The osmotic permeability of a tubule wall

1988 ◽  
Vol 50 (5) ◽  
pp. 547-558 ◽  
Author(s):  
Geoffrey K. Aldis
Keyword(s):  
Osmotic Permeability ◽  
Tubule Wall

Fluid reabsorption by Necturus proximal tubule perfused with solutions of normal and reduced osmolarity

1982 ◽  
Vol 215 (1201) ◽  
pp. 411-431 ◽  
Keyword(s):  
Proximal Tubule ◽  
Experimental Results ◽  
Fluid Absorption ◽  
Perfusion Fluid ◽  
Fluid Reabsorption ◽  
Osmotic Permeability ◽  
Osmotic Equilibration ◽  
Tubule Wall ◽  
Na Uptake ◽  
Necturus Proximal Tubule

Fluid absorption in Necturus proximal tubule was studied when the kidneys were perfused with solutions of different osmolarities. The rate of fluid absorption was inversely proportional to the perfusion fluid osmolarity, while Na uptake remained constant. No difference was detected between the collected and injected luminal fluid, i. e. reabsorption was isotonic at normal and reduced osmolarities. The transtubular osmotic permeability remained fairly constant under the different per­fusion osmolarities. Using our experimental results to test various models based on osmotic equilibration across the tubule wall we show that none of these provides an adequate mechanism for fluid absorption in this epithelium.

scholarly journals Water and Deuterium Oxide Permeability through Aquaporin 1: MD Predictions and Experimental Verification

2007 ◽  
Vol 130 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Artem B. Mamonov ◽  
Rob D. Coalson ◽  
Mark L. Zeidel ◽  
John C. Mathai
Keyword(s):  
Deuterium Oxide ◽  
Diffusion Constant ◽  
Structural Data ◽  
Md Simulations ◽  
Water Model ◽  
Aquaporin 1 ◽  
Self Diffusion ◽  
Osmotic Permeability ◽  
Protein Channels ◽  
And Diffusion

Determining the mechanisms of flux through protein channels requires a combination of structural data, permeability measurement, and molecular dynamics (MD) simulations. To further clarify the mechanism of flux through aquaporin 1 (AQP1), osmotic pf (cm3/s/pore) and diffusion pd (cm3/s/pore) permeability coefficients per pore of H2O and D2O in AQP1 were calculated using MD simulations. We then compared the simulation results with experimental measurements of the osmotic AQP1 permeabilities of H2O and D2O. In this manner we evaluated the ability of MD simulations to predict actual flux results. For the MD simulations, the force field parameters of the D2O model were reparameterized from the TIP3P water model to reproduce the experimentally observed difference in the bulk self diffusion constants of H2O vs. D2O. Two MD systems (one for each solvent) were constructed, each containing explicit palmitoyl-oleoyl-phosphatidyl-ethanolamine (POPE) phospholipid molecules, solvent, and AQP1. It was found that the calculated value of pf for D2O is ∼15% smaller than for H2O. Bovine AQP1 was reconstituted into palmitoyl-oleoyl-phosphatidylcholine (POPC) liposomes, and it was found that the measured macroscopic osmotic permeability coefficient Pf (cm/s) of D2O is ∼21% lower than for H2O. The combined computational and experimental results suggest that deuterium oxide permeability through AQP1 is similar to that of water. The slightly lower observed osmotic permeability of D2O compared to H2O in AQP1 is most likely due to the lower self diffusion constant of D2O.

A Study of the Malpighian Tubules of the Pill Millipede, Glomeris marginata (Villers)

1974 ◽  
Vol 60 (1) ◽  
pp. 41-51
Author(s):  
PATRICIA ANNE FARQUHARSON
Keyword(s):  
Size Distribution ◽  
Fluid Transport ◽  
Molecular Size ◽  
Malpighian Tubules ◽  
Inverse Relation ◽  
Fluid Production ◽  
Molecular Size Distribution ◽  
Molecular Sieving ◽  
Tubule Fluid ◽  
Tubule Wall

1. Tubule fluid:medium ratios (TF/M) have been measured for inulin, glucose, LMWD and HMWD. These TF/M ratios were surprisingly high. 2. The tubule appears to act as a molecular filter; that is to say, molecules move through the tubule wall in inverse relation to their size. This is best illustrated using polyvinyl pyrrolidone as a tracer. The molecular size distribution of PVP fractions present in tubule fluid differs markedly from the molecular size distribution of PVP in the bathing Ringer. 3. No correlation can be made between the inulin and glucose TF/M and the rate of fluid production. However, the inverse relationship between TF/M and rate of fluid production for dextrans indicates a molecular sieving effect. 4. The significance of these results is discussed with reference to models of fluid transport.

