Tracking cell invasion of human glioma cells and suppression by anti-matrix metalloproteinase agent in rodent brain-slice model

2002 ◽  
Vol 19 (2) ◽  
pp. 69-76 ◽  
Author(s):  
Daizo Yoshida ◽  
Kunihiro Watanabe ◽  
Masahiro Noha ◽  
Hiroshi Takahashi ◽  
Akira Teramoto ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Cell Invasion ◽  
Brain Slice ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Rodent Brain

The effect of exogenous gangliosides on matrix metalloproteinase secretion by human glioma cells in vitro

1996 ◽  
Vol 32 (5) ◽  
pp. 868-871 ◽  
Author(s):  
S.L. Maidment ◽  
A. Merzak ◽  
S. Koochekpour ◽  
H.K. Rooprai ◽  
G.J. Rucklidge ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells

scholarly journals Suppression of matrix metalloproteinase-2 gene expression and invasion in human glioma cells by MMAC/PTEN

Oncogene ◽  
2001 ◽  
Vol 20 (46) ◽  
pp. 6669-6678 ◽  
Author(s):  
Dimpy Koul ◽  
Ranjani Parthasarathy ◽  
Ruijun Shen ◽  
Michael A Davies ◽  
Samar A Jasser ◽  
...  
Keyword(s):  
Gene Expression ◽  
Matrix Metalloproteinase ◽  
Glioma Cells ◽  
Matrix Metalloproteinase 2 ◽  
Human Glioma ◽  
Human Glioma Cells

P16.05 Implementation of a novel ex-vivo brain slice model to study human glioblastoma and glioma-associated microglia

2021 ◽  
Vol 23 (Supplement_2) ◽  
pp. ii56-ii57
Author(s):  
C Flüh ◽  
C Nanvuma ◽  
Y Huang ◽  
E Motta ◽  
L Kuhrt ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Brain Slice ◽  
Tumor Volume ◽  
Ex Vivo ◽  
Glioma Cells ◽  
Body Volume ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Process Length ◽  
Murine Glioma

Abstract BACKGROUND Glioblastoma multiforme is a highly malignant brain tumor with a devastating prognosis. Resection followed by radio-chemotherapy leads to an overall survival of only 15 months. Up to 40% of the tumor mass consist of tumor-associated microglia and macrophages (TAMs). These cells were shown to promote tumor growth and invasiveness in many murine glioma models. The interaction between TAMs and tumor cells is crucial for tumor progression and includes several known pathways. Still, murine glioma models only partially mirror the human tumor microenvironment. Several known genes, which are highly upregulated in human glioma and TAMs are only expressed in human tissue and not in mice. To further investigate some of these genes, we aimed at establishing a humanized ex-vivo brain slice model, in which human TAMs and human glioma cells can be studied in a standardized manner. MATERIAL AND METHODS We used 250 micrometer thick murine brain slices, which were depleted of intrinsic microglia by applying clodoronated liposomes. Next, we inoculated human glioma cells (originating from the cell lines mCherryU87, mCherryU251MG, mCherryLN229 and several patient derived cells lines) with or without human microglia derived from induced pluripotent stem cells (iPSCs). Slices were cultivated for 7 to 14 days. Next, we performed a detailed analysis of microglia morphology (sphericity, cell body volume, process length and branching pattern) and tumor volume. RESULTS Clodronation efficacy was high, depending on duration of treatment and length of cultivation. iPSCs and tumor cells integrated into the slice very well. The presence of tumor cells led to an increased sphericity of iPSC-dervied microglia and to an increased cell body volume. Branching pattern and process length did not differ between both conditions. Tumor volume was significantly larger when iPSC-derived microglia were present. This was found in various glioma cells lines and also in patient derived cells. CONCLUSION The newly established humanized ex-vivo brain slice system was shown to be feasible. The method successfully allows to study the interaction between human TAMs and tumor cells. Microglia foster tumor growth not only in murine glioma models, but also in a human paradigm. The humanized ex-vivo brain slice model therefore is the optimal basis to study the role human-specific genes in TAM-glioma interaction.

Mutant IκBα Suppresses Hypoxia-Induced VEGF Expression Through Downregulation of HIF-1α and COX-2 in Human Glioma Cells

2005 ◽  
Vol 15 (3) ◽  
pp. 139-149 ◽  
Author(s):  
Yoshihira Kimba ◽  
Tatsuya Abe ◽  
Jian Liang Wu ◽  
Ryo Inoue ◽  
Minoru Fukiki ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Vegf Expression ◽  
Cox 2

scholarly journals Differential expression and role of p21cip/waf1 and p27kip1 in TNF-α-induced inhibition of proliferation in human glioma cells

2007 ◽  
Vol 6 (1) ◽  
pp. 42 ◽  
Author(s):  
Pabbisetty Kumar ◽  
Anjali Shiras ◽  
Gowry Das ◽  
Jayashree C Jagtap ◽  
Vandna Prasad ◽  
...  
Keyword(s):  
Differential Expression ◽  
Glioma Cells ◽  
Human Glioma ◽  
Human Glioma Cells ◽  
Inhibition Of Proliferation ◽  
Tnf Α
Sign in / Sign up

Export Citation Format

Share Document