Computerized optimization of RP-HPLC separation with nonaqueous or partially aqueous mobile phases

1997 ◽  
Vol 44 (9-10) ◽  
pp. 529-537 ◽  
Author(s):  
S. Heinisch ◽  
E. Lesellier ◽  
C. Podevin ◽  
J. L. Rocca ◽  
A. Tchapla
Keyword(s):  
Hplc Separation ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Aqueous Mobile Phases

Occurrence and behavior of system peaks in RP HPLC with solely aqueous mobile phases

2009 ◽  
Vol 32 (17) ◽  
pp. 2864-2870 ◽  
Author(s):  
Květa Kalíková ◽  
Vlastimil Hruška ◽  
Jana Svobodová ◽  
Richard Chudoba ◽  
Bohuslav Gaš ◽  
...  
Keyword(s):  
Rp Hplc ◽  
System Peaks ◽  
Mobile Phases ◽  
And Behavior ◽  
Aqueous Mobile Phases

Theoretical Interpretation of High-Performance Chromatographic Data for a Heterogeneous Series of Compounds

1992 ◽  
Vol 57 (1) ◽  
pp. 33-45
Author(s):  
Vladimír Jakuš
Keyword(s):  
Experimental Data ◽  
High Performance ◽  
Theoretical Interpretation ◽  
Retention Data ◽  
Theoretical Evaluation ◽  
New Approach ◽  
Free Energy Of Solvation ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Reversed Phases

A new approach to theoretical evaluation of the Gibbs free energy of solvation was applied for estimation of retention data in high-performance liquid chromatography on reversed phases (RP-HPLC). Simple and improved models of stationary and mobile phases in RP-HPLC were employed. Statistically significant correlations between the calculated and experimental data were obtained for a heterogeneous series of twelve compounds.

scholarly journals Development and Validation of Stability-Indicating RP-HPLC Method for the Estimation of Lenalidomide and its Impurities in Oral Solid Dosage Form

2019 ◽  
Vol 35 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Somana Siva Prasad ◽  
G. V. Krishna Mohan ◽  
A. Naga Babu
Keyword(s):  
Limit Of Detection ◽  
Degradation Products ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Stability Robustness ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Limit Of Quantitation ◽  
Successful Separation ◽  
Quality By Design Approach

In this study, a novel, simple and precise RP-HPLC method has been developed for the quantitative analysis of Lenalidomide (LLM) in pharmaceutical formulations using analytical quality by design approach. An X-bridge-C18 column (150 mm × 4.6 mm × 3.5 µ) with mobile phases containing a Potassium dihydrogen orthophosphate anhydrous buffer and methanol in the ratio of (90:10 v/v) and (35:65 v/v) are used for the estimation of LLM and its degradation products. The flow rate of 0.8 mL/min is maintained and all degradation studies are performed at 210 nm using photodiode array (PDA) detector. Method Validation is carried out according to International Council for Harmonisation (ICH) guidelines and the parameters namely; precision, accuracy, specificity, stability, robustness, linearity, limit of quantitation (LOQ) and limit of detection (LOD) are evaluated. The present developed RP-HPLC method shows the purity angle of peaks is less than their threshold angle, signifying that it to be suitable for stability studies. Hence, the developed method can be used for the successful separation of LLM and its impurities in the pharmaceutical dosage formulations.

A TEST OF ROBUSTNESS IN IIR-RP-HPLC SEPARATION OF NINE PRIORITY POLLUTANT PHENOLS

2001 ◽  
Vol 24 (3) ◽  
pp. 341-353 ◽  
Author(s):  
E. Marengo ◽  
M. C. Gennaro ◽  
V. Gianotti ◽  
S. Angelino
Keyword(s):  
Hplc Separation ◽  
Priority Pollutant ◽  
Rp Hplc

scholarly journals Validation of developed method by RP-HPLC for simultaneous estimation of famotidine and ibuprofen in human plasma and studying the stability of the drugs in plasma

2018 ◽  
Vol 12 (1) ◽  
pp. 34-48
Author(s):  
K. S Ashutosh ◽  
D. Manidipa ◽  
R. J. V. L. N. Seshagiri ◽  
S. D. Gowri
Keyword(s):  
Human Plasma ◽  
Mobile Phase ◽  
Simultaneous Estimation ◽  
Hplc Separation ◽  
Reverse Phase ◽  
Rp Hplc ◽  
The Stability ◽  
Sodium Dihydrogen ◽  
Isocratic Mode

The RP-HPLC separation was carried out by reverse phase chromatography on a Symmetry C18 (4.6 x 150 mm, 3.5 μm, make: XTerra) with a mobile phase composed of sodium dihydrogen ortho phosphate [pH 2.5] and acetonitrile in the ratio of 30:70 v/v in an isocratic mode at a flow rate of 1.2 mL/min. The run time was maintained for 8.0 min. The detection was monitored at 236 nm. The accuracy was calculated in human plasma and the % recovery was found 99.80 - 99.85 for famotidine and 99.56 -99.85.5 for ibuprofen and reproducibility was found to be satisfactory. The calibration curve for famotidine in human plasma was linear over 3.32 to 6.65 μg/mL and 100- 200 μg/mL for ibuprofen in human plasma respectively. The inter-day and intra-day precision in human plasma was found within limits. The proposed method has adequate sensitivity, reproducibility, and specificity for the determination of famotidine and ibuprofen in plasma. The LLOQ obtained by the proposed method in human plasma were 1.24 and 5.0 μg/mL for famotidine and ibuprofen respectively. The proposed method is simple, fast, accurate, and precise for the quantification of famotidine and ibuprofen in plasma as per the ICH guidelines.Kathmandu University Journal of Science, Engineering and TechnologyVol. 12, No. I, June, 2016, Page: 34-48

Improved RP-HPLC separation of Hg2+and CH3Hg+using a mixture of thiol-based mobile phase additives

2012 ◽  
Vol 47 (1) ◽  
pp. 149-154 ◽  
Author(s):  
Jeffrey D. Meers ◽  
Elham Zeini Jahromi ◽  
Belinda Heyne ◽  
Jürgen Gailer
Keyword(s):  
Mobile Phase ◽  
Hplc Separation ◽  
Mobile Phase Additives ◽  
Rp Hplc
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