is discussed.

Multicenter evaluation of Iso-ALP test kit for measurement of bone alkaline phosphatase activity in serum and plasma

1993 ◽  
Vol 39 (4) ◽  
pp. 648-652 ◽  
Author(s):  
S B Rosalki ◽  
A Y Foo ◽  
A Burlina ◽  
W Prellwitz ◽  
P Stieber ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Alkaline Phosphatase ◽  
Alp Activity ◽  
Test Kit ◽  
Reference Limits ◽  
Bone Fraction ◽  
Adults And Children ◽  
Wheat Germ Lectin

Abstract A test kit (Iso-ALP, Boehringer Mannheim) for measuring human bone alkaline phosphatase activity in serum or plasma was evaluated in five laboratories in three countries. The assay is based on the principle described by Rosalki and Foo (Clin Chem 1984;30:1182-6) and uses wheat germ lectin to precipitate bone alkaline phosphatase. Residual ALP in the supernate in comparison with total ALP is used to quantify the bone fraction. The imprecision of residual ALP measurement was low (median between-run CV 4.9%) and comparable with that of total ALP. Linearity of precipitation was demonstrable up to a bone ALP activity (diethanolamine buffer 37 degrees C) of 2000 U/L, though a matrix effect was observed for dilutions of high-activity sera in saline or bovine serum albumin. For assaying patients' samples, different batches of lectin demonstrated excellent comparability. Taking electrophoresis as a basis for standardization, we determined that the lectin precipitated approximately 90% of bone ALP, but < 5% of nonbone ALP. From this we derived serum/plasma upper reference limits for bone ALP activity in adults and children.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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