Tissue CEA determination and cytophotometric DNA analysis of colorectal mucosa in patients with colorectal cancer

1985 ◽  
Vol 15 (6) ◽  
pp. 449-454 ◽  
Author(s):  
Hiroshi Suzuki ◽  
Eiichi Honda ◽  
Koichi Matsumoto ◽  
Keiji Iriyama
Keyword(s):  
Colorectal Cancer ◽  
Dna Analysis ◽  
Colorectal Mucosa ◽  
Cytophotometric Dna Analysis ◽  
Tissue Cea

scholarly journals High expression of Wnt7a protein predicts a poor survival in patients with colorectal carcinoma

2020 ◽  
Author(s):  
Congcong Li ◽  
Peilin Cui ◽  
Xiaowei Dou ◽  
Hongli Li ◽  
Jiahuan Sun ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Malignant Tumors ◽  
Integration Site ◽  
Normal Mucosa ◽  
Lymph Node Involvement ◽  
Colorectal Adenomas ◽  
Future Research ◽  
Colorectal Mucosa ◽  
Staining Score

Abstract Background: Colorectal cancer is one of the most common malignant tumors in China, and the number of new cases and the number of cases of deaths has increased annually. However, its pathogenesis is still unclear. Wnt7a is a member of the wingless-type MMTV integration site family, and it plays an important role in tumorigenesis and development by controlling cell proliferation and differentiation as a secreted glycoprotein. Whether Wnt7a has the properties of an oncogene or not is an important focus for future research as this target has diverse roles in different tumors.Methods: Wnt7a protein expression in normal colorectal mucosa and colorectal tumors was detected via immunohistochemistry and Western blot analysis. Univariate and multivariate analyses were used to explore the associations between Wnt7a staining score and various clinical parameters.Results: Wnt7a was strongly expressed in colorectal cancer tissues but weakly expressed in adjacent normal mucosa and colorectal adenomas. The level of Wnt7a expression was correlated with lymph node involvement (P < 0.001), Duke stage (P < 0.001), and cell differentiation (P < 0.001). Knockdown of Wnt7a inhibits proliferation of colon cancer cells and inhibits the ability of both colon cancer cell lines to migrate.Conclusions: Collectively, our results present evidence that Wnt7a is associated with an unfavorable prognosis of colorectal cancer.

Minimal residual disease by circulating tumor DNA analysis for colorectal cancer patients receiving radical surgery: An initial report from CIRCULATE-Japan.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. 3608-3608
Author(s):  
Hiroki Yukami ◽  
Yoshiaki Nakamura ◽  
Jun Watanabe ◽  
Masahito Kotaka ◽  
Kentaro Yamazaki ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Analysis ◽  
Residual Disease ◽  
Clinical Stage ◽  
Circulating Tumor Dna ◽  
Patient Specific ◽  
Detection Rates ◽  
Initial Report ◽  
Post Surgery ◽  
Tumor Dna

3608 Background: Circulating tumor DNA (ctDNA) analysis can be used to predict the risk of recurrence by detecting molecular residual disease (MRD) in patients with colorectal cancer (CRC). We are conducting a prospective observational study to monitor MRD status in patients with clinical stage II–IV or relapsed CRC amenable to radical surgical resection (GALAXY study), as part of the CIRCULATE-Japan, a nationwide ctDNA-guided precision adjuvant therapy project. Methods: Analysis of ctDNA is being performed at pre- and post-surgery timepoints and will continue periodically for up to 2 years using Signatera, a personalized, tumor-informed ctDNA assay that is designed to track 16 patient-specific somatic variants based on whole-exome sequencing of tumor tissue. The association of peri-operative ctDNA status with clinicopathological characteristics was investigated. Results: As of January 13, 2021, 941 patients have been enrolled in the GALAXY study, of which 400 patients had their pre-operative ctDNA status evaluated. Of the 400 patients, baseline ctDNA was detected in 92% (367/400) of the patients: consisting of 35 patients with pathological stage (pStage) I, 135 with pStage II, 152 with pStage III, and 78 with pStage IV or relapsed disease (pStage IV/R). Patient-specific Signatera assays targeting 16 variants were designed for 100% of the patients. Out of the 6400 designed variants 99.3% passed quality control in the plasma analysis and produced the final results. Among 4425 genes selected for 400 patients, 3330 genes were selected for only one patient, while TP53 was the most commonly selected in 113 patients (28%). Median ctDNA levels, measured in mean tumor molecules per mL of plasma and ctDNA detection rate, stratified by stage are presented in table. Positive ctDNA status post-surgery was significantly associated with advanced pStage, pT and pN, and lymphovascular invasion. Of the 13 patients with recurrence, 10 were detected with a positive ctDNA at 4-weeks post-surgery, before confirmation of recurrence by the radiological imaging. Conclusions: Preoperative ctDNA detection rates were observed to be in >90% in patients with pStage II–III by personalized ctDNA assay based on unique somatic variants, specific to each patient. ctDNA- based MRD detected post-surgery (4W) was significantly associated with certain known clinicopathological factors for recurrence with ctDNA positivity associated with a very short-term of recurrence. Clinical trial information: 000039205. [Table: see text]