Net Fluxes of Water in the Isolated Gills of Anguilla Dieffenbachii

1974 ◽  
Vol 60 (3) ◽  
pp. 769-781
Author(s):  
T. J. SHUTTLEWORTH ◽  
R. F. H. FREEMAN
Keyword(s):  
Sea Water ◽  
Water Flux ◽  
Freshwater Teleost ◽  
Osmotic Permeability ◽  
Direct Measurements ◽  
Net Flux ◽  
Anguilla Dieffenbachii ◽  
Net Fluxes ◽  
Freshwater Eels ◽  
Net Water Flux

1. Measurements of net flux of water have been made on isolated gills removed from freshwater-adapted and seawater-adapted eels and incubated in various media of differing osmotic pressure. 2. From these measurements it has been possible to determine the osmotic permeability coefficient of the gill directly from the net water flux. The values obtained (0.50±0.14x10-5 cm.sec-1 for freshwater eels and 0.43±0.07x10-5 cm.sec-1 for seawater-adapted eels) indicate that there was no significant change in this parameter on adaptation of the eels to sea water. 3. The direct measurements made of the net water flux across the isolated gills appear to be compatible with the osmoregulatory pattern of eels as deduced by other workers using different techniques. In particular they illustrate and further emphasize the significance of drinking in the freshwater fish. 4. Calculations indicate that, for a freshwater teleost, the osmotic and ionic problems caused by drinking in fresh water have an insignificant energetic effect and hence, energetically, it matters little to the fish whether it drinks or not.

Progesterone inhibition of water permeability in Bufo arenarum oocytes and urinary bladder

1996 ◽  
Vol 270 (5) ◽  
pp. F880-F885 ◽  
Author(s):  
P. Ford ◽  
G. Amodeo ◽  
C. Capurro ◽  
C. Ibarra ◽  
R. Dorr ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Xenopus Laevis ◽  
Water Permeability ◽  
Water Channel ◽  
Water Channels ◽  
Toad Urinary Bladder ◽  
Osmotic Permeability ◽  
Bufo Arenarum ◽  
Mrna Extraction

The ovarian oocytes from Bufo arenarum (BAO) but not those from Xenopus laevis (XLO) would have water channels (WC). We now report that the injection of the mRNA from BAO into the oocytes from XLO increased their water osmotic permeability (Pi) (reduced by 0.3 mM HgCl2 and reversed by 5 mM beta-mercaptoethanol). A 30-min challenge with progesterone induced, 18 h later, a reduction of the mercury-sensitive fraction of Pf in the BAO (but not in XLO). The mRNA from BAO pretreated with progesterone lost its capacity to induce WC in the XLO, but the hormone did not affect the expression of the WC in XLO previously injected with the mRNA from BAO. Pf was also measured in urinary bladders of BAO. Eighteen hours after a challenge with progesterone, a reduction in the hydrosmotic response to oxytocin was observed. Finally, the mRNA from the urinary bladder of BAO was injected into XLO. An increase in Pf was observed. This was not the case if, before the mRNA extraction, the bladders were treated with progesterone. We conclude that the BAO WC share progesterone sensitivity with the oxytocin-regulated water channel present in the toad urinary bladder.

Flow along the Gut and Intestinal Absorption of Salt and Water in Euryhaline Teleosts: A Theoretical Analysis

1974 ◽  
Vol 60 (2) ◽  
pp. 557-566
Author(s):  
K. KRISTENSEN ◽  
E. SKADHAUGE
Keyword(s):  
Water Absorption ◽  
Water Flow ◽  
Permeability Coefficient ◽  
Absorption Rate ◽  
Plasma Osmolality ◽  
European Eel ◽  
Drinking Rate ◽  
Osmotic Permeability ◽  
Nacl Absorption ◽  
Concentration Changes

1. In euryhaline teleosts the transmural salt and water flow and the flow and concentration changes along the gut were simulated by analogue computation. The purpose was to elucidate the interaction of and sensitivity to the parameters of the system particularly with respect to intestinal water absorption. The simulations were based on data obtained from the yellow European eel, the rainbow trout and the cyprinodont Aphanius dispar. 2. When the experimental values for drinking rate, maximal NaCl absorption rate and concentration at half-maximal absorption rate, osmotic permeability coefficient, solute-linked water flow, and concentrations in the gut were used in the model, good consistency was achieved, and predictions could be made. 3. The simulations demonstrated a close linkage between drinking rate and maximal NaCl absorption rate. A large water absorption was only possible close to an optimal drinking rate for each value of maximal NaCl transport rate. The water absorption was little sensitive to the osmotic permeability coefficient of the intestinal wall. 4. As a means of adaptation to waters of high salinity an increase in maximal NaCl absorption rate was shown to be very costly for energetic reasons. This supports indirectly the concept that the osmotic permeability of the gills must go down. The increase in plasma osmolality was a useful part of the adaptation.

The Reduced Glucose Permeability of the Isolated Malpighian Tubules of the Blowfly Calliphora Vomitoria

1975 ◽  
Vol 62 (2) ◽  
pp. 327-340
Author(s):  
G. KNOWLES
Keyword(s):  
Molecular Weight ◽  
Malpighian Tubule ◽  
Malpighian Tubules ◽  
Glucose Reabsorption ◽  
Glucose Excretion ◽  
Tubule Wall

The excretion of several sugars by the isolated Malpighian tubules of Calliphora vomitoria has been investigated. The isolated tubules do not excrete glucose or trehalose at rates similar to those of sugars of the same molecular weight. Trehalose can be hydrolysed to glucose as it traverses the tubule wall. It is proposed that glucose can be reabsorbed by the tubule. Evidence is presented to suggest that glucose reabsorption can be saturated. Phloridzin was found to increase the rate of glucose excretion by the isolated Malpighian tubule.

Temperature-dependent osmotic permeability in glycoproteion containing liposomes

1988 ◽  
Vol 13 (2) ◽  
pp. 85-89 ◽  
Author(s):  
Vassil Z. Neitchev ◽  
Fran�ois A. Bideaud
Keyword(s):  
Temperature Dependent ◽  
Osmotic Permeability
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