Cytophotometric DNA Analysis of Superficial and Advanced Carcinoma of the Esophagus

1988 ◽  
pp. 31-34 ◽  
Author(s):  
K. Inokuchi ◽  
H. Kuwano ◽  
K. Sugimachi ◽  
Y. Koga ◽  
M. Kitamura ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Carcinoma Of The Esophagus ◽  
Advanced Carcinoma ◽  
Cytophotometric Dna Analysis

scholarly journals Circulating Tumor DNA Analysis in Colorectal Cancer: From Dream to Reality

2019 ◽  
pp. 1-14 ◽  
Author(s):  
Carlotta Antoniotti ◽  
Filippo Pietrantonio ◽  
Salvatore Corallo ◽  
Filippo De Braud ◽  
Alfredo Falcone ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Analysis ◽  
Circulating Tumor Dna ◽  
Tumor Dna

scholarly journals Multiple Signatures of the JC Polyomavirus in Paired Normal and Altered Colorectal Mucosa Indicate a Link with Human Colorectal Cancer, but Not with Cancer Progression

2019 ◽  
Vol 20 (23) ◽  
pp. 5965 ◽  
Author(s):  
Elena Uleri ◽  
Claudia Piu ◽  
Maurizio Caocci ◽  
Gabriele Ibba ◽  
Francesca Sanges ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Progression ◽  
Simultaneous Detection ◽  
Normal Mucosa ◽  
T Antigen ◽  
Jc Polyomavirus ◽  
Paired Samples ◽  
Colorectal Mucosa ◽  
Viral Protein 1 ◽  
Coding Control

The JC polyomavirus (JCV) has been repeatedly but discordantly detected in healthy colonic mucosa, adenomatous polyps, and colorectal cancer (CRC), and proposed to contribute to oncogenesis. The controversies may derive from differences in JCV targets, patient’s cohorts, and methods. Studies of simultaneous detection, quantification, and characterization of JCV presence/expression in paired samples of normal/altered tissues of the same patient are lacking. Therefore, we simultaneously quantified JCV presence (DNA) and expression (mRNA and protein) of T-antigen (T-Ag), Viral Protein 1 (Vp1), and miR-J1-5p in paired normal/altered tissues of CRC or polyps, and from controls. JCV signatures were found in most samples. They increased in patients, but were higher in normal mucosa than in corresponding polyp or CRC lesions. JCV non-coding control region (NCCR) DNA rearrangements increased in CRC patients, also in normal mucosa, thus before the onset of the lesion. A new ∆98bp NCCR DNA rearrangement was detected. T-Ag levels were higher in normal mucosa than in adenoma and adenocarcinoma lesions, but decreased to levels of controls in established CRC lesions. In CRC, miR-J1-5p expression decreased with CRC progression. Vp1 expression was not detected. The data indicate a JCV link with the disease, but possible JCV contributes to oncogenesis should occur at pre-polyp stages.

scholarly journals Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Electronics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1805
Author(s):  
Sónia Carvalho ◽  
Isa Carneiro ◽  
Rui Henrique ◽  
Valery Tuchin ◽  
Luís Oliveira
Keyword(s):  
Colorectal Cancer ◽  
Optical Properties ◽  
Wavelength Dependence ◽  
Biological Tissues ◽  
Diagnostic Methods ◽  
Optical Methods ◽  
Published Data ◽  
Spectral Measurements ◽  
Absorption Bands ◽  
Colorectal Mucosa

The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method, and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

